The 24-Hour Posttransfusion Survival and Lifespan of Autologous Baboon Red Cells Treated with Inositol Hexaphosphate-Polyethylene Glycol or Inositol Hexaphosphate-Adenosine Triphosphate-Polyethylene Glycol to Decrease Oxygen Affinity

Abstract

Baboon red cells were treated to reduce oxygen affinity by an osmotic-pulse procedure using dimethylsulfoxide. Inositol hexaphosphate (IHP) alone or IHP and adenosine triphosphate (ATP) were incorporated into the red cells in the presence of polyethylene glycol (PEG). The procedure produced variable increases in the red cell P(50) value, i.e., the partial pressure of oxygen at which 50% of the hemoglobin was saturated. The effect of treatment of autologous baboon red cells on the 24-hour posttransfusion survival value and lifespan T(50) value was measured using a double-label procedure. The data demonstrate that the increase in the P(50) value of treated red cells was negativelycorrelated with the 24-hour posttransfusion survival value; the higher the P(50) value, the poorer the 24-hour posttransfusion survival value. The 24-hour posttransfusion survival value for nontreated baboon red cells was 90% and the T(50) value was 14 days. The IHP-ATP-PEG-treated red cells had significantly higher red cell ATP levels than did IHP-PEG-treated red cells. The 24-hour posttransfusion survival value was 68% for the IHP-ATP-PEG treated red cells and 52% for the IHP-PEG-treated red cells when the increase in P(50) ranged from 10 to 20 mm Hg; the lifespan T(50) value for both the IHP-ATP-PEG-treated red cells and the IHP-PEG-treated red cells was 15 days. Osmotic pulse treatment produced significant red cell injury manifested by the 24-hour posttransfusion survival value. However, modification of the RBC with IHP-PEG or IHP-ATP-PEG to decrease hemoglobin affinity for oxygen did not affect their lifespan.

Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets

“…For the past 30 years our laboratory has been using the baboon in studies for assessing methods to disinfect, modify and preserve autologous red blood cells and platelets [1–10]. The study reported here was carried out to compare the in vivo survival of fresh, cryopreserved and liquid‐preserved platelets, and to evaluate the distribution of radioactivity over the spleen and liver to determine the site of removal of the non‐viable platelets.…”

The survival and function of baboon red blood cells, platelets, and plasma proteins: a review of the experience from 1972 to 2002 at the Naval Blood Research Laboratory, Boston, Massachusetts

“…Osmotic pulse treatment produced significant RBC injury manifested by the reduced 24‐hour posttransfusion survival value. Modification of the RBCs with IHP‐PEG or IHP‐ATP‐PEG to decrease Hb affinity for oxygen, however, did not affect their life span 32 …”

“…Baboon RBCs were treated to reduce oxygen affinity by an osmotic‐pulse procedure with DMSO 32 . Inositol hexaphosphate (IHP) alone or IHP and ATP were incorporated into the RBCs in the presence of polyethylene glycol (PEG).…”

Survival and function of baboon RBCs released from clotted blood and washed before autologous transfusion

“…In vitro recovery of the RBCs was assessed by comparing the volume of RBCs in the collected blood with that recovered after washing 9 . Total Hb concentration, supernatant Hb concentration, and microhematocrit were measured, together with volumes of blood, to determine the in vitro recovery values 9,11,13 . Heparinized blood samples were collected at the time of blood collection and after washing of the released RBCs to assay for RBC 2,3 DPG, ATP, and p50 14,15 …”

“…All units were washed before autologous transfusion. Baboon blood was used because of the similarity between baboon RBCs and human RBCs in their response to cellular injury 9–11 …”