Survival and function of baboon RBCs released from clotted blood and washed before autologous transfusion

Valeri, C. R.; Ragno, Gina; Pivacek, Linda E.; Dennis, Richard C.; Hechtman, Herbert B.; Khuri, Shukri F.

doi:10.1046/j.1537-2995.2001.41111384.x

Cited by 4 publications

(10 citation statements)

References 21 publications

(20 reference statements)

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“…For the past 30 years our laboratory has been using the baboon in studies for assessing methods to disinfect, modify and preserve autologous red blood cells and platelets [1–10]. The study reported here was carried out to compare the in vivo survival of fresh, cryopreserved and liquid‐preserved platelets, and to evaluate the distribution of radioactivity over the spleen and liver to determine the site of removal of the non‐viable platelets.…”

Section: Introductionmentioning

confidence: 99%

Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets

et al. 2002

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Cryopreserved platelets and liquid-preserved platelets stored at 22 degrees C for 5 days had reduced survival 2 h post-transfusion and reduced life span values compared to fresh platelets. In addition, the finding of increased radioactivity over the liver in the baboons that received cryopreserved and liquid-preserved platelets suggested that the liver was the site for removal of the non-viable platelets.

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Section: Introductionmentioning

confidence: 99%

Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets

et al. 2002

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“…The autotransfusion of a volume of plasma treated with urokinase or with urokinase and aprotonin equivalent to 15% of the blood volume did not produce a bleeding diathesis in these healthy baboons. The survival of the autologous baboon RBC in clotted blood treated with 10,000 units of urokinase has been shown to be excellent with normal or slightly reduced oxygen transport function 45 . The safety and therapeutic effectiveness of the nonwashed shed blood is dependent upon the quality and quantity of RBC and plasma that are reinfused.…”

Section: Discussionmentioning

confidence: 99%

Hemostatic Defect in Baboons Autotransfused Treated Plasma to Simulate Shed Blood

et al. 2006

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“…We simulated the effects of clotting and endogenous lysis such as may occur during the collection of shed blood. Urokinase was used for the sole purpose of lysing the clotted baboon blood to release the RBCs for viability and function measurements 34 …”

Section: Studies Performed To Assess the Survival And Function Of Babmentioning

confidence: 99%

“…The 51 Cr t 1/2 value was normal at 14 days, and the RBC oxygen transport function was only slightly reduced. Autologous baboon RBCs isolated from clotted blood treated or not treated with urokinase and washed before transfusion have excellent survival and normal or only slightly reduce oxygen‐transport function 34 …”

Section: Studies Performed To Assess the Survival And Function Of Babmentioning

confidence: 99%

“…The autotransfusion of a volume of plasma treated with urokinase or with urokinase and aprotonin equivalent to 15 percent of the blood volume did not produce a bleeding diathesis in these healthy baboons. The survival of the autologous baboon RBC in clotted blood treated with 10,000 units of urokinase has been shown to be excellent with normal or slightly reduced oxygen transport function 34 . The safety and therapeutic effectiveness of the nonwashed shed blood is dependent on the quality and quantity of RBCs and plasma that are retransfused.…”

Section: Disinfection Of Autologous Baboon Rbcsmentioning

confidence: 99%

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The survival and function of baboon red blood cells, platelets, and plasma proteins: a review of the experience from 1972 to 2002 at the Naval Blood Research Laboratory, Boston, Massachusetts

Valeri¹,

Ragno²

2006

Transfusion

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The studies reported in this monograph were performed between 1972 and 2002 when it was possible to study healthy male and female baboons. A colony of baboons was maintained for 30 years without any adverse events observed in these baboons in the numerous studies that were performed. These protocols were reviewed and approved by the institutional animal care and use committees (IACUC) at the sites where the studies were performed and by the veterinarian services of the U.S. Navy's Bureau of Medicine and Surgery, the Office of Naval Research, and the Department of Defense. The physiology of red blood cells (RBCs), platelets (PLTs), and plasma proteins in the baboon was investigated together with the viability and function of preserved RBCs, PLTs, and plasma proteins. These studies in the baboon could not have been performed in normal volunteers and patients. The data obtained have provided critical information to explain the clinical observations reported in normal volunteers and patients after transfusion of fresh and preserved blood products. These studies were supported by the U.S. Navy's Bureau of Medicine and Surgery and the Office of Naval Research. In addition, the support of the late Congressman J. Joseph Moakley from Massachusetts is acknowledged because without his support many of these studies could not have been performed. The authors acknowledge the contributions of the numerous research collaborators identified in the 52 peer-reviewed publications that cite other funding agencies that supported the research that is reported, the editorial assistance of Ms Cynthia Ann Valeri, and the assistance of Ms Deborah Tattersall who prepared the figures and tables reported in this publication.

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Survival and function of baboon RBCs released from clotted blood and washed before autologous transfusion

Abstract: Autologous baboon RBCs isolated from clotted blood treated or not treated with urokinase and washed before transfusion have excellent survival and normal or only slightly reduced oxygen-transport function.

Cited by 4 publications

References 21 publications

Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets

Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets

Hemostatic Defect in Baboons Autotransfused Treated Plasma to Simulate Shed Blood

The survival and function of baboon red blood cells, platelets, and plasma proteins: a review of the experience from 1972 to 2002 at the Naval Blood Research Laboratory, Boston, Massachusetts

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