The 2021 yearbook of Neurorestoratology

Sharma, Hari Shanker; Chopp, Michael; Chen, Lin; Sarnowska, Anna; Xue, Mengzhou; Ao, Qiang; Siniscalco, Dario; Chen, Lukui; Hawamdeh, Ziad M.; Huang, Hongyun

doi:10.1016/j.jnrt.2022.100008

Cited by 23 publications

(5 citation statements)

References 100 publications

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“…Considering the hyperproliferative property of Mysm1 deficient NSCs, knockdown Mysm1 in NSCs from aged brains may be an ideal strategy for inducing NSC proliferation, and thereby promoting neurogenesis. Neurogenesis is believed to be an important neurorestorative mechanism [ 53 ], the present study revealed a new factor may be a valuable target for brain disorder therapy.…”

Section: Discussionmentioning

confidence: 64%

Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression

Xu,

Qin,

Wang

et al. 2024

Cell Death Dis

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Neural stem cells (NSCs) are critical for brain development and maintenance of neurogenesis. However, the molecular mechanisms that regulate NSC proliferation and differentiation remain unclear. Mysm1 is a deubiquitinase and is essential for the self-renewal and differentiation of several stem cells. It is unknown whether Mysm1 plays an important role in NSCs. Here, we found that Mysm1 was expressed in NSCs and its expression was increased with age in mice. Mice with Mysm1 knockdown by crossing Mysm1 floxed mice with Nestin-Cre mice exhibited abnormal brain development with microcephaly. Mysm1 deletion promoted NSC proliferation and apoptosis, resulting in depletion of the stem cell pool. In addition, Mysm1-deficient NSCs skewed toward neurogenesis instead of astrogliogenesis. Mechanistic investigations with RNA sequencing and genome-wide CUT&Tag analysis revealed that Mysm1 epigenetically regulated Id4 transcription by regulating histone modification at the promoter region. After rescuing the expression of Id4, the hyperproliferation and imbalance differentiation of Mysm1-deficient NSCs was reversed. Additionally, knockdown Mysm1 in aged mice could promote NSC proliferation. Collectively, the present study identified a new factor Mysm1 which is essential for NSC homeostasis and Mysm1-Id4 axis may be an ideal target for proper NSC proliferation and differentiation.

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Section: Discussionmentioning

confidence: 64%

Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression

Xu,

Qin,

Wang

et al. 2024

Cell Death Dis

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“…SCI causes considerable physical and psychological harm to patients and imposes financial burdens on families and society [ 31 ]. Recently, many neurorestorative strategies have been tested to repair those damaged functions and/or structures [ 32 – 35 ], especially virtual reality technology has been used to assist the study of the neural mechanisms that underlie behaviors modulated by the environmental context [ 36 ]. After SCI, an abnormal and continuously active inflammatory response causes substantial neuronal death [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca2+/CaMK2b Pathway

Xia,

Ding,

Zhang

et al. 2023

Neurosci. Bull.

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Spinal cord injury (SCI) is one of the most devastating traumas, and the aberrant proliferation of astrocytes usually causes neurological deficits. However, the mechanism underlying astrocyte over-proliferation after SCI is unclear. Grin2c (glutamate ionotropic receptor type 2c) plays an essential role in cell proliferation. Our bioinformatic analysis indicated that Grin2c and Ca2+ transport functions were inhibited in astrocytes after SCI. Suppression of Grin2c stimulated astrocyte proliferation by inhibiting the Ca2+/calmodulin-dependent protein kinase 2b (CaMK2b) pathway in vitro. By screening different inflammatory factors, interleukin 1α (IL1α) was further found to inhibit Grin2c/Ca2+/CaMK2b and enhance astrocyte proliferation in an oxidative damage model. Blockade of IL1α using neutralizing antibody resulted in increased Grin2c expression and the inhibition of astrocyte proliferation post-SCI. Overall, this study suggests that IL1α promotes astrocyte proliferation by suppressing the Grin2c/Ca2+/CaMK2b pathway after SCI, revealing a novel pathological mechanism of astrocyte proliferation, and may provide potential targets for SCI repair.

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“…When effectively optimized and implemented, this combination of targeted labeling and Raman analysis can specifically focus on organelles of interest, enhancing the obtained Raman spectrum to send signals or produce strong peak bands (1800-2,800 cm −1 ) within the biologically Raman silent spectral region (Adamczyk et al, 2021). Raman cell imaging can offer insight into the chemical composition of biological samples, providing new opportunities for the detection and diagnosis of neurodegenerative diseases (Huang et al, 2021(Huang et al, , 2023Sharma et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Applications of Raman spectroscopy in the diagnosis and monitoring of neurodegenerative diseases

Chen,

Qi,

et al. 2024

Front. Neurosci.

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Raman scattering is an inelastic light scattering that occurs in a manner reflective of the molecular vibrations of molecular structures and chemical conditions in a given sample of interest. Energy changes in the scattered light can be assessed to determine the vibration mode and associated molecular and chemical conditions within the sample, providing a molecular fingerprint suitable for sample identification and characterization. Raman spectroscopy represents a particularly promising approach to the molecular analysis of many diseases owing to clinical advantages including its instantaneous nature and associated high degree of stability, as well as its ability to yield signal outputs corresponding to a single molecule type without any interference from other molecules as a result of its narrow peak width. This technology is thus ideally suited to the simultaneous assessment of multiple analytes. Neurodegenerative diseases represent an increasingly significant threat to global public health owing to progressive population aging, imposing a severe physical and social burden on affected patients who tend to develop cognitive and/or motor deficits beginning between the ages of 50 and 70. Owing to a relatively limited understanding of the etiological basis for these diseases, treatments are lacking for the most common neurodegenerative diseases, which include Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis. The present review was formulated with the goal of briefly explaining the principle of Raman spectroscopy and discussing its potential applications in the diagnosis and evaluation of neurodegenerative diseases, with a particular emphasis on the research prospects of this novel technological platform.

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The 2021 yearbook of Neurorestoratology

Cited by 23 publications

References 100 publications

Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression

Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression

Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca2+/CaMK2b Pathway

Applications of Raman spectroscopy in the diagnosis and monitoring of neurodegenerative diseases

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