Thai herbal formulation ‘Ya-Pit-Samut-Noi’: Its antibacterial activities, effects on bacterial virulence factors and in vivo acute toxicity

Limsuwan, Surasak; Jarukitsakul, Siriporn; Issuriya, Acharaporn; Chusri, Sasitorn; Joycharat, Nantiya; Jaisamut, Patcharawalai; Saising, Jongkon; Jetwanna, Korakot Wichitsa-nguan; Voravuthikunchai, Supayang Piyawan

doi:10.1016/j.jep.2020.112975

Cited by 8 publications

(6 citation statements)

References 68 publications

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“…The importance of controlling bacterial infections cannot be over-emphasized due to their prevailing negative effects in primary health care as well as the complications that arise from bacterial infections related to other diseases. An increasing number of reports illustrate the importance of medicinal plants in the treatment of bacterial infections [ 18 ]. The results indicated that A. ebracteatus extract showed significant antibacterial properties.…”

Section: Discussionmentioning

confidence: 99%

New Insights on Acanthus ebracteatus Vahl: UPLC-ESI-QTOF-MS Profile, Antioxidant, Antimicrobial and Anticancer Activities

et al. 2022

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This study investigated the antioxidant, antimicrobial, anticancer, and phytochemical profiling of extracts from the leaves and stem/root of Acanthus ebracteatus (AE). The total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical-scavenging activity, 2, 2′-azino-Bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activity, metal chelating activities (MCA), ferric reducing antioxidant power (FRAP) and oxygen radical antioxidant capacity (ORAC) were used for antioxidant assessment. The ethanolic extracts of the leaves (AEL-nor) and stem/root (AEWP-nor) without chlorophyll removal and those with chlorophyll removal, using sedimentation process (AEL-sed and AEWP-sed), were prepared. Generally, AEL-sed showed the highest antioxidant activity (FRAP: 1113.2 µmol TE/g; ORAC: 11.52 µmol TE/g; MCA: 47.83 µmol EDTA/g; ABTS 67.73 µmol TE/g; DPPH 498.8 µmol TE/g; TPC: 140.50 mg/GAE g and TFC: 110.40 mg/CE g) compared with other extracts. Likewise, AEL-sed also showed the highest bacteriostatic (MIC) and bactericidal (MBC) effects, as well as the highest anticancer and antiproliferative activity against oral squamous carcinoma (CLS-354/WT) cells. UPLC-ESI-QTOF/MS analysis of AEL-sed and AEWP-sed tentatively identified several bioactive compounds in the extracts, including flavonoids, phenols, iridoids, and nucleosides. Our results provide a potentially valuable application for A. ebracteatus, especially in further exploration of the plant in oxidative stress-related disorders, as well as the application of the plant as potential nutraceuticals and cosmeceuticals.

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Section: Discussionmentioning

confidence: 99%

New Insights on Acanthus ebracteatus Vahl: UPLC-ESI-QTOF-MS Profile, Antioxidant, Antimicrobial and Anticancer Activities

et al. 2022

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“…(with Cola anomala ), and by Limsuwan et al. (with Thai herbal formulation) [ [21] , [22] , [23] ]. The cytotoxicity analysis of the compounds by using a cell line is a widely accepted method.…”

Section: Discussionmentioning

confidence: 99%

Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis

Singh

Sharma

Agarwal

2021

Journal of Ayurveda and Integrative Medicine

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Background Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective The study investigates the anti-biofilm, anti-microbial, and anti-shigellosis activity of a designed formulation. Materials and methods The potential of the formulation against S. flexneri (MTCC 1457) was investigated using a well-diffusion assay. Further, the effect of the designed formulation on bacterial growth and biofilm formation was analyzed by the spectrophotometry method. Anti-quorum sensing activity was analyzed using Chromobacterium violaceum CV026, a bacterial strain. Finally, the cytotoxicity of the formulation was examined by using cell line and brine shrimp lethality assay. Results The MIC value of the aqueous extract of the formulation was 2.4 mg ml −1 and an inhibitory zone of 23 mm was observed. On the other side, the formulation significantly inhibited the bacterial growth, biofilm formation (23.78%), violacein inhibition (27.68%) at 0.6 mg ml −1 concentration (did not significantly affect the growth curves) and was found non-toxic in cell assay and brine shrimp lethality assay. Conclusion According to the result obtained, the designed formulation was found effective and non-toxic, so it can be used to treat shigellosis and Shigella -related infections.

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“…The results showed that all the five extracts are found non-toxic when given up to the dose of 2000 mg/kg. During 14 days of acute oral toxicity study, no mortality, no signs of toxicity, and no behavioral changes were observed (Lipnick et al 1995;Limsuwan et al 2020).…”

Section: Discussionmentioning

confidence: 96%

“…Respiratory tract infection was selected in this study because it is a common disease, especially among children, and is directly correlated with air pollution and industrialization. S. aureus causes severe bacterial pneumonia, secondary disease, to viral infections, which causes widespread mortality and morbidity (Kumar et al 2009). H influenza infection causes swelling of the epiglottis to ensue and progressive erythema.…”

Section: Discussionmentioning

confidence: 99%

Herbal antibacterial remedy against upper respiratory infection causing bacteria and in vivo safety analysis

Singh

Agarwal

2021

Vegetos

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In the present study, the antibacterial activity and in vivo acute toxicity of five different extracts was analyzed. The Minimum Inhibitory Concentration and antibacterial activity of five different extracts, including Terminalia chebula, Camellia sinensis, Glycyrrhiza glabra, Vitis vinifera, and Terminalia arjuna, against three different bacterial species, namely, Streptococcus pyogenes, Haemophilus influenza, and Staphylococcus aureus was analyzed by broth dilution method and agar well diffusion method, respectively. The in vivo acute oral toxicity was analyzed by using Wistar rats. The antibacterial activity of different extracts was calculated by analyzing the zone of inhibition, and the maximum zone of 22 mm and minimum zone of 10 mm were observed against the different upper respiratory infections causing bacteria. The lowest MIC (0.8 mg/ml) found was of Camellia sinensis against the Streptococcus pyogenes and Staphylococcus aureus. No sign of toxicity and mortality was observed during the 14 days of study. The in vitro results of the tested extracts indicate that they possessed antibacterial activity and were found non-toxic. So, it can be used as an alternative for the treatment of upper respiratory infection-causing bacteria.

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Thai herbal formulation ‘Ya-Pit-Samut-Noi’: Its antibacterial activities, effects on bacterial virulence factors and in vivo acute toxicity

Cited by 8 publications

References 68 publications

New Insights on Acanthus ebracteatus Vahl: UPLC-ESI-QTOF-MS Profile, Antioxidant, Antimicrobial and Anticancer Activities

New Insights on Acanthus ebracteatus Vahl: UPLC-ESI-QTOF-MS Profile, Antioxidant, Antimicrobial and Anticancer Activities

Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis

Herbal antibacterial remedy against upper respiratory infection causing bacteria and in vivo safety analysis

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