Tetraethyl Orthosilicate and Acrylic Acid Forming Robust Carboxylic Functionalities on Plastic Surfaces for Biodiagnostics

Abstract

properties of film that is formed as a result of sequential, plasma assisted fragmentation and deposition of vapours of acrylic acid and tetraethyl ortho silicate (TEOS). In this context, TEOS serves as an adhesion layer to the plastic substrate and also as a network-building layer for further cross-linking with acrylic acid. The presence of silanols (Si-OH) can facilitate large uptake of water molecules and cause significant hydration of the layer, which in combination with high total negative charge lowers the non specific binding of biomolecules..-2 -The reactivity of the carboxyl functionalities was confirmed by total internal reflection ellipsometry technique in a reaction with amino terminated ssDNA. Ageing studies of the film showed long term stability over 50 days. Overall, the specific combination of TEOS and acrylic acid deposited by plasma assisted vapour method results in film with an increased percentage of carboxyl groups to 16.4% as compared to that of 6.5% in acrylic acid as determined in the core level C 1s XPS spectrum and a significant increase in signal to noise ratio.

Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

“…The polymerization process is initialized and assisted by plasma and results into a fabrication of relatively homogeneous layer with high density of carboxylic acids. The very low water contact angle of the film is also suggesting that TEOS might be inserted into the sensing -COOH layer, cross linking the polymerized AA molecules and forming abundance of silanols and silyl-ethers [26].…”

Scalable Low-Cost Fabrication of Disposable Paper Sensors for DNA Detection

“… 12 The role of siloxane in anchoring to the plastic and metal surfaces for further plasma functionalization and cross-linking has been established previously. 25 , 26 We carried out the amine functionalization using APTES as the precursor material, since the adhesion of the functionalized coating to the cellulose fibers is critical for repeatable and reproducible bioassay performance. The schematic for binding of APTES to cellulose is shown in Figure 1 c. The deposition time was fixed at 20 s, and no systematic variation was performed to study the effect of deposition time on surface functionalization and DNA hybridization.…”

“…Surface amination of polymer substrates by plasma-enhanced chemical vapor deposition (PECVD) is routinely carried out using allylamine, ammonia, and nitrogen/hydrogen gases. , In low-pressure PECVD, APTES has an advantage over alkyl amine in determining the extent of amination and adhesion strength to surfaces upon rigorous washing . The role of siloxane in anchoring to the plastic and metal surfaces for further plasma functionalization and cross-linking has been established previously. , We carried out the amine functionalization using APTES as the precursor material, since the adhesion of the functionalized coating to the cellulose fibers is critical for repeatable and reproducible bioassay performance. The schematic for binding of APTES to cellulose is shown in Figure c.…”

Preparation of poly(acrylamide‐co‐acrylic acid)/silica nanocomposite microspheres and their performance as a plugging material for deep profile control

“…Moreover, some studies have reported that the CC bonds of acrylic acid (AA) are easily attached to silanol groups at the surface of nanosized SiO 2 which would promote the coating process . The encapsulation of acrylamide (AM) on SiO 2 particles can also be driven by the hydrogen‐bonding interaction between the hydroxyl group on the surface of SiO 2 core and the amide unit of AM during the polymerization .…”