Tetrabromobisphenol A activates inflammatory pathways in human first trimester extravillous trophoblasts in vitro

Abstract

Tetrabromobisphenol A (TBBPA) is a widely used flame retardant. Despite the presence of TBBPA in gestational tissues and the importance of proper regulation of inflammatory networks for successful pregnancy, there is no prior study on the effects of TBBPA on inflammatory responses in gestational tissues. The present study aimed to investigate TBBPA activation of inflammatory pathways, specifically cytokine and prostaglandin production, in the human first trimester placental cell line HTR-8/SVneo. TBBPA enhanced release of interleukin (IL)-6, IL-8, and prostaglandin E2 (PGE2), and suppressed TGF-β release in HTR-8/SVneo cells. The lowest effective concentration was 10 μM TBBPA. A commercial immune response PCR array revealed increased expression of genes involved in inflammatory pathways stimulated by TBBPA in HTR-8/SVneo cells. Because proper regulation of inflammatory mediators in the gestational compartment is necessary for normal placental development and successful pregnancy, further investigation on the impact of TBBPA-stimulated responses on trophoblast function is warranted.

Protective effect of (±)α-tocopherol on brominated diphenyl ether-47-stimulated prostaglandin pathways in human extravillous trophoblasts in vitro

“…This work was performed with the HTR-8/SVneo cell line (Graham et al, 1993). The HTR-8/SVneo cell line was derived from first trimester placentae and has provided a useful cell culture model for studies of EVT cellular responses (Liu et al , 2012; Wang et al , 2012; Weber et al , 2013) and initial investigations of toxicant actions on EVTs (Park et al , 2014a; Tetz et al , 2013a). …”

Analysis of the effects of polyunsaturated fatty acids on transporter expressions using a PCR array: Induction of xCT/SLC7A11 in human placental BeWo cells

“…Berveiller et al revealed the changes in some drug transporters during differentiation of cytotrophoblast to syncytiotrophoblast [27]. Park et al demonstrated that tetrabromobisphenol A increased some genes involved in inflammatory in first trimester extravillous trophoblast HTR-8/SVneo cells using a PCR array kit focused on an immune response pathway [28]. Using the drug transporter PCR array kit, we revealed that SLC7A11 was upregulated by treatment with AA and EPA.…”

“…In the present study, a PCR array kit was applied to thoroughly assess the profiles of transporter expression. There are many reports that investigate gene expression in a specific pathway by PCR array in various type of cells [25][26][27][28]. Berveiller et al revealed the changes in some drug transporters during differentiation of cytotrophoblast to syncytiotrophoblast [27].…”

Tetrabromobisphenol A – Toxicity, environmental and occupational exposures

“…Wykazano również zwiększoną ekspresję genów zaangażowanych w szlaki zapalne. Najniższe stęże-nie TBBPA, powodujące zmiany w aktywacji czynników prozapalnych, to 10 μM [55].…”