Tests for Kinetin‐Like Growth Promoting Activities of Triacanthine and Its Isomer, 6‐(γ,γ‐Dimethylallylamino)‐Purine

Rogozińska, Janina; Helgeson, John P.; Skoog, Folke

doi:10.1111/j.1399-3054.1964.tb09029.x

Cited by 59 publications

(12 citation statements)

References 8 publications

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“…single poinlt bioassavs based oni yields of tissues after a constant, relatively long, growth period (10,13,15,16,21 of the bioassay may be related to the fact that only a small increase in logarithmic growth rate occurs with each increment of additional cytokinin above about 10-2 FM. This will result in final weight yields that differ very little with change in cvtokinin con-centration above 10-2 -UM even though all tissues are weighed while in log phase.…”

Section: Discussionmentioning

confidence: 99%

Control of Logarithmic Growth Rates of Tobacco Callus Tissue by Cytokinins

1969

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A bstract. The growth rates of tobacco callus tissues on media containing 10-to 10 juwr 6-(y,y-dimethylallvlamino)purine (2iP) were measured. At concentrations of 10-4 AM and above growth rates were exponential and dependent on cytokinin concentration. At 2iP concentrations of 10-4 to 0.33 AM, the exponential rate was maintained for 4 to 5 doublings of fresh and dry weight. After this period a linear phase, resulting in approximately 1 doubling of weight, occurred. The growth of tissues on media containing higher than 0.33 uNI 2iP was exponential for only about 15 days. At the end of this time, and well before they achieved half their final weight, they exhibited growth which was less rapid than logarithmic but more rapid than linear. Comparisons with zeatin, 6-benzylaminopurine and kinetin indicated that, although the maximum growth rates obtained with relatively high concentrations (0.1-1 jIM) were similar, the naturally occurring cytokinins, 2iP and zeatin, promoted faster rates at lower concentrations (10--10-2 ;LNI) than did 6-benzylaminopurine and kinetin. (6,13,16,20

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Section: Discussionmentioning

confidence: 99%

Control of Logarithmic Growth Rates of Tobacco Callus Tissue by Cytokinins

1969

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“…Furthermore the effectiveness at very low concentratiolns of the kinini, N.6-(,y,y-dimethylallyl amino)-purine (21) and the activity of low levels of kinetin (N,6-furfurftrylamaiinopurine) itself in a kinin-requiring strain of Clostridiumiii theermoccillum (20) raise the possibility that the relatively high BA requirement exhibited by the tissue cultuires used in this study is relate(d to their ability to degrade much of the kinin taken up. Presumably then, only a small amount of the BA entering soybean and tobacco tissue is actually functionial, perhaps as a part of soluble RNA.…”

Section: Methodsmentioning

confidence: 98%

Incorporation of a Kinin, n, 6-Benzyladenine into Soluble RNA

Fox

1966

Plant Physiol.

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“…Bioassays of the UV-absorbing bands in the chromatograms in step 9 before it was autoclaved at 1200, the other was filter sterilized and added after the medium had been autoclaved and allowed to cool to near the gelation temperature. The results for fraction II are shown in Figure 2 together with a kinetin control series plotted at half the concentration.…”

mentioning

confidence: 99%

“…Cytokinin activity, expressed as kinetin equivalents (KE), was bioassayed with tobacco callus cultures by standard procedures.8' 9 In most experiments UV absorption of solutions or chromatographic bands was used as far as possible to trace the active fractions.…”

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confidence: 99%

Isolation of cytokinins from Corynebacterium fascians.

Klämbt¹,

Thies²,

Skoog³

1966

Proc. Natl. Acad. Sci. U.S.A.

108

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Corynebacterium fascians derives its name from the characteristic symptoms of disease which infections by this organism cause in plants. '-4 These symptoms can be imitated by applications of kinetin, alone or in combinations with auxin, to seedlings,5 and the bacterium is known to produce cytokinin, as measured by release of bud inhibition or the retention of chlorophyll in excised tissues.6This report is on the isolation of cytokinins from liquid cultures of C. Purification Procedures and Results.-Preliminary tests showed that the bacterial suspensions or crude extracts were toxic to the tobacco tissue and could not be bioassayed for cytokinin activity without prior treatment. Bacterial suspensions, therefore, were filtered through Celite. The filter cake was lyophylized and extracted with about 10OX its volume of chloroform. The filtrate was passed through a Dowex-50 column (described in Fig. 1) which was thoroughly washed with water and then eluted with 5 N NH40H. Both the chloroform extract and the eluate were evaporated to dryness, and each was dissolved in a volume of water equal to one tenth the bacterial suspension. Results of one such experiment (Table 1) show a total activity of ca. 500,ug/liter KE which was about equally distributed between the cells and medium, but some toxic material was still present. Other 52

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Tests for Kinetin‐Like Growth Promoting Activities of Triacanthine and Its Isomer, 6‐(γ,γ‐Dimethylallylamino)‐Purine

Cited by 59 publications

References 8 publications

Control of Logarithmic Growth Rates of Tobacco Callus Tissue by Cytokinins

Control of Logarithmic Growth Rates of Tobacco Callus Tissue by Cytokinins

Incorporation of a Kinin, n, 6-Benzyladenine into Soluble RNA

Isolation of cytokinins from Corynebacterium fascians.

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