Incorporation of a Kinin, n, 6-Benzyladenine into Soluble RNA

Fox, Joan E.B.

doi:10.1104/pp.41.1.75

Cited by 86 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is consistent with the ez of Fox (13,14) and conclusively rules out any me volving transfer of the N5-sidechains of free c adenosine residues in RNA. The extent of incorr found to be extremely low, about 1 bzl'A mole tRNA molecules.…”

Section: Resultssupporting

confidence: 79%

“…Of the specific tRNAs that have been studied, only those which read a codon on the mRNA beginning with U (uridine) contain a cytokinin, and in those tRNAs which have been sequenced, the cytokinin is located adjacent to the anticodon on the 3'-side (24,29,30,34). There (28), van Onckelen et al (32), and Fox et al (13,14) reported the incorporation of cytokinins into sRNA although the purity of some of the sRNA was later questioned by Key (19). Dyson (8) and Srivastava (31) into both tRNA and rRNA, whereas Hall (16), Richmond et al (26), and Bezemer-Sybrandy et al (3) failed to detect any incorporation into RNA.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

et al. 1974

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 79%

mentioning

confidence: 99%

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

et al. 1974

View full text Add to dashboard Cite

show abstract

“…It has been shown that cytokinin-8-'4C supplied to plant tissues is extensively degraded (7,18). Recently, the short term metabolism of 6-benzylaminopurine (benzyladenine) in soybean tissue culture (6) or potato tuber slices (4) has been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Kinetin and Calcium in Relation to Their Effect on Stimulation of Ethylene Production

Lau¹,

Yang²

1975

Plant Physiol.

View full text Add to dashboard Cite

Application of kinetin and Ca2+ caused a striking synergistic increase in ethylene production by mung bean (Phaseolus aureus Roxb) hypocotyl segments. The effect of kinetin on Ca2+ uptake and of Ca'+ on the uptake and metabolism of kinetin in relation to their effect on ethylene production was studied. Tracer experiments showed that kinetin greatly increased the uptake of 4"Ca2' after 6 hours of incubation. Reciprocallv, Ca2+ stimulated the uptake of kinetin-8-14C and remarkably enhanced the metabolism of kinetin-8-14C into several polar metabolites. Consequently, the quantity of free kinetin-8-'4C remaining in Ca2+-treated segments was much less than in control segments. A possible mechanism accounting for the synergism between kinetin and calcium on ethylene production is discussed.We have previously reported a large synergistic increase in ethylene production by mung bean (Phaseoluts aureus Roxb) hypocotyl segments when kinetin and Ca2+ were applied (12). The amount of ethylene produced was dependent on the concentrations of kinetin and Ca2+ ion applied. Analysis of the synergistic effect of kinetin and Ca2+ on ethylene production required examination of the effect of kinetin on the uptake of Ca>2 and the reciprocal effect of Ca+ on the uptake and metabolism of kinetin. This paper describes the stimulatory effect of kinetin on Ca2+ uptake and of Ca>2 on kinetin uptake and metabolism. A possible mechanism accounting for the synergism between kinetin and Ca2+ ion on ethylene production is discussed. MATERIALS AND METHODSSeeds of mung bean (Phaseolus aureus Roxb) were grown in vermiculite for 3.5 days in darkness at 24 C. Segments 2 cm long were cut from hypocotyls at a point 1 cm below the hook, as previously described (11). Lots of 20 segments were incubated in 5 ml of a medium consisting of 50 mM potassium phosphate buffer (pH 6), 2% sucrose, and a given concentration of labeled and unlabeled kinetin or Ca2+, as indicated, in a 50-ml Erlenmeyer flask. A plastic centerwell containing 0.2 ml of 40% KOH was hung in the flask to absorb the CO2 evolved. The flasks were sealed with rubber serum caps and incubated in a shaker at 27 C in darkness.At the time intervals indicated, 1 -ml gas samples were 1 This work was supported by National Science Foundation Grant GB-33907X.withdrawn by hypodermic syringe, and ethylene was assayed with a gas chromatograph equipped with an alumina column and a flame ionization detector. The flask was then flushed with air and recapped for the next determination.For uptake studies, the hypocotyls incubated with 4"Ca'+ (104 /Ci, 41.5 ,umoles) were washed with five changes of distilled H20. The tissue was then ground in a glass homogenizer in 4 ml of 95% ethanol. The debris was pelleted by centrifugation and the supernatant was collected. The pellet was serially extracted with 2 ml of H20; 2 ml, 3 ml, and 5 ml of 10 mM HCl; and lastly with 5 ml of 0.5 mM EDTA. The radioactivity of the incubation medium, of each extract, and of the debris was determined with a liquid scintillation ...

show abstract

“…As one possibility, it was suggested that the incorporation of benzyladenine into sRNA may confer amino acid transfer activity to certaini tRNAs (7). Regulation at the level of amino acid transfer is well known in microorganisnms (27) …”

mentioning

confidence: 99%

“…Recently, evidence was presented that soybean and tobacco callus cultures, which require exogenous cytokinins for growth, incorporate one such growth regulator, 6-benzylaminopurine (benzyladenine), into sRNA (7). Chromatography of the labeled sRNA on O-benzoyldiethylaminoethyl celilulose showed that only 1 of the 6 fractions obtained wvas radioactive, and the bulk of this radioactivity appeared to be associated witlh the nucleotide of benzyladenine (8).…”

mentioning

confidence: 99%

On the Significance of Cytokinin Incorporation into RNA

Kende

Tavares

1968

Plant Physiol.

View full text Add to dashboard Cite

Abstract. The clarification of the following 2 questions was attempted: (a) are cytokinins precursors in the formation of sRNA, (b) is the observed incorporation of cytokinins into sRNA related to the action of the hormone? Although Escherichia coli contains cytokinins in its sRNA, no oytokinin auxotroph mutants of E. coli could be found and the statistical probabili,ty for the existence of such mutants is extremely low. This suggests that cytokinins are not preoursors in the synthesis of sRNA. A radioactive cytokinin, 6-benzylamino-9-methylpurine was synthesized and it was tested wheth-er or not it is incorporated into sRNA of soybean callus tissue. Masking the 9-position of the purine inhibited the incorporation of this cytokinin into RNA while not affecting its biological activity. This is taken as an indication that the observed incorporation of cytokinins such as benzyladenine into sRNA is not related to the action of this hormone.Substanices witlh cvtokinin activitv have been isolated fromi the hyvdrolvsates of the soluble RNA (sRNA) of microorganismiis, animals and plants (1,3,12,13,20,24,26). The minor base 6-.(y,y-di-

show abstract

Incorporation of a Kinin, n, 6-Benzyladenine into Soluble RNA

Cited by 86 publications

References 20 publications

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

Interaction of Kinetin and Calcium in Relation to Their Effect on Stimulation of Ethylene Production

On the Significance of Cytokinin Incorporation into RNA

Contact Info

Product

Resources

About