Corynebacterium fascians derives its name from the characteristic symptoms of disease which infections by this organism cause in plants. '-4 These symptoms can be imitated by applications of kinetin, alone or in combinations with auxin, to seedlings,5 and the bacterium is known to produce cytokinin, as measured by release of bud inhibition or the retention of chlorophyll in excised tissues.6This report is on the isolation of cytokinins from liquid cultures of C. Purification Procedures and Results.-Preliminary tests showed that the bacterial suspensions or crude extracts were toxic to the tobacco tissue and could not be bioassayed for cytokinin activity without prior treatment. Bacterial suspensions, therefore, were filtered through Celite. The filter cake was lyophylized and extracted with about 10OX its volume of chloroform. The filtrate was passed through a Dowex-50 column (described in Fig. 1) which was thoroughly washed with water and then eluted with 5 N NH40H. Both the chloroform extract and the eluate were evaporated to dryness, and each was dissolved in a volume of water equal to one tenth the bacterial suspension. Results of one such experiment (Table 1) show a total activity of ca. 500,ug/liter KE which was about equally distributed between the cells and medium, but some toxic material was still present. Other 52
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