Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test

Liman, Recep; Akyıl, Dilek; Eren, Yasin; Konuk, Muhsin

doi:10.1016/j.chemosphere.2010.05.011

Cited by 67 publications

(36 citation statements)

References 39 publications

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“…The phases of mitotic division may be affected the accumulation of the pesticides in the cell. The results of this study conformed with many earlier studies (Pandey 2008;Liman et al 2010;Yıldız and Arıkan 2008;Srivastava and Mishra 2009;Andrioli et al 2012).…”

Section: Discussionsupporting

confidence: 80%

“…Darlington and Mc-Leish (1951) proposed that stickiness might be due to degradation or depolymerization of chromosomal DNA. Besides, many researchers reported that its occurrences during the study could be caused of subchromatid linkage between chromosomes (Chauhan et al 1986;Ajay and Sarbhoy 1988;Kovalchuk et al 1998;Liman et al 2010). The bridges involving one or more chromosomes were the most important and frequent type in addition to sticky chromosomes.…”

Section: Discussionmentioning

confidence: 99%

“…have proven to be useful when used as bioindicators (Kluge and Podlesak 1985;Ma et al 1995;Amer et al 1999;Sang and Li 2004;Majer et al 2005;Mišik et al 2006;Gadeva and Dimitrov 2008;Enan 2009;Ö zkara et al 2011). Among them, Allium test is one of the well-known and reliable test systems to determine the toxicity in the laboratories (Fiskesjo 1985;Saxena et al 2005;Konuk et al 2007;Liman et al 2010). Onions are easy to store and to handle, and also macroscopic and microscopic parameters can be observed easily.…”

Section: Introductionmentioning

confidence: 99%

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Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…In the determination of application doses, 2xEC50, EC50 and 0.5x EC5, positive (MMS, 10 ppm) and negative control group were used for 12, 24, 48, 72 and 96 h. Fixation and staining of the root tip cells were carried out as reported earlier (Yıldız et al, 2009;Liman et al, 2010). The MI and the frequencies of chromosomal aberrations (CA) were carried out according to Saxena et al (2005).…”

Section: Allium Cepa Anaphase-telophase Testmentioning

confidence: 99%

“…Examining the mutagenicity of plant extract or chemical(s), Ames test, or the so-called Salmonella/ microsome test, is widely used (Konuk et al, 2008;Uysal et al, 2010;Liman et al, 2010). This test can be carried out rapidly and cheaply and it is also one of the most reliable short-term bacterial test systems (Maron & Ames, 1983;Mortelmans & Zeiger, 2000).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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