Testing culture media for pollen germination ofElaeis guineensisJacq. (oil palm, Arecaceae)

Abstract

Understanding pollen germination is of great importance for breeding and conservation. We present a protocol for evaluating culture media for germination of pollen of Elaeis guineensis. Samples were collected in the municipality of Nilo Pec ßanha (State of Bahia, Brazil). A solution of aniline blue in lactophenol was used to determine viability of pollen grains. Two alternative culture media to evaluate germination rates of pollen grains were tested: M1, Brewbaker and Kwack medium; and M2, BCa medium, with addition of boron and calcium. Both media were used with 20% sucrose at pH 6.5. The pollen grains were dried at room temperature in silica gel for 4 h and later placed on slides containing culture media, kept in a Petri dish with filter paper wetted with distilled water and stored in chamber at 30°C for 24 h. The greatest viability rate was 94% for fresh pollen grains. Highest germination rates were 26 and 64% for M1 and M2, respectively. There was no positive correlation between viability and germination for either M1 or M2 (Pearson's and Spearman's correlation, respectively). Dried pollen on BCa medium (M2) is useful for in vitro germination experiments of E. guineensis given appropriate concentration of sucrose and ions, pH, temperature and incubation time and in the absence of light.

In vitro viability of pollen grain of fiji dwarf coconut accession

“…However, at the other incubation temperature levels (22, 25 and 31 °C) the Armendariz culture medium showed higher in vitro pollen germination percentages ranged from 33.3 to 34.46% than the Lora culture medium. In contrast, Sousa et al (2016) no observed positive correlation between viability and germination for different culture media on the pollen germination of oil palm (Elaeis guineensis Jacq).…”

“…In addition, lack of sucrose has been shown to result in damage to pollen grains under in vitro conditions (Baloch et al, 2001). Sugars play a fundamental role in the culture medium and several studies point to sucrose as a stimulating agent for pollen germination (Sousa;Schemberg;Aguiar, 2010). Dafni (1992) points out that the ideal concentrations of sucrose in germination tests for the different pollen types should be in the range of 0 and 500 g L -1 .…”

Morphology, viability, and longevity of pollen of National Type and Trinitarian (CCN-51) clones of cocoa (Theobroma cacao L.) on the Coast of Ecuador

“…To calculate the in vivo germination, manual pollination was performed using fresh pollen from co-specific individuals (12 clones, 3 ramets per clone, and 10 flowers per ramet), it is proven that higher germination rates occur while using fresh pollen grains (Cohen 2008;Sousa et al 2016). Floral buds were previously isolated before anthesis to ensure that the stigmas receive no pollen from nearby individuals.…”

Oil palm crop: state and gaps of research and technological development at global scale, Latin America and Mexico

“…Recently, environmental studies have increased in this region (19.7%), and some research subjects have been based on OP production and the challenge of including the agroecology concept for environmental impact reduction, not only for OP used as food but also as bioenergy feedstock (Castellanos-Navarrete and Jansen, 2017). Other research themes focused on the impacts and interactions, such as the expansion of OP and its social and economic effects (14.1%) (Bennett et al, 2018;Gonzalez-Salazar et al, 2017), the impact of fertilization (8.1%) (Bastos de Matos et al, 2017) and genetic improvements (7.0%) (De Farias Neto et al, 2013;Moreno-Caicedo and Bastidas-Pérez, 2017;Sousa et al, 2016), the response of physiological parameters to abiotic factors (gas exchange, water use, and foliar area) (3.5%) ( Murugesan et al, 2017;Rivera Mendez et al, 2012), the effect of irrigation and drainage (1.7%), yield of FFB (0.2%) (Contreras et al, 2012;De Farias Neto et al, 2013), the use of OP as a bioenergy feedstock (7.6%) (Aristizabal et al, 2016;Cardoso et al, 2017), and the impacts on biodiversity (2.6%) (Alonso-Rodriguez et al, 2017; Knowlton et al, 2017) and ecosystem services (2.0%) (Frazão et al, 2013;Lavelle et al, 2014). Other studies also focused on the impact of palm oil on human consumption by characterizing oil products and health conditions (3.8%) (Irias-Mata et al, 2017, Sandoval-Garcia et al, 2016) (Fig.…”