Testicular steroidogenesis in adult men with human chorionic gonadotropin-producing tumors.

Rieu, M; Reznik, Yves; Vannetzel, Jean-Michel; Mahoudeau, J; Berrod, Jean-Louis; Kuhn, Jean-Marc

doi:10.1210/jcem.80.8.7629236

Cited by 8 publications

(11 citation statements)

References 28 publications

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“…The data in infertile men are numerous [3,14,19; for reviews, see 2,35], and several studies have focused on the understanding of human testicular cell functions in vitro [1,17,28,36,40; for review, see 7]. Besides the special architecture of the Sertoli cells [10,43], the presence of integrins and cadherins, which are involved in the cell-cell adhesion, has been reported [31].…”

mentioning

confidence: 99%

Increased Testicular Steroid Concentrations in Patients With Idiopathic Infertility and Normal FSH Levels

Marie¹,

Galeraud-Denis²,

Carreau³

2001

Archives of Andrology

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confidence: 99%

Increased Testicular Steroid Concentrations in Patients With Idiopathic Infertility and Normal FSH Levels

Marie¹,

Galeraud-Denis²,

Carreau³

2001

Archives of Andrology

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“…In contrast, high LH levels induced only slightly decreasing androstenedione levels which remained higher than those in patients with normal LH levels. 17-hydroxylase activity, irrespective of LH levels. This strongly suggests that serum factors induce these P450 activity changes in most of these patients.…”

mentioning

confidence: 85%

“…The detection limit was 0.1 IU/l. Serum 17-OHP, androstenedione and testosterone levels were measured by RIA after chromatography on a Celite column (Touzet Matignon, Paris, France) and serum E 2 was determined by RIA, as previously described (17). For all these steroid hormones, intra-and interassay coefficients of variation were less than 6% and less than 9% respectively.…”

Section: Assaysmentioning

confidence: 99%

“…These LH-induced down-regulating effects appear to be more marked on 17,20-lyase than on 17-hydroxylase (12)(13)(14)(15). In vivo testicular P450 downregulation by very high serum human chorionic gonadotropin (hCG) concentrations has also been reported both in normal men (16) and in men with hCG-producing tumors (17). In vivo LH-induced ovarian D 4 P450 down-regulation has not been reported in women.…”

Section: Introductionmentioning

confidence: 99%

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High serum luteinizing hormone levels induce ovarian delta4 cytochrome P450c17alpha down-regulation in hirsute women: complete effect on 17-hydroxylase and partial effect on 17,20-lyase

Rieu

Mourrieras²,

Riveline³

et al. 1998

European Journal of Endocrinology

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It is well known that normal and mildly elevated luteinizing hormone (LH) levels induce increased activity of ovarian 17-hydroxylase and 17,20-lyase, the cytochrome P450c17a (P450) enzymes. This leads to increased ovarian 17a-hydroxyprogesterone (17-OHP) and androstenedione production. In contrast, it has been shown in both in vitro and in vivo studies in animals and in in vitro studies in women that high LH concentrations have opposite effects on these enzymes. These LH downregulating effects appear to be more marked on 17,20-lyase than on 17-hydroxylase. Finally, these LH effects have not been reported in vivo in women. Therefore, we investigated the relationships between serum LH levels and serum 17-OHP and androstenedione concentrations in 263 consecutive hirsute women (HW) with normal serum 17-OHP responses to acute adrenocorticotropin (ACTH) stimulation. The patterns of basal serum steroid concentrations differed according to the basal serum LH levels. Indeed, for relationships between LH and 17-OHP concentrations, a positive correlation (P < 0.001) was found between the levels of these parameters when LH levels ranged from 0.2 to 9.0 IU/l. Conversely, for LH levels greater than 9.0 to 21.0 IU/l, LH values were negatively correlated (P < 0.001) with 17-OHP concentrations. Similar results were observed for relationships between LH and androstenedione levels but the LH peak level related to decreasing androstenedione concentrations was 12.0 IU/l. Finally, the mean 17-OHP level in patients with LH levels which induced marked P450 down-regulation (i.e. more than 12 IU/l) was similar to that in patients with LH levels within the normal range (i.e. less than 6 IU/l). In contrast, the mean androstenedione level in the former patients was markedly higher (P < 0.001) than that in the latter patients. In conclusion, as previously reported in in vitro sudies, this in vivo study indicates that LH induces stimulating and downregulating effects on both ovarian D 17-hydroxylase down-regulation in these patients.

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“…rhLH doses were injected intravenously in a random order. Then, the blood was withdrawn at the following times (30,60,90,120,150,180,240, and 300 min) in order to measure LH, T, and E 2 plasma levels.…”

Section: Subjects Studied and Protocolsmentioning

confidence: 99%

Evaluation of endocrine testing of Leydig cell function using extractive and recombinant human chorionic gonadotropin and different doses of recombinant human LH in normal men

Cailleux-Bounacer

Reznik

Cauliez

et al. 2008

European Journal of Endocrinology

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Background: The functional testing of endocrine testis uses extractive human chorionic gonadotropin (ehCG). Recombinant human hCG (rhCG), avoiding any contamination, should replace ehCG. Moreover, a functional evaluation with recombinant human LH (rhLH) would be closer to physiology than a pharmacological testing with hCG. Methods: The study was conducted in normal men. We first evaluated the dose-effect of ehCG on plasma testosterone and estradiol levels, before and after injection of either hCG or vehicle. Secondly, the responses to the optimal dose of ehCG were compared with those of rhCG. Thirdly, we investigated the dose-effect of rhLH, on steroid hormone secretion. LH, testosterone, and estradiol plasma levels were measured after the injection of either rhLH or placebo. Results: ehCG induced dose-dependent increases in plasma estradiol and testosterone levels. They respectively peaked at 24 and 72 h after the injection. The most potent dose of ehCG (5000 IU) induced results similar to those observed with 250 mg (6500 IU) rhCG. By comparison with placebo, rhLH induced a significant and dose-dependent increase in plasma testosterone levels 4 h after the injection. Peak response of testosterone to rhLH and rhCG was significantly correlated. rhLH did not induce significant change in plasma estradiol level. Conclusions: In normal men, a single i.v. injection of 150 IU rhLH induces a 25% rise in plasma testosterone levels by comparison with placebo. At the moment, the dynamic evaluation using hCG remains the gold standard test to explore the Leydig cell function. The use of 250 mg rhCG avoiding any contamination should be recommended.

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Testicular steroidogenesis in adult men with human chorionic gonadotropin-producing tumors.

Cited by 8 publications

References 28 publications

Increased Testicular Steroid Concentrations in Patients With Idiopathic Infertility and Normal FSH Levels

Increased Testicular Steroid Concentrations in Patients With Idiopathic Infertility and Normal FSH Levels

High serum luteinizing hormone levels induce ovarian delta4 cytochrome P450c17alpha down-regulation in hirsute women: complete effect on 17-hydroxylase and partial effect on 17,20-lyase

Evaluation of endocrine testing of Leydig cell function using extractive and recombinant human chorionic gonadotropin and different doses of recombinant human LH in normal men

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