Tenacibaculum jejuense sp. nov., isolated from coastal seawater

Abstract

A Gram-staining-negative, strictly aerobic and rod-shaped bacterium, designated strain CNURIC013 T , was isolated from seawater collected on the coast of Jeju Island, South Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CNURIC013 T belonged to the genus Tenacibaculum, within the family Flavobacteriaceae. Sequence similarities between the novel strain and the type strains of recognized species of the genus Tenacibaculum were 93.6-96.0 %, the highest value being with Tenacibaculum litopenaei B-I T (96 %). The DNA G+C content of the novel strain was 34.5 mol% and the major respiratory quinone was menaquinone-6. The major fatty acids were summed feature 3 (comprising C 16 : 1 v7c and/or iso-C 15 : 0 2-OH; 26.0 %), iso-C 15 : 0 (24.4 %), iso-C 15 : 1 G (18.5 %) and iso-C 17 : 0 3-OH (8.1 %). The polar lipids consisted of phosphatidylethanolamine, one unknown aminophospholipid and nine unknown polar lipids. On the basis of the phenotypic, phylogenetic and genotypic data, strain CNURIC013 T represents a novel species within the genus Tenacibaculum, for which the name Tenacibaculum jejuense sp. nov. is proposed. The type strain is CNURIC013 T (5KCTC 22618 T 5JCM 15975 T ).The genus Tenacibaculum was first proposed by Suzuki et al. (2001), who described Tenacibaculum mesophilum and Tenacibaculum amylolyticum and reclassified Flexibacter maritimus (Wakabayashi et al., 1986) and Flexibacter ovolyticus (Hansen et al., 1992) as Tenacibaculum maritimum and Tenacibaculum ovolyticum, respectively. The genus Tenacibaculum belongs to the family Flavobacteriaceae and currently comprises 15 recognized species isolated from different marine habitats. T. maritimum, T. ovolyticum, Tenacibaculum discolor, Tenacibaculum aiptasiae and Tenacibaculum soleae were isolated from diseased marine animals (Wakabayashi et al., 1986;Hansen et al., 1992;Wang et al., 2008; Piñeiro-Vidal et al., 2008a, b). Although T. mesophilum, Tenacibaculum adriaticum and Tenacibaculum crassostreae were also isolated from marine animals (Suzuki et al., 2001;Heindl et al., 2008;Lee et al., 2009), T. amylolyticum was isolated from marine macroalgae (Suzuki et al., 2001), Tenacibaculum lutimaris, Tenacibaculum litoreum and Tenacibaculum aestuarii were isolated from tidal-flat sediments (Yoon et al., 2005;Choi et al., 2006;Jung et al., 2006) and Tenacibaculum skagerrakense, Tenacibaculum litopenaei and Tenacibaculum gallaicum were isolated from seawater (Frette et al., 2004;Sheu et al., 2007; Piñeiro-Vidal et al., 2008a). In this study, a Tenacibaculumlike bacterial strain, CNURIC013 T , was isolated from a seawater sample collected from the coast of Jeju Island, South Korea, before its taxonomy was investigated using a polyphasic approach. Strain CNURIC013 T was isolated, using a standard dilutionplating technique, on marine agar 2216 (MA; Difco), after incubation at 25 u C for 5 days. Subcultivation was routinely performed on MA at 30 u C for 3 days under aerobic conditions and the strain was preserved at 280 u C in marine broth (M...

Multilocus Sequence Analysis of the Marine Bacterial Genus Tenacibaculum Suggests Parallel Evolution of Fish Pathogenicity and Endemic Colonization of Aquaculture Systems

“…Tenacibaculum strains not apparently associated with fish diseases also are widespread in marine environments, where they may decompose organic matter, as suggested by their ability to degrade a variety of biopolymers, such as various cellulose derivatives, xylan, agar, and chitin (2,(13)(14)(15)(16). T. mesophilum, T. amylolyticum, T. aiptasiae, T. adriaticum, T. crassostreae, and "T. halocynthiae" (quotation marks denote names that have not been validly published) were isolated from marine organisms (2,15,(17)(18)(19).…”

“…Also belonging to this category is T. litopenaei, a chitinolytic bacterium isolated from the water of a shrimp mariculture pond (20). Other Tenacibaculum species were retrieved from inorganic substrates; T. litoreum, T. lutimaris, T. aestuarii, and T. caenipelagi were isolated from tidal flat sediments (14,(21)(22)(23), while T. skagerrakense, T. jejuense, T. geojense, and T. xiamenense were isolated from seawater at various distances from the shore and positions in the water column (13,16,24,25).…”

Tenacibaculosis in aquaculture farmed marine fish

“…The first report of the bacterium in Europe was made in 1982, in Dover sole (Solea solea) (Campbell and Buswell, 1982;Bernardet et al, 1990 lutimaris were transferred to the new genus Tenacibaculum (Suzuki et al, 2001), based on the nucleotide sequence of the gene gyrB. The genus Tenacibaculum (Suzuki et al, 2001), belongs to the family Flavobacteriaceae and today includes the species: T. adriaticum (marine bryozoan), T. aestuarii (tidal flat sediment), T. aiptasiae (sea anemone), T. amylolyticum (marine macroalgae), T. crassostreae (pacific oyster), T. dicentrarchi (European sea bass), T. discolor (Dover sole), T. gallaicum (turbot), T. japonica, T. litopenaei (shrimp mariculture pond), T. litoreum (tidal flat sediment), Tenacibaculum lutimaris (tidal flat sediment), Tenacibaculum maritimun (diseased marine fish), T. mesophilum (sponges), T. ovolyticum (fish eggs), T. skagerrakense (seawater), T. soleae (Dover sole) T. jejuense (offshore seawater) (Wakabayashi et al,1986;Hansen et al, 1992;Suzuki et al, 2001;Frette et al, 2004;Yoon et al, 2005;Choi et al, 2006;Jung et al, 2006;Sheu et al, 2007;Heindl et al, 2008;Wang et al, 2008;Piñeiro-Vidal et al, 2008a,b;Lee et al, 2009;Oh et al, 2012;Piñeiro-Vidal et al, 2012). (Baxa et al, 1986;Wakabayashi et al,1986;Bernardet et al, 1990;Chen et al, 1995;Soltani et al,1996;Santos et al, 1999;Cepeda and Santos, 2002;Salati et al, 2005;Toranzo et al, 2005;Avendaño-Herrera et al, 2004b,2005bMagi et al, 2006;van Gelderen et al, 2009).…”

“…Among the cultured fish, the disease has been reported in marine fish species, like red sea bream (Pagrus major), gilthead seabream (Sparus aurata),european sea bass (Dincentrarchus labrax), atlantic salmon (Salmo salar), sole (Solea solea), turbot (Scophthalmus maximus) and many other cultured and wild fish in Europe, Japan, North America, Australia and all over the world White, 1979,1982;Wakabayashi et al, 1986;Devesa et al, 1989;Pazos et al, 1993;Chen et al, 1995;Handlinger et al, 1997;Ostland et al, 1999;Santos et al, 1999;Avendaño-Herrera et al, 2004a, Choi et al, 2006Jung et al, 2006;Sheu et al, 2007;Vatsos, 2007;Heindl et al, 2008;Wang et al, 2008;Piñeiro-Vidal et al, 2008a,b;Lee et al, 2009;Oh et al, 2012;Piñeiro-Vidal et al, 2012;Failde et al, 2013).…”

Tenacibaculum sediminilitoris sp. nov., isolated from a tidal flat

“…html; Parte, 2014;Kim et al, 2016). Members of the genus Tenacibaculum have been isolated from a variety of marine environments and marine organisms (Suzuki et al, 2001;Frette et al, 2004;Yoon et al, 2005;Sheu et al, 2007;Heindl et al, 2008;Piñeiro-Vidal et al, 2008Wang et al, 2008;Lee et al, 2009;Oh et al, 2012;Li et al, 2013;Kim et al, 2013Kim et al, , 2016. The aim of the present work was to determine the exact taxonomic position of strain YKTF-3 T by using a polyphasic characterization.…”