Temporal transcriptomic response during arsenic stress in Herminiimonas arsenicoxydans

Cleiss-Arnold, Jessica; Koechler, Sandrine; Proux, Caroline; Fardeau, Marie‐Laure; Dillies, Marie‐Agnès; Coppée, Jean‐Yves; Arsène-Ploetze, Florence; Bertin, Philippe

doi:10.1186/1471-2164-11-709

Cited by 69 publications

(71 citation statements)

References 34 publications

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“…Arsenite early exposure (15 minutes) induced the transcription of two usp genes in Herminiimonas arsenicoxydans , a bacterium isolated from arsenic-contaminated sludge 40. A usp gene and an arsenic resistance operon are located on an antibiotic-resistant island in the genome of Acinetobacter baumannii , an opportunistic pathogen that causes nosocomial infections 41.…”

Section: Discussionmentioning

confidence: 99%

Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

et al. 2014

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The influence of environmental chemicals including arsenic, a type 1 carcinogen, on the composition and function of the human-associated microbiota is of significance in human health and disease. We have developed a suite of bioinformatics and visual analytics methods to evaluate the availability (presence or absence) and abundance of functional annotations in a microbial genome for seven Pfam protein families: As(III)-responsive transcriptional repressor (ArsR), anion-transporting ATPase (ArsA), arsenical pump membrane protein (ArsB), arsenate reductase (ArsC), arsenical resistance operon transacting repressor (ArsD), water/glycerol transport protein (aquaporins), and universal stress protein (USP). These genes encode function for sensing and/or regulating arsenic content in the bacterial cell. The evaluative profiling strategy was applied to 3,274 genomes from which 62 genomes from 18 genera were identified to contain genes for the seven protein families. Our list included 12 genomes in the Human Microbiome Project (HMP) from the following genera: Citrobacter, Escherichia, Lactobacillus, Providencia, Rhodococcus, and Staphylococcus. Gene neighborhood analysis of the arsenic resistance operon in the genome of Bacteroides thetaiotaomicron VPI-5482, a human gut symbiont, revealed the adjacent arrangement of genes for arsenite binding/transfer (ArsD) and cytochrome c biosynthesis (DsbD_2). Visual analytics facilitated evaluation of protein annotations in 367 genomes in the phylum Bacteroidetes identified multiple genomes in which genes for ArsD and DsbD_2 were adjacently arranged. Cytochrome c, produced by a posttranslational process, consists of heme-containing proteins important for cellular energy production and signaling. Further research is desired to elucidate arsenic resistance and arsenic-mediated cellular energy production in the Bacteroidetes.

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Section: Discussionmentioning

confidence: 99%

Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

et al. 2014

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“…It is not immediately apparent why, or how, such a transporter might be involved (or not) in As III oxidation or metabolism. Previously reported microarray studies also identified the As III induction of a C 4 -dicarboxylate transporter in H. arsenicoxydans (8). The Dct system in strain 5A is a secondary transporter operating without a periplasmic solute binding protein, whereas the As III -inducible dctPMQ dicarboxylate transporter in H. arsenicoxydans is annotated as a tripartite ATP-independent periplasmic (TRAP)-type transporter (reviewed in reference 45).…”

Section: Discussionmentioning

confidence: 99%

Involvement of RpoN in Regulating Bacterial Arsenite Oxidation

Kang

Bothner

Rensing

et al. 2012

Appl Environ Microbiol

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In this study with the model organism Agrobacterium tumefaciens, we used a combination of lacZ gene fusions, reverse transcriptase PCR (RT-PCR), and deletion and insertional inactivation mutations to show unambiguously that the alternative sigma factor RpoN participates in the regulation of As III oxidation. A deletion mutation that removed the RpoN binding site from the aioBA promoter and an aacC3 (gentamicin resistance) cassette insertional inactivation of the rpoN coding region eliminated aioBA expression and As III oxidation, although rpoN expression was not related to cell exposure to As III . Putative RpoN binding sites were identified throughout the genome and, as examples, included promoters for aioB, phoB1, pstS1, dctA, glnA, glnB, and flgB that were examined by using qualitative RT-PCR and lacZ reporter fusions to assess the relative contribution of RpoN to their transcription. The expressions of aioB and dctA in the wild-type strain were considerably enhanced in cells exposed to As III , and both genes were silent in the rpoN::aacC3 mutant regardless of As III . The expression level of glnA was not influenced by As III but was reduced (but not silent) in the rpoN::aacC3 mutant and further reduced in the mutant under N starvation conditions. The rpoN::aacC3 mutation had no obvious effect on the expression of glnB, pstS1, phoB1, or flgB. These experiments provide definitive evidence to document the requirement of RpoN for As III oxidation but also illustrate that the presence of a consensus RpoN binding site does not necessarily link the associated gene with regulation by As III or by this sigma factor. E vidence of microbial arsenite (As III ) oxidation was first reported nearly a century ago (18). Subsequent progress has been sporadic, with work that identified some organisms capable of As III oxidation (46,48,60) and then a study of a Pseudomonas arsenitoxidans strain reported to grow chemolithoautotrophically with As III as a sole electron donor (23). Subsequent follow-up characterizations of this organism and this process failed to materialize; however, approximately 2 decades later, Santini et al. (52) described the isolation and initial characterization of a Rhizobiumlike bacterium (strain NT-26) that could grow chemolithoautotrophically with As III as a sole electron donor for energy generation and with CO 2 as a sole carbon source. Soon thereafter, and in part stimulated by the massive arsenic poisoning disaster in Bangladesh (2), a series of studies initiated the characterization of microbial As III oxidation in natural environments, including geothermal springs (9,11,12,17,19,24,25,35,51) and soils (41); in mining-contaminated environments (6, 13, 40); and, most recently, in anoxic photosynthesis (21, 33). Likewise, progress has been made in the understanding of the biochemistry of the As III oxidase enzyme (1,14,37 oxidase structural genes were later cloned from the above-mentioned Rhizobium NT-26 organism (53). The symbols for genes coding for functions associated with As III oxidation have...

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“…In comparative proteomic and genomic analyses in the pres- ence or absence of As(III), the enzymes involved in the tricarboxylic acid (TCA) cycle were upregulated after the addition of As(III), indicating the bacteria needed a large amount of energy to resist As(III) toxicity (140)(141)(142). In contrast, the TCA cycle in A. tumefaciens GW4 was downregulated by the addition of As(III).…”

Section: Metabolic Pathways Associated With Antimonite Resistancementioning

confidence: 99%

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Wang

Oremland

et al. 2016

Appl Environ Microbiol

161

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e Antimony (Sb) is a toxic metalloid that occurs widely at trace concentrations in soil, aquatic systems, and the atmosphere. Nowadays, with the development of its new industrial applications and the corresponding expansion of antimony mining activities, the phenomenon of antimony pollution has become an increasingly serious concern. In recent years, research interest in Sb has been growing and reflects a fundamental scientific concern regarding Sb in the environment. In this review, we summarize the recent research on bacterial antimony transformations, especially those regarding antimony uptake, efflux, antimonite oxidation, and antimonate reduction. We conclude that our current understanding of antimony biochemistry and biogeochemistry is roughly equivalent to where that of arsenic was some 20 years ago. This portends the possibility of future discoveries with regard to the ability of microorganisms to conserve energy for their growth from antimony redox reactions and the isolation of new species of "antimonotrophs."

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Temporal transcriptomic response during arsenic stress in Herminiimonas arsenicoxydans

Cited by 69 publications

References 34 publications

Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

Involvement of RpoN in Regulating Bacterial Arsenite Oxidation

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

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