Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

Isokpehi, Raphael D.; Udensi, Udensi K.; Simmons, Shaneka S.; Hollman, Antoinesha L.; Cain, Antia; Olofinsae, Samson A.; Hassan, Oluwabukola A.; Kashim, Zainab A.; Enejoh, Ojochenemi A.; Fasesan, Deborah E.; Nashiru, Oyekanmi

doi:10.4137/mbi.s18076

Cited by 26 publications

(24 citation statements)

References 49 publications

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“…Our collaboration with communities in Nigeria at risk of schistosomiasis bladder-related pathologies presents opportunities to conduct basic and translational research projects[ 6 , 9 , 17 ]. We are interested in understanding the influences of microbial taxa on human health and disease [ 18 ] including the induction of bladder pathologies in urogenital schistosomiasis. Genomic sequencing technologies can determine the membership of, and functions performed by microbial communities (microbiome) in the urine [ 19 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

The microbiome in urogenital schistosomiasis and induced bladder pathologies

et al. 2017

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BackgroundHuman schistosomiasis is a highly prevalent neglected tropical disease (NTD) caused by Schistosoma species. Research on the molecular mechanisms influencing the outcomes of bladder infection by Schistosoma haematobium is urgently needed to develop new diagnostics, therapeutics and infection prevention strategies. The objective of the research study was to determine the microbiome features and changes in urine during urogenital schistosomiasis and induced bladder pathologies.MethodologySeventy participants from Eggua, southwestern Nigeria provided morning urine samples and were screened for urogenital schistosomiasis infection and bladder pathologies in a cross-sectional study. Highthroughput NGS sequencing was carried out, targeting the 16S V3 region. Filtered reads were processed and analyzed in a bioinformatics pipeline.Principal findingsThe study participants (36 males and 34 females, between ages 15 and 65) were categorized into four groups according to status of schistosomiasis infection and bladder pathology. Data analytics of the next-generation sequencing reads revealed that Proteobacteria and Firmicutes dominated and had influence on microbiome structure of both non-infected persons and persons with urogenital schistosomiasis. Furthermore, gender and age influenced taxa abundance independent of infection or bladder pathology. Several taxa distinguished urogenital schistosomiasis induced bladder pathologies from urogenital schistosomiasis infection alone and from healthy persons, including known immune-stimulatory taxa such as Fusobacterium, Sphingobacterium and Enterococcus. Some of these significant taxa, especially Sphingobacterium were projected as markers of infection, while several genera including potentially beneficial taxa such as Trabulsiella and Weissella, were markers of the non-infected. Finally, expected changes in protein functional categories were observed to relate to cellular maintenance and lipid metabolism.ConclusionThe urinary microbiome is a factor to be considered in developing biomarkers, diagnostic tools, and new treatment for urogenital schistosomiasis and induced bladder pathologies.

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Section: Introductionmentioning

confidence: 99%

The microbiome in urogenital schistosomiasis and induced bladder pathologies

et al. 2017

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“…Gene selection ideally would encompass as many functions as possible (see Figs and ). Very few human microbiome relevant microorganisms carry a full suite of ars genes ( arsA , arsB , arsC , arsD , arsR , aqp and usp ) (Isokpehi et al ., ), but in reality an organism would only need an arsC and arsB/acr3 and perhaps a means of regulation (typically arsR ) in order to contribute to pre‐systemic host As metabolism.…”

Section: Arsenic and The Git Microbiomementioning

confidence: 99%

Arsenic and the gastrointestinal tract microbiome

McDermott

Stolz

Oremland

2020

Environ Microbiol Rep

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Summary Arsenic is a toxin, ranking first on the Agency for Toxic Substances and Disease Registry and the Environmental Protection Agency Priority List of Hazardous Substances. Chronic exposure increases the risk of a broad range of human illnesses, most notably cancer; however, there is significant variability in arsenic‐induced disease among exposed individuals. Human genetics is a known component, but it alone cannot account for the large inter‐individual variability in the presentation of arsenicosis symptoms. Each part of the gastrointestinal tract (GIT) may be considered as a unique environment with characteristic pH, oxygen concentration, and microbiome. Given the well‐established arsenic redox transformation activities of microorganisms, it is reasonable to imagine how the GIT microbiome composition variability among individuals could play a significant role in determining the fate, mobility and toxicity of arsenic, whether inhaled or ingested. This is a relatively new field of research that would benefit from early dialogue aimed at summarizing what is known and identifying reasonable research targets and concepts. Herein, we strive to initiate this dialogue by reviewing known aspects of microbe–arsenic interactions and placing it in the context of potential for influencing host exposure and health risks. We finish by considering future experimental approaches that might be of value.

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“…As metabolism by human gut microbiota was also explored. First, high-throughput sequencing data from the Human Microbiome Project (HMP) indicates that the genes related to As transportation and metabolism, such as Aqp, arsA, arsB, arsC and arsD, are widely distributed in the genomes of human gut bacteria [136]. Indeed, in vitro fermentation confirmed the As metabolizing ability of the human gut microbiota.…”

Section: Gut Microbiome and As Susceptibilitymentioning

confidence: 99%

Individual susceptibility to arsenic-induced diseases: the role of host genetics, nutritional status, and the gut microbiome

Chi

Gao

et al. 2018

Mamm Genome

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Arsenic (As) contamination in water or food is a global issue affecting hundreds of millions of people. Although As is classified as a group 1 carcinogen and is associated with multiple diseases, the individual susceptibility to As-related diseases is highly variable, such that a proportion of people exposed to As have higher risks of developing related disorders. Many factors have been found to be associated with As susceptibility. One of the main sources of the variability found in As susceptibility is the variation in the host genome, namely, polymorphisms of many genes involved in As transportation, biotransformation, oxidative stress response and DNA repair affect the susceptibility of an individual to As toxicity and then influence the disease outcomes. In addition, lifestyles and many nutritional factors, such as folate, vitamin C, and fruit, have been found to be associated with individual susceptibility to As-related diseases. Recently, the interactions between As exposure and the gut microbiome have been of particular concern. As exposure has been shown to perturb gut microbiome composition, and the gut microbiota has been shown to also influence As metabolism, which raises the question of whether the highly diverse gut microbiota contributes to As susceptibility. Here, we review the literature and summarize the factors, such as host genetics and nutritional status, that influence As susceptibility, and we also present potential mechanisms of how the gut microbiome may influence As metabolism and its toxic effects on the host to induce variations in As susceptibility. Challenges and future directions are also discussed to emphasize the importance of characterizing the specific role of these factors in inter-individual susceptibility to As-related diseases.

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Evaluative Profiling of Arsenic Sensing and Regulatory Systems in the Human Microbiome Project Genomes

Cited by 26 publications

References 49 publications

The microbiome in urogenital schistosomiasis and induced bladder pathologies

The microbiome in urogenital schistosomiasis and induced bladder pathologies

Arsenic and the gastrointestinal tract microbiome

Individual susceptibility to arsenic-induced diseases: the role of host genetics, nutritional status, and the gut microbiome

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