Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Li, Jingxin; Wang, Qian; Oremland, Ronald S.; Kulp, Thomas R.; Rensing, Christopher; Wang, Gejiao

doi:10.1128/aem.01375-16

Cited by 152 publications

(85 citation statements)

References 137 publications

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“…About 60 Sb(III)-oxidizing bacterial strains have been found and some of them can also oxidize arsenite [As(III)] to arsenate [As(V)] [ 14 ]. Recently, our group and collaborators demonstrated that the As(III) oxidase AioAB, which oxidizes the more toxic As(III) to the less toxic As(V) in the periplasm, could also catalyze Sb(III) oxidation in Agrobacterium tumefaciens 5A [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, global analysis of cellular responses to Sb(III) was performed using comparative proteomics with or without the addition of 50 μM Sb(III) in strain GW4 [ 16 ]. It was shown that Ars-resistance, Sb(III) oxidase AnoA, phosphate metabolism, carbohydrate metabolism, and energy generation were induced by Sb(III) [ 14 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Yang

Shi

et al. 2017

PLoS ONE

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Agrobacterium tumefaciens GW4 is a heterotrophic arsenite [As(III)]/antimonite [Sb(III)]-oxidizing strain. The As(III) oxidase AioAB is responsible for As(III) oxidation in the periplasm and it is also involved in Sb(III) oxidation in Agrobacterium tumefaciens 5A. In addition, Sb(III) oxidase AnoA and cellular H2O2 are also responsible for Sb(III) oxidation in strain GW4. However, the deletion of aioA increased the Sb(III) oxidation efficiency in strain GW4. In the present study, we found that the cell mobility to Sb(III), ATP and NADH contents and heat release were also increased by Sb(III) and more significantly in the aioA mutant. Proteomics and transcriptional analyses showed that proteins/genes involved in Sb(III) oxidation and resistance, stress responses, carbon metabolism, cell mobility, phosphonate and phosphinate metabolism, and amino acid and nucleotide metabolism were induced by Sb(III) and were more significantly induced in the aioA mutant. The results suggested that Sb(III) oxidation may produce energy. In addition, without periplasmic AioAB, more Sb(III) would enter bacterial cells, however, the cytoplasmic AnoA and the oxidative stress response proteins were significantly up-regulated, which may contribute to the increased Sb(III) oxidation efficiency. Moreover, the carbon metabolism was also activated to generate more energy against Sb(III) stress. The generated energy may be used in Sb transportation, DNA repair, amino acid synthesis, and cell mobility, and may be released in the form of heat.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Yang

Shi

et al. 2017

PLoS ONE

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“…Phosphorus is a critical nutrient for the growth of bacteria and is part of many biomolecules in Another potential environmental factor acting on soil E. coli in watershed with widespread produce fields identified in this study is antimony. Antimony is a toxic metalloid present widely at trace concentrations in natural soil (47,48). Its concentration could be elevated or even reach contamination threshold in agricultural lands due to human activities (49).…”

Section: Discussionmentioning

confidence: 99%

Adjacent terrestrial landscapes impact the biogeographical pattern of soil Escherichia coli in produce fields by modifying the importance of environmental selection and dispersal

Liao

Bergholz

2020

Preprint

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ABSTRACTHigh-quality habitats for wildlife (e.g., forest) provide essential ecosystem services while increasing species diversity and habitat connectivity. Unfortunately, presence of such habitats adjacent to produce fields may increase risk for contamination of fruits and vegetables by enteric bacteria, including Escherichia coli. E. coli survives in extra-host environments (e.g., soil) and could disperse across landscapes by wildlife. Understanding how terrestrial landscapes impact the distribution of soil E. coli is of importance in assessing the contamination risk of agricultural products. Here, we compared the distribution of E. coli isolated from soils from two watersheds with different landscape patterns in central New York, USA, and examined the influences of two ecological forces - environmental selection and dispersal - on the distribution of E. coli. Results showed that for the watershed with widespread produce fields, sparse forests, and limited interaction between the two land types, E. coli composition was significantly different between produce field and forest; this distribution was shaped by relatively strong environmental selection likely triggered by soil phosphorus and antimony. For the watershed with more forested areas and stronger interaction between produce field and forest, E. coli composition between these two land types was relatively homogeneous; this distribution was a consequence of weak selective pressure potentially from soil moisture and wildlife-driven dispersal by small flocking insectivore/granivores and large nuisance wildlife, which were identified as potential vehicles by competing models. Collectively, our results suggest that terrestrial landscape could drive the biogeographic pattern of enteric bacteria by adjusting the balance between environmental selection and dispersal.IMPORTANCEUnderstanding the ecology of enteric bacteria in extra-host environments is important to allow for development and implementation of strategies to minimize pre-harvest contamination with enteric pathogens. Our findings suggest that watershed landscape is an important factor influencing the ecological drivers and transmission pattern of E. coli. For watersheds with widespread produce fields, E. coli appears to experience local adaptation, likely due to exposure to environmental stresses possibly associated with agricultural activities. In contrast, for watersheds with high forest coverage we found evidence for wildlife-driven dispersal of E. coli, which might facilitate more frequent genetic exchange in this environment. Agricultural areas in such watersheds may have a higher risk of produce contamination due to less environmental constrains and higher potential of transmission of enteric bacteria between locations. The significance of our research lies in exploring ecological principles underlying the biogeographic pattern of enteric bacteria at the regional level, which can inform agricultural, environmental and public health policies that aim to reduce the risk of food contamination by enteric bacteria.

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“…Antimony occurs mainly in inorganic forms as Sb (III) and Sb(V) 17,18 while methyl Sb species (CH3SbX, (CH3)2SbX, (CH3)3Sb) have been reported in seawater, 19,20 freshwater, 3 geothermal waters, 21 sediments and sediment pore waters, 22 bacterial cultures, 23,24 landfill gases 25 and plants. 3,26,27 We have been analysing Sb in environmental matrices for over 20 years and in this paper, we discuss our experiences in measuring the content and speciation of Sb in bacterial cultures, waters, sediments, plants and animal tissues.…”

Section: Introductionmentioning

confidence: 99%

Antimony measurements in environmental matrices: seven considerations

Maher

Krikowa

Foster

et al. 2018

J. Anal. At. Spectrom.

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Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Cited by 152 publications

References 137 publications

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4

Adjacent terrestrial landscapes impact the biogeographical pattern of soil Escherichia coli in produce fields by modifying the importance of environmental selection and dispersal

Antimony measurements in environmental matrices: seven considerations

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