Temperatures in the devitrification process is essential for preserved morphological membrane integrity and sperm function in human spermatozoon

Sánchez, Raúl; Fontecilla, J.; Isachenko, Evgenia; Isachenko, V.; Cabrillana, María E.

doi:10.1016/j.fertnstert.2013.07.1433

Cited by 5 publications

(1 citation statement)

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“…Besides the high-speed freezing (2, 000°C/mL), the warming velocity should also be high so that the water inside spermatozoa passes from glassy state to liquid without ice crystal formation. Using human spermatozoa samples, Sanchez et al [52] found the temperatures in the devitrification process were essential for preserved morphological membrane integrity and sperm function [52]. Furthermore, Mansilla et al [53] tried to determine the optimal warming temperature after human spermatozoa vitrification and found the progressive motility in sperm samples warmed at 42°C (65%) was higher than those at 38°C (26%) and 40°C (57%) and plasma membrane function was also better preserved at 42°C [53].…”

Section: Vitrified Specimen Warmingmentioning

confidence: 99%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

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Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.

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