Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures

Consuegra, C.; Crespo, F.; Dorado, J.; Díaz-Jiménez, María; Pereira, B.; Sánchez‐Calabuig, María Jesús; Beltrán-Breña, P.; Pérez‐Cerezales, Serafín; Rizos, Dimitrios; Hidalgo, M.

doi:10.1016/j.anireprosci.2020.106627

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…In addition, the effect of sucrose, trehalose, and raffinose was evaluated, and a better cryoresponse of equine spermatozoa with 100 mM trehalose was determined. In fact, the in vitro fertilizing ability of the vitrified-warmed equine spermatozoa reached the same fertility percentages as those obtained after conventional freezing [72][73][74].…”

Section: Mechanism Of Flow and Efflux Of Water-glycerol In The Sperma...supporting

confidence: 55%

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

A. Galarza,

Duma,

X. Samaniego

et al. 2023

Veterinary Medicine and Science

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Current sperm cryopreservation protocols for domestic and wild mammals aim to minimize the cryogenic damage caused by cell dehydration, ice formation, and osmotic stress. The optimization of sperm cryopreservation include the use of different synthetic and nonsynthetic-based extenders supplemented with additives (e.g., egg yolk, coconut water, etc.) and antioxidants (e.g., melatonin, L-carnitine, caffeine, resveratrol, etc.) that protect the plasmalemma, acrosome, and mitochondria against the detrimental effects caused by the cryopreservation process. Furthermore, the use of penetrating (e.g., glycerol, ethylene glycol, dimethylformamide, etc.) and nonpenetrating (e.g., sucrose and trehalose) cryoprotectant agents (CPAs) or their combination should be investigated to protect sperm during the freezing process in slow and ultra-rapid freezing procedures. Finally, new cryopreservation protocols should focus on freezing curves and initial cooling rates that allow optimal dehydration during freezing and adequate hydration during thawing. The suitable interaction of all these factors will allow a sperm subpopulation to survive cryopreservation with integrity and fertilizing capacity, contributing to the improvement of the efficiency of genetic resource management and the development of germplasm banks that support the preservation of genetic diversity in domestic and wild animals.

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Section: Mechanism Of Flow and Efflux Of Water-glycerol In The Sperma...supporting

confidence: 55%

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

A. Galarza,

Duma,

X. Samaniego

et al. 2023

Veterinary Medicine and Science

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“…However, it appears that this capacity is not restricted solely to this order. Both, our group and other researchers have 3 found that horse (order Perissodactyla) and guinea pig spermatozoa (order Rodentia) are also capable of fertilizing zona-intact bovine oocytes (20)(21)(22).…”

Section: Introductionmentioning

confidence: 64%

Oviductin sets the species-specificity of the mammalian zona pellucida

de la Fuente,

Maroto,

Cajas

et al. 2024

Preprint

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The zona pellucida (ZP) is vital for species-specific fertilization as this barrier mediates sperm-oocyte binding. Here, we determined whether sperm from distant mammalian orders (Carnivora, Primates, and Rodentia) could penetrate bovine oocytes by examining the role of bovine oviductal fluid and species-specific oviductal glycoprotein (OVGP1 or oviductin) from bovine, murine, or human sources in modulating the species specificity of bovine and murine oocytes. Sperm from all the species were found to penetrate intact bovine ovarian oocytes to form hybrid embryos. However, contact with oviductal fluid or bovine, murine, or human OVGP1, conferred the ZP species specificity, allowing only the penetration of the corresponding sperm regardless of the ZP’s origin. Glycolytic and microstructural analyses revealed that OVGP1 covers the pores present in the ZP and that OVGP1 glycosylation determines sperm specificity. This suggests specific fertilization capacity is acquired in the oviduct through the ZP’s incorporation of specific oviductin.TeaserThe oocyte zona pellucida needs to interact with an oviduct protein called OVGP1 to ensure that only sperm from the same species can fertilize the egg.Abstract FigureModel

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