First pregnancies in jennies with vitrified donkey semen using a new warming method

Díaz-Jiménez, María; Rota, Alessandra; Dorado, J.; Consuegra, C.; Pereira, B.; Camillo, Francesco; Panzani, Duccio; Fanelli, Diana; Tesi, Matteo; Monaco, Davide; Hidalgo, M.

doi:10.1016/j.animal.2020.100097

Cited by 8 publications

(3 citation statements)

References 65 publications

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“…In the research of alternative procedures, Diaz‐Jimenez et al. (2021) described a new direct method of warming. First, the covering straw was opened with forceps and each 0.25‐ml straw with the vitrified sperm was pulled out.…”

Section: Fertilizing Ability Of Vitrified Donkey Spermmentioning

confidence: 99%

Recent advances in donkey sperm vitrification

Hidalgo

2021

Reprod Domestic Animals

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Artificial insemination (AI) with cryopreserved semen is an important tool to preserve endangered species, including European donkey breeds. Sperm vitrification is an alternative method to conventional freezing using high cooling rates and nonpermeable cryoprotectant agents (CPAs). In donkeys, sperm vitrification was firstly developed in spheres by directly dropping the sperm (30 µl) into the liquid nitrogen.

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Section: Fertilizing Ability Of Vitrified Donkey Spermmentioning

confidence: 99%

Recent advances in donkey sperm vitrification

Hidalgo

2021

Reprod Domestic Animals

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“…But then, the low reproductive efficiency of jennies has become an important challenge to overcome (Li et al, 2021). Unfortunately, pregnancy rates in jennies after artificial insemination with frozen-thawed donkey semen are poor (Diaz-Jimenez et al, 2021). This condition could be attributed to the impact of permeable cryoprotectants (Vidament et al, 2009), which could induce high post-breeding uterus inflammation and consequently, affect fertility (Diaz-Jimenez et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, pregnancy rates in jennies after artificial insemination with frozen–thawed donkey semen are poor (Diaz‐Jimenez et al, 2021). This condition could be attributed to the impact of permeable cryoprotectants (Vidament et al, 2009), which could induce high post‐breeding uterus inflammation and consequently, affect fertility (Diaz‐Jimenez et al, 2021). The inflammation caused by spermatozoa is characterized by a rapid influx of polymorphonuclear neutrophils (PMNs) into the uterine lumen after artificial insemination (Troedsson et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma

Páez

Suarez

Betancur

2023

Reprod Domestic Animals

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Cryopreservation of semen is an important technique to preserve genetic material. Yet, pregnancy rates in jennies after artificial insemination with frozen–thawed donkey semen are poor. This condition has been attributed to the impact of permeable cryoprotectants, that could cause high post‐breeding endometritis. Removal of seminal plasma (SP) prior to semen freezing process is another contributing factor. SP is involved in a multitude of sperm functions and events preceding fertilization and has a mediating effect of sperm capacitation and postcoital uterine inflammatory response. The aim of this study was to evaluate different alternatives in donkey semen cryopreservation with permeable, non‐permeable cryoprotectants, BSA and SP. Thirty ejaculates from 10 donkeys were cryopreserved with different combinations of dimethylformamide (DMF, 5%), sucrose (SUC, 200 mM) and homologous SP (10%): DMF (T1), DMF/SP (T2), SUC/BSA (T3), SUC/BSA/SP (T4), DMF/SUC/BSA (T5), DMF/SUC/BSA/SP (T6), DMF/BSA (T7) and DMF/BSA/SP (T8). After thawing, sperm motility and kinetics were assessed by computerized semen analysis. Sperm vitality (SV) was evaluated by fluorescence microscopy, functional membrane integrity (FMI) by the HOST test, abnormal morphology by eosin‐nigrosin staining and sperm membrane stability by flow cytometry. For statistical analysis, sperm quality indexes (SQi) were obtained, general linear models were carried out and mean comparisons were made by the Tukey test. T1, T2, T5, T6, and T7 had higher and equivalent results for motility, most kinetic parameters and function membrane integrity. Cryopreservation of donkey semen without permeable cryoprotectant (T3 and T4) showed a reduction in motility, kinetics, SV, FMI and SQi. T5 showed a reduction in progressive motility, sperm velocities, IMF and SQi compared to other DMF treatments. T6 and T8 achieved higher SQi values compared to T1, but they were not different compared to T2 and T7. T1 had a smaller sperm population with low‐M540 compared to T3. It is concluded that the use of permeable cryoprotectant is essential to achieve higher post‐thaw quality of donkey semen. In addition, the combined use of BSA, SUC and/or PS may provide additional sperm protection compared to the individual use of DMF.

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