Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells

Li, Shaoshan; Paulsson, Markus; Björn, Lars Olof

doi:10.1016/s1011-1344(01)00277-9

Cited by 64 publications

(49 citation statements)

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“…This is within the range expected for an enzymemediated response, and is consistent with previous research on the effects of temperature on photolyase activity and photoreactivation response. The photoreactivation rate increases with increasing temperature (warming) in a wide range of organisms including ciliates (Sanders et al, 2005), freshwater crustaceans Daphnia pulicaria (Macfadyen et al, 2004), marine macroalgae Palmaria palmata (Pakker et al, 2000) and tobacco cells (Li et al, 2002). An increase in photoreactivation is expected based on the understanding of photolyase enzyme structure and kinetics.…”

Section: Discussionmentioning

confidence: 99%

DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species

Lamare

Barker

Lesser

et al. 2006

Journal of Experimental Biology

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Over recent geological time the Antarctic marine system has been a relatively low ultraviolet radiation (UV-R) environment because of its polar location, high atmospheric ozone concentrations, and extensive seasonal covering by relatively opaque, and highly reflective, sea ice. Recent increases in ultraviolet-B radiation (280-315·nm) due to stratospheric ozone depletion have highlighted the susceptibility of marine ecosystems to this important abiotic factor (Smith et al., 1992;Karentz, 1994;Smith and Cullen, 1995). The occurrence of the spring ozone hole coincides with the development of embryos of many Antarctic marine invertebrates that may be susceptible to the effects of UV-R because of their small size, lack of a protective tegument, and high rate of cell division (Johnsen and Widder, 2001). Antarctic embryos may be especially vulnerable because they have unique physiological adaptations to survive the cold, food-poor Antarctic waters (cf. Marsh et al., 2001).Our recent observations confirm that Antarctic marine larvae are very sensitive to UV-R (Lesser et al., 2004). Echinoid embryos exposed to ambient UV-R under annual Antarctic sea ice (a low UV-R environment with irradiances р1% of surface irradiances), exhibited significantly higher rates of mortality, abnormal development (Ϸ30-50%), and DNA damage than embryos exposed concurrently but under a UV-R filter. Biological weighting functions for DNA damage and survival To determine if an Antarctic species repairs DNA at rates equivalent to warmer water equivalents, we examined repair of UV-damaged DNA in echinoid embryos and larvae. DNA repair by photoreactivation was compared in three species Sterechinus neumayeri (Antarctica), Evechinus chloroticus (New Zealand) and Diadema setosum (Tropical Australia) spanning a latitudinal gradient from polar (77.86°S) to tropical (19.25°S) environments. We compared rates of photoreactivation as a function of ambient and experimental temperature in all three species, and rates of photoreactivation as a function of embryonic developmental stage in Sterechinus. DNA damage was quantified from cyclobutane pyrimidine dimer (CPD) concentrations and rates of abnormal embryonic development. This study established that in the three species and in three developmental stages of Sterechinus, photoreactivation was the primary means of removing CPDs, was effective in repairing all CPDs in less than 24·h, and promoted significantly higher rates of normal development in UV-exposed embryos. CPD photorepair rate constant (k) in echinoid embryos ranged from 0.33 to 1.25·h -1 , equating to a time to 50% repair of between 0.6 and 2.1·h and time to 90%repair between 3.6 and 13.6·h. We observed that experimental temperature influenced photoreactivation rate. In Diadema plutei, the photoreactivation rate constant increased from k=0.58·h -1 to 1.25·h -1 , with a Q 10 =2.15 between 22°C and 32°C. When compared among the three species across experimental temperatures (-1.9 to 32°C), photoreactivation rates vary with a Q 10 =1.39. Photoreactivati...

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Section: Discussionmentioning

confidence: 99%

DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species

Lamare

Barker

Lesser

et al. 2006

Journal of Experimental Biology

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“…Only the activity of the antioxidant enzyme catalase has been shown not to be thermally sensitive in response to UVR exposure in L. peronii tadpoles [72]. However, the DNA repair mechanisms of enzymatic photoreactivation (EPR) and nucleotide excision repair (NER) have been shown to increase with increasing temperature in ciliates [115], freshwater crustaceans [116], marine macroalgae [117], tobacco cells [118] and echinoid embryos [119], however, Connelly et al [120] found the opposite to be true in Daphnia. The photoprotective mechanism of ROS reduction by enzymatic antioxidant activity has also been found to be thermally sensitive in summer-caught mosquito fish Gambusia holbrooki exposed to UVR [39].…”

Section: Uvr and Abiotic Factors Temperaturementioning

confidence: 99%

Drivers of amphibian declines: effects of ultraviolet radiation and interactions with other environmental factors

Alton

Franklin

2017

Clim Chang Responses

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As a consequence of anthropogenic environmental change, the world is facing a possible sixth mass extinction event. The severity of this biodiversity crisis is exemplified by the rapid collapse of hundreds of amphibian populations around the world. Amphibian declines are associated with a range of factors including habitat loss/ modification, human utilisation, exotic/invasive species, environmental acidification and contamination, infectious disease, climate change, and increased ultraviolet-B radiation (UVBR) due to stratospheric ozone depletion. However, it is recognised that these factors rarely act in isolation and that amphibian declines are likely to be the result of complex interactions between multiple anthropogenic and natural factors. Here we present a synthesis of the effects of ultraviolet radiation (UVR) in isolation and in combination with a range of naturally occurring abiotic (temperature, aquatic pH, and aquatic hypoxia) and biotic (infectious disease, conspecific density, and predation) factors on amphibians. We highlight that examining the effects of UVR in the absence of other ecologically relevant environmental factors can greatly oversimplify and underestimate the effects of UVR on amphibians. We propose that the pathways that give rise to interactive effects between multiple environmental factors are likely to be mediated by the behavioural and physiological responses of amphibians to each of the factors in isolation. A sound understanding of these pathways can therefore be gained from the continued use of multi-factorial experimental studies in both the laboratory and the field. Such an understanding will provide the foundation for a strong theoretical framework that will allow researchers to predict the combinations of abiotic and biotic conditions that are likely to influence the persistence of amphibian populations under future environmental change.

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“…We found that one kind of damage called CPD is repaired almost equally fast at 12°C as at 24°C, but only very slowly at 0°C. [3][4][5] Repair of another kind of damage to DNA, called 6-4 photoproducts, [4][5][6] was considerably slower already at 12°C as compared with at 24°C. An unexpected result is that, in addition, the damage process under UV-B radiation in these cells was more rapid at 24°C as compared with at 0°C.…”

Section: Dna Damage and Repairmentioning

confidence: 99%

Sensing of UV-B radiation by plants

Jiang

Wang²,

Björn

et al. 2012

Plant Signaling & Behavior

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Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells

Cited by 64 publications

References 10 publications

DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species

DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species

Drivers of amphibian declines: effects of ultraviolet radiation and interactions with other environmental factors

Sensing of UV-B radiation by plants

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