Sensing of UV-B radiation by plants

Jiang, Lei; Wang, Yan; Björn, Lars Olof; He, Jun; Li, Shaoshan

doi:10.4161/psb.20815

Cited by 40 publications

(42 citation statements)

References 35 publications

(48 reference statements)

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“…HY5 is the major transcription factor required downstream of UVR8. In addition, the HY5 gene is an excellent and widely used marker for UVR8-mediated gene expression changes (Ulm et al, 2004;Brown et al, 2005Brown et al, , 2009Oravecz et al, 2006;Gruber et al, 2010;Huang et al, 2012;Jiang et al, 2012). Thus, it is thought that HY5 is required for UV-Bresponsive gene activation and that its own transcriptional induction as well as posttranslational stabilization further enforce the UV-B response Tilbrook et al, 2013;Jenkins, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…their UV-B-induced expression Huang et al, 2012;Jiang et al, 2012) and further underlines the regulatory activity of HY5 chromatin association in UV-B signaling.…”

Section: Hy5 Binds To Genes Encoding Proteins Involved In Uv-b Signalmentioning

confidence: 99%

“…FHY3 binds to an FHY3 binding site and HY5 to an ACGT-containing element in the COP1 promoter, both of which seem to be required for the UV-B responsiveness of COP1 . Combinatorial regulation exerted by the interaction of other transcription factors with HY5/HYH is widely present, with negative or positive regulatory effects (Shin et al, 2007;Andronis et al, 2008;Holtan et al, 2011;Huang et al, 2012;Jiang et al, 2012;Singh et al, 2012;Gangappa et al, 2013aGangappa et al, , 2013bJing et al, 2013;Abbas et al, 2014). Moreover, HY5 interacts with the chromatin-remodeling factor PICKLE (PKL), recruiting PKL to promoters of target genes repressing the H3K27me3-repressive histone mark (Jing et al, 2013).…”

Section: Regulation Of the Hy5 Association With Chromatinmentioning

confidence: 99%

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UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

et al. 2014

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In plants subjected to UV-B radiation, responses are activated that minimize damage caused by UV-B. The bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) and promotes UV-B-induced photomorphogenesis and acclimation. Expression of HY5 is induced by UV-B; however, the transcription factor(s) that regulate HY5 transcription in response to UV-B and the impact of UV-B on the association of HY5 with its target promoters are currently unclear. Here, we show that HY5 binding to the promoters of UV-B-responsive genes is enhanced by UV-B in a UVR8-dependent manner in Arabidopsis thaliana. In agreement, overexpression of REPRESSOR OF UV-B PHOTOMORPHOGENESIS2, a negative regulator of UVR8 function, blocks UV-B-responsive HY5 enrichment at target promoters. Moreover, we have identified a T/G-box in the HY5 promoter that is required for its UV-B responsiveness. We show that HY5 and its homolog HYH bind to the T/G HY5 -box cis-acting element and that they act redundantly in the induction of HY5 expression upon UV-B exposure. Therefore, HY5 is enriched at target promoters in response to UV-B in a UVR8 photoreceptor-dependent manner, and HY5 and HYH interact directly with a T/G-box cis-acting element of the HY5 promoter, mediating the transcriptional activation of HY5 in response to UV-B.

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Section: Discussionmentioning

confidence: 99%

“…their UV-B-induced expression Huang et al, 2012;Jiang et al, 2012) and further underlines the regulatory activity of HY5 chromatin association in UV-B signaling.…”

Section: Hy5 Binds To Genes Encoding Proteins Involved In Uv-b Signalmentioning

confidence: 99%

Section: Regulation Of the Hy5 Association With Chromatinmentioning

confidence: 99%

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UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

et al. 2014

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“…COP1 was seen to interact with the UVR8 W285A constitutive monomer regardless of UV-B exposure, both in yeast and co-immunoprecipitations from plant tissues. Conversely, the UVR8-COP1 interaction was abolished with the UVR8 W285F constitutive dimer (Koornneef et al 1980) bZIP transcription factor (Oyama et al 1997) COP1 (Ang et al 1998) BBX24 (Jiang et al 2012) FHY3 ) HYH (Holm et al 2002) Positive regulator (Ulm et al 2004) At3g17609 HYH (HY5-HOMOLOG) (Holm et al 2002) bZIP transcription factor (Holm et al 2002) COP1 (Holm et al 2002) HY5 (Holm et al 2002) Positive , does not seem to result in constitutive UV-B responses in transgenic plants . This raises an interesting question concerning the exact mechanistic role of the UVR8-COP1 interaction in UV-B signaling.…”

Section: Positive Regulatorsmentioning

confidence: 99%

“…A recent addition to molecular UV-B signaling is SALT TOL-ERANCE/BBX24 (STO/BBX24, herein referred to as BBX24; At1g06040), which is proposed to act as a negative regulator of the pathway (Jiang et al 2012). BBX24 was initially characterised as conferring salt tolerance when expressed in yeast (Lippuner et al 1996), as well as in plants (Nagaoka and Takano 2003).…”

Section: The B-box Protein Sto/bbx24mentioning

confidence: 99%

The UVR8 UV-B Photoreceptor: Perception, Signaling and Response

Tilbrook

Arongaus

Binkert

et al. 2013

The Arabidopsis Book

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Ultraviolet-B radiation (UV-B)is an intrinsic part of sunlight that is accompanied by significant biological effects. Plants are able to perceive UV-B using the UV-B photoreceptor UVR8 which is linked to a specific molecular signaling pathway and leads to UV-B acclimation. Herein we review the biological process in plants from initial UV-B perception and signal transduction through to the known UV-B responses that promote survival in sunlight. The UVR8 UV-B photoreceptor exists as a homodimer that instantly monomerises upon UV-B absorption via specific intrinsic tryptophans which act as UV-B chromophores. The UVR8 monomer interacts with COP1, an E3 ubiquitin ligase, initiating a molecular signaling pathway that leads to gene expression changes. This signaling output leads to UVR8-dependent responses including UV-B-induced photomorphogenesis and the accumulation of UV-B-absorbing flavonols. Negative feedback regulation of the pathway is provided by the WD40-repeat proteins RUP1 and RUP2, which facilitate UVR8 redimerization, disrupting the UVR8-COP1 interaction. Despite rapid advancements in the field of recent years, further components of UVR8 UV-B signaling are constantly emerging, and the precise interplay of these and the established players UVR8, COP1, RUP1, RUP2 and HY5 needs to be defined. UVR8 UV-B signaling represents our further understanding of how plants are able to sense their light environment and adjust their growth accordingly.

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