Technological advancements in valorization of second generation (2G) feedstocks for bio-based succinic acid production

Narisetty, Vivek; Okibe, Maureen Chiebonam; Amulya, K; Jokodola, Esther Oreoluwa; Coulon, Frédéric; Tyagi, Vinay Kumar; Lens, P.N.L.; Kumar, Vinod

doi:10.1016/j.biortech.2022.127513

Cited by 32 publications

(24 citation statements)

References 97 publications

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“…Currently, there are few reports in the literature that utilise BW for fermentative SA production, which need to be tested in pilot scales to understand its technical feasibility and commercial viability. Biochemically, SA is an intermediate of TCA (tricarboxylic acid) cycle and microorganisms produce SA via both oxidative and reductive pathways (Narisetty et al, 2022c;Oreoluwa Jokodola et al, 2022). Leung et al (2012) extracted sugars and amino acids from BW using glucoamylase and protease from fungal strains, Aspergillus awamori and Aspergillus oryzae, respectively.…”

Section: Succinic Acid (Sa)mentioning

confidence: 99%

Bread waste – A potential feedstock for sustainable circular biorefineries

Kumar

Brancoli

Narisetty

et al. 2023

Bioresource Technology

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Section: Succinic Acid (Sa)mentioning

confidence: 99%

Bread waste – A potential feedstock for sustainable circular biorefineries

Kumar

Brancoli

Narisetty

et al. 2023

Bioresource Technology

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“…Production of chemicals and fuels from renewable resources is a promising route for sustainable development in the future. The chemical synthesis of chemicals has a risk of environmental pollution, and the sustainable production of a microbial fermentation method is increasingly favored [1][2][3]. Succinic acid (SA), one intermediate of the tricarboxylic acid cycle (TCA), is a widely high-value chemical and is applied in numerous fields, including food, chemicals, agriculture, and pharmaceuticals [1,4].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, genes associated with sugar transporters and SA transporters were mined and identified, which will useful in improving the fermentative production of SA by A. succinogenes. Many microorganisms, such as Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens, Escherichia coli, Corynebacterium glutamicum, Yarrowia lipolytica, and Saccharomyces cerevisiae, have been developed for SA production [2,[12][13][14][15]. A. succinogenes is facultative anaerobic and gram-negative, and is isolated from the bovine rumen [16].…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Biosynthesis of Succinic Acid by Actinobacillus succinogenes with the Help of Its Engineered Strains

Chen,

Zheng

2023

Fermentation

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Succinic acid (SA), a C4 tricarboxylic acid cycle intermediate, is used as raw material for bulk chemicals and specialty chemicals, such as tetrahydrofuran and 1,4-butanediol, as well as also being used to synthesize the biodegradable biopolymers PBS (polymer poly (butylene succinate)). Actinobacillus succinogenes, which is facultative anaerobic and gram-negative, is one of the most promising natural SA-producing organisms, but genetic engineering of A. succinogenes is rare so far. In this study, a series of engineered strains was constructed using the pLGZ922 expression vector and a cytosine base editor (CBE) based on CRIPSR/Cas9; we found that phosphoenolpyruvate carboxylase (PEPC) was more important for the CO2 fixation pathway than pyruvate carboxylase (PYC) in A. succinogenes, and the annotated oxaloacetic acid decarboxylase (Asuc_0301 and Asuc_0302) had little correlation with the SA synthesis pathway. The by-product pathway was closely related to cell growth, and overexpression of FDH was beneficial to growth, while the knockout of the ackA gene reduced the growth. For the first time, the hypothetic sugars and SA transporters were mined and identified in A. succinogenes, of which Asuc_0914 was responsible for glucose uptake, and Asuc_0715 and Asuc_0716 constituted SA exporters. This deepens the understanding of SA biosynthesis in A. succinogenes and is also valuable for SA production by fermentation.

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“… 8 , 9 Several bacterial and yeast strains have been employed for the bioproduction of SA from various carbon sources. 10 − 12 The bacterial strains are very sensitive to pH fluctuations and require pH control between 6.0 and 8.0, yielding SA in salt rather than the acid form. The existence of SA in the salt form complicates downstream processing and makes the process expensive.…”

Section: Introductionmentioning

confidence: 99%

“…The traditional route of SA production involves either carbonylation of ethylene glycol, oxidation of 1,4-butanediol, or hydrogenation of maleic acid. However, due to the unsustainability and risks of chemical routes on the environment, biological processes involving renewable feedstocks have gained significant interest. , Several bacterial and yeast strains have been employed for the bioproduction of SA from various carbon sources. − The bacterial strains are very sensitive to pH fluctuations and require pH control between 6.0 and 8.0, yielding SA in salt rather than the acid form. The existence of SA in the salt form complicates downstream processing and makes the process expensive .…”

Section: Introductionmentioning

confidence: 99%

Development of Hypertolerant Strain of Yarrowia lipolytica Accumulating Succinic Acid Using High Levels of Acetate

Narisetty

Prabhu

Bommareddy

et al. 2022

ACS Sustainable Chem. Eng.

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Acetate is emerging as a promising feedstock for biorefineries as it can serve as an alternate carbon source for microbial cell factories. In this study, we expressed acetyl-CoA synthase in Yarrowia lipolytica PSA02004PP, and the recombinant strain grew on acetate as the sole carbon source and accumulated succinic acid or succinate (SA). Unlike traditional feedstocks, acetate is a toxic substrate for microorganisms; therefore, the recombinant strain was further subjected to adaptive laboratory evolution to alleviate toxicity and improve tolerance against acetate. At high acetate concentrations, the adapted strain Y. lipolytica ACS 5.0 grew rapidly and accumulated lipids and SA. Bioreactor cultivation of ACS 5.0 with 22.5 g/L acetate in a batch mode resulted in a maximum cell OD 600 of 9.2, with lipid and SA accumulation being 0.84 and 5.1 g/L, respectively. However, its fed-batch cultivation yielded a cell OD 600 of 23.5, SA titer of 6.5 g/L, and lipid production of 1.5 g/L with an acetate uptake rate of 0.2 g/L h, about 2.86 times higher than the parent strain. Cofermentation of acetate and glucose significantly enhanced the SA titer and lipid accumulation to 12.2 and 1.8 g/L, respectively, with marginal increment in cell growth (OD 600 : 26.7). Furthermore, metabolic flux analysis has drawn insights into utilizing acetate for the production of metabolites that are downstream to acetyl-CoA. To the best of our knowledge, this is the first report on SA production from acetate by Y. lipolytica and demonstrates a path for direct valorization of sugar-rich biomass hydrolysates with elevated acetate levels to SA.

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Technological advancements in valorization of second generation (2G) feedstocks for bio-based succinic acid production

Cited by 32 publications

References 97 publications

Bread waste – A potential feedstock for sustainable circular biorefineries

Bread waste – A potential feedstock for sustainable circular biorefineries

New Insights into the Biosynthesis of Succinic Acid by Actinobacillus succinogenes with the Help of Its Engineered Strains

Development of Hypertolerant Strain of Yarrowia lipolytica Accumulating Succinic Acid Using High Levels of Acetate

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