Techniques for the <i>in vivo</i> assessment of cardio‐renal function in zebrafish (<i>Danio rerio</i>) larvae

Rider, Sebastien A.; Tucker, Carl; Del-Pozo, Jorge; Rose, Kirsten N.; MacRae, Calum A.; Bailey, Matthew A.; Mullins, John J.

doi:10.1113/jphysiol.2011.224352

Cited by 36 publications

(45 citation statements)

References 11 publications

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“…Further, whole-mount immunofluorescence demonstrated that gentamicin injected fish had a disruption of cell polarity, evidenced by diffusion of anti-Na + /K + ATPase α-subunit (α6F) in the cytoplasm, which is typically concentrated in the basolateral membrane of proximal tubular cells. Taken together, these data demonstrated the many similarities between gentamicin-induced pronephric injury in zebrafish and mammalian AKI, consistent with prior observations [32–34]. …”

Section: Insights Into Aki From Chemical Geneticssupporting

confidence: 91%

Congenital and Acute Kidney Disease: Translational Research Insights from Zebrafish Chemical Genetics

Poureetezadi

Wingert

2013

General Med

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Today, acute kidney injury (AKI) and congenital anomalies of the kidney and urinary tract (CAKUT) represent major issues in healthcare. Both AKI and CAKUT can lead to end stage renal disease (ESRD) that requires life-long medical care with renal replacement therapy. Renal replacement by dialysis is intensive, and kidney transplantation is restricted by organ availability. These limitations, along with the growing epidemic of patients affected by kidney disease, highlight the significant need to identify alternative ways to treat renal injury and birth defects. Drug discovery is one promising avenue of current research. Here, we discuss zebrafish chemical genetics and its latent potency as a method to rapidly identify small molecule therapeutics to accelerate recovery after AKI. Specifically, we review two groundbreaking studies that have recently provided a template to screen for compounds that expand the renal progenitor field in development that were capable of treating AKI in both the zebrafish and the mouse. These new findings demonstrate that drug discovery using zebrafish can be used for relevant translational research to identify clinical interventions for renal conditions in humans.

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Section: Insights Into Aki From Chemical Geneticssupporting

confidence: 91%

Congenital and Acute Kidney Disease: Translational Research Insights from Zebrafish Chemical Genetics

Poureetezadi

Wingert

2013

General Med

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“…Salinity solutions were prepared as previously described (24,60). Captopril (C4042, Sigma-Aldrich) was administered at 0.1 mM.…”

Section: Methodsmentioning

confidence: 99%

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Rider

Mullins

Verdon

et al. 2015

American Journal of Physiology-Renal Physiology

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Rider SA, Mullins LJ, Verdon RF, MacRae CA, Mullins JJ. Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium. Am J Physiol Renal Physiol 309: F531-F539, 2015. First published July 22, 2015 doi:10.1152/ajprenal.00247.2015.-Although renin is a critical regulatory enzyme of the cardiovascular system, its roles in organogenesis and the establishment of cardiovascular homeostasis remain unclear. Mammalian renin-expressing cells are widespread in embryonic kidneys but are highly restricted, specialized endocrine cells in adults. With a functional pronephros, embryonic zebrafish are ideal for delineating the developmental functions of renin-expressing cells and the mechanisms governing renin transcription. Larval zebrafish renin expression originates in the mural cells of the juxtaglomerular anterior mesenteric artery and subsequently at extrarenal sites. The role of renin was determined by assessing responses to renin-angiotensin system blockade, salinity variation, and renal perfusion ablation. Renin expression did not respond to renal flow ablation but was modulated by inhibition of angiotensin-converting enzyme and altered salinity. Our data in larval fish are consistent with conservation of renin's physiological functions. Using transgenic renin reporter fish, with mindbomb and cloche mutants, we show that Notch signaling and the endothelium are essential for developmental renin expression. After inhibition of angiogenesis, renin-expressing cells precede angiogenic sprouts. Arising from separate lineages, but relying on mutual interplay with endothelial cells, renin-expressing cells are among the earliest mural cells observed in larval fish, performing both endocrine and paracrine functions. renin; zebrafish; notch; endothelium; angiogenesis RENIN is the rate-limiting enzyme of the renin-angiotensin system (RAS). Mammalian ANG II, the effector of the RAS, is principally involved in blood pressure homeostasis by regulation of vasomotor tone and Na ϩ retention. ANG II also regulates cell proliferation and angiogenesis (12,22). Pathological activation of the RAS is associated with cardiovascular diseases, including hypertension and heart failure, and is consequently a major target of therapeutic agents (11).Renin and its cognate cells are required for normal renal development (2), including angiogenesis of the renal vascular tree (59). In mice, ablation of the renin gene or renin-expressing cells leads to renal developmental abnormalities (55,71,79). The vasculature of mammalian embryonic kidneys have a transient but extensive coverage of mural renin-expressing cells (17,31,32,47,63); however, their function is unclear. Adult renin-expressing cells are restricted to the juxtaglomerular apparatus and play a vital role in ion homeostasis by secreting activated renin enzyme via cytoplasmic granules (17,31,36,63). Upon physiological challenge by RAS inhibition or low Na ϩ , prearteriolar vascular smooth muscle cells (VSMC) reestablish their embryonic re...

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“…It is no surprise that since zebrafish are an excellent animal model they have also been utilized in studying the kidney [23][24][25] . To date, zebrafish have been used to advance our knowledge of renal development as well as aspects of several kidney disease phenotypes [26][27][28][29][30][31][32] . In particular, mutagenesis screens in zebrafish have been used to identify genes that cause kidney malformations, which led to the discovery of ciliogenesis genes that cause renal cyst formation when disrupted [33][34][35][36][37][38] .…”

Section: The Zebrafish: a Model For Kidney Researchmentioning

confidence: 99%

Insights into kidney stem cell development and regeneration using zebrafish

Drummond

Wingert

2016

WJSC

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Kidney disease is an escalating global health problem, for which the formulation of therapeutic approaches using stem cells has received increasing research attention. The complexity of kidney anatomy and function, which includes the diversity of renal cell types, poses formidable challenges in the identification of methods to generate replacement structures. Recent work using the zebrafish has revealed their high capacity to regenerate the integral working units of the kidney, known as nephrons, following acute injury. Here, we discuss these findings and explore the ways that zebrafish can be further utilized to gain a deeper molecular appreciation of renal stem cell biology, which may uncover important clues for regenerative medicine.

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Techniques for the in vivo assessment of cardio‐renal function in zebrafish (Danio rerio) larvae

Cited by 36 publications

References 11 publications

Congenital and Acute Kidney Disease: Translational Research Insights from Zebrafish Chemical Genetics

Congenital and Acute Kidney Disease: Translational Research Insights from Zebrafish Chemical Genetics

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Insights into kidney stem cell development and regeneration using zebrafish

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