Abstract-Co-digestion of cassava and winery waste was investigated for the production of biogas. Cassava biomass is a good substrate due to its high carbohydrate yield per hectare (4.742kg/carb) than most plants. Winery waste is a growing problem in South Africa due to high amounts currently being dumped at landfills. Due to the chemical properties of the two substrates it is envisaged that their co-digestion will produce more biogas than use of a single substrate. Biomethane potential (BMP) tests were carried out in a batch, mesophilic (37˚C±0.5) reactor using cassava and winery waste singly and in combination at a ratio of 1:1 and run for 30 days. The results showed that cumulative methane yield for cassava, winery waste and in combination were 42, 21 and 38 mLCH 4 respectively. It was concluded that biogas production from anaerobic digestion was dependent on many factors such as pH, substrate properties and the ratio of different feedstocks used during co-digestion.