Taxonomical Re-examination of Two Pathogenic Vibrio Species Isolated from Milkfish and Swimming Crab.

Abstract

A taxonomical re-examination of two pathogenic bacteria belonging to the genus Vibrio, one from an eye disease of milkfish (Chanos chanos) in the Philippines and the other from vibriosis of larval swimming crab (Portunus trituberculatus) in Japan, was made by examining their phenotypic features and measuring DNA-DNA relatedness.As a result, the bacterium from milkfish was identified as V. harveyi based on the high DNA homology value of above 84% between the two milkfish isolates and the type strain of V. harveyi and coincidence in phenotypic characteristics.Strain 85Z-1, the representative strain of the causative bacterium (Vibrio sp. Zoea) of vibriosis in swimming crab, showed higher DNA relatedness than 75% to other 3 strains of Vibrio sp. Zoea, about 50% relatedness to strains of V. harveyi, and lower relatedness than 46% to the other species examined.These results indicate that Vibrio sp. Zoea is a new genomic species most closely to V. harveyi.However, it is difficult to distinguish phenotypically Vibrio sp. Zoea from V. harveyi isolated from milkfish, thus species name for Vibrio sp. Zoea could not be proposed.

Taxonomic evidence that Vibrio carchariae Grimes et al. 1985 is a junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981

“…In the present study, AFLP fingerprinting was used successfully to differentiate between strains in the collection. (Ishimaru & Muroga, 1997). In the present study, the two Vibrio sp.…”

Türkiye’de Kültürü Yapılan Sinarit Balıklarında (Dentex dentex) İlk Vibrio harveyi Enfeksiyonu Bildirimi

“…harveyi is a well-known pathogen of both invertebrates and vertebrates and infections in fish by this bacterium have been reported from both wild and cultured species (Austin and Zhang, 2006). Eye lesions have been reported as a common clinical finding in several disease outbreaks, affecting common dentex (D. dentex) (Company et al, 1999), gilthead sea bream (Sparus aurata) (Haldar et al, 2010), milkfish (Chanos chanos) (Ishimaru and Muroga, 1997), common snook (Centropomus undecimalis) (Kraxberger-Beatty et al, 1990) and short sunfish (Mola mola) (Hispano et al, 1997). In accordance with these reports, all individuals had corneal opacities in our examination.…”

Pathogenicity of Vibrio harveyi to salmonids

“…Sunaryanto & Mariam 1986; Karunasagar, Pai, Malathi & Karunasagar 1994; Pizzutto & Hirst 1995; Liu, Lee, Yii, Kou & Chen 1996a; Robertson, Calderon, Carrera, Stark, Zherdmant & Austin 1998; Vandenberghe, Li, Verdonck, Li, Sorgeloos, Xu & Swings 1998) and fish (e.g. Kraxberger‐Beatty, McGarey, Grier & Lim 1990; Ishimaru & Muroga 1997; Alvarez, Austin, Alvarez & Reyes 1998). The organism is synonymous with V. carchariae ( Pedersen, Verdonck, Austin, Austin, Blanch, Grimont, Jofre, Koblavi, Larsen, Tiainen, Vigneulle & Swings 1998), which is also a fish pathogen (e.g.…”