Pathogenicity of Vibrio harveyi to salmonids

Abstract

Out of 19 Vibrio harveyi isolates obtained from a diversity of hosts and geographical locations, 14 were pathogenic to rainbow trout, Oncorhynchus mykiss (Walbaum), and Atlantic salmon, Salmo salar L., with mortalities of up to 100% following intraperitoneal injections of 106 cells fish−1. The extracellular products (ECPs) of only five pathogenic isolates were harmful to fish. Both pathogenic and non-pathogenic cultures produced ECPs containing caseinase, gelatinase, phospholipase, lipase and haemolysins. Vibrio harveyi VIB 645, which was the most pathogenic isolate, produced ECPs with a maximal effect on salmonids from preparations obtained by using cellophane overlays on tryptone soya agar supplemented with 1% (w/v) sodium chloride with incubation at 28 °C for 24 h. This preparation contained the highest titre of haemolytic activity to Atlantic salmon (1:256) and rainbow trout (1:32) erythrocy

PERBANDINGAN PATOGENESITAS, Edwardsiella tarda PADA IKAN MAS KOKI (Charassius auratus) DAN IKAN CELEBES RAINBOW (Telmatherina celebensis)

“…Patogenitas bakteri dapat ditunjukkan dari aktivitas hemolitik pada darah dan organ internal ikan (Zhang & Austin, 2000). Patogenitas E. tarda dipengaruhi oleh banyak faktor, dan kemungkinan pula penyebaran penyakit ini sudah terjadi, seperti antiphagocyte killing (Ainsworth & Chen, 1983), hemolysin (Hirono et al, 1997), serum resistance, dan kemampuan sel epthelial (Janda et al, 1991;Ling et al, 2000).…”

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

“…Recently, Vibrio harveyi has appeared to be a primary pathogen infecting hatchery-reared fish and shellfish species over a wide geographical area (Pass et al, 1987;Pizzutto and Hirst, 1995;Vandenberghe et al, 1998;Zhang and Austin, 2000). Mortalities in hatchery-reared penaeid shrimps caused by Vibrio harveyi infection has resulted in severe economic losses in producing countries in Asia (Karunasagar et al, 1994;Lavilla-Pitogo et al, 1990;Liu et al, 1996).…”

“…Studies conducted by Zhang and Austin (2000) suggested that hemolysins are one of the pathogenic determinants of several V. harveyi strains isolated from a variety of hosts and geographic origins. Subsequently, they isolated the duplicated hemolysin genes (vhhA and vhhB) of a strain of V. harveyi pathogenic to salmons (Zhang et al, 2001).…”

Effect of combined function of temperature and water activity on the growth of Vibrio harveyi

“…Although V. harveyi is non pathogenic to human, it is one of causative agents of the systemic fish disease, vibriosis, and sea food spoilage which occurs in many commercially important fish (20), including sharks, seahorse, lobster, shellfish or shrimps (13). Pseudosciaena crocea (big yellow croaker) is an important commercial marine fish in China, and has been widely cultured in hatcheries recent years.…”