Taxonomic study on Vicia unijuga using principal components analysis

Seok, Dong-Im; Choe, Byeong-Hui

doi:10.11110/kjpt.1997.27.3.359

Cited by 4 publications

(8 citation statements)

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“…V. unijuga , V. unijuga var. kaussanensis , V. linearifolia , and V. unijuga f. angustifolia are known to be related taxa, and all possess two leaflets ( Lee, 1972 ; Seok and Choi, 1997 ). V. unijuga var.…”

Section: Discussionmentioning

confidence: 99%

Identification of Vicia Species Native to South Korea Using Molecular and Morphological Characteristics

et al. 2021

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Recently, within the Fabaceae family, the Vicia genus has been recognized for its vital role in sustainable agriculture. Vicia species are economically important grain and forage crops. However, the presence of complex morphological characteristics makes identification and recognition of native species difficult. In this study, the possibility of using DNA barcoding regions (ITS2, matK, and rbcL) to distinguish among 19 Vicia taxa (59 accessions) found in South Korea was evaluated. The sequence alignment analysis revealed considerable nucleotide diversity (π) between the loci, in which ITS2 showed the highest mean interspecific distance, whereas there was no intraspecific variability among the barcode regions in 12 of the 19 taxa. Phylogenetic analysis of combined barcoding regions revealed well-resolved phylogeny with the highest species level discrimination. Combinations of barcode loci were also used in classification at the subgenera and section levels. The results revealed that the combined barcoding regions can be used effectively to differentiate the following species: Vicia angustifolia var. segetilis, Vicia bungei, Vicia villosa, Vicia cracca, Vicia dasycarpa, Vicia hirsuta, Vicia tetrasperma, Vicia amurensis, Vicia hirticalycina, and Vicia chosenensis. However, it is difficult to differentiate the species of Vicia unijuga, Vicia unijuga var. kaussanensis, Vicia linearifolia, Vicia unijuga f. angustifolia, Vicia nipponica, Vicia amoena, Vicia venosa var. cuspidata, Vicia pseudo-orobus, and Vicia japonica with the tested barcode regions. These species come under sect. Vicilla and are found to be closely related or species that have recently undergone speciation; thus, it has limitation to distinguish with recommended barcodes. Hence, to differentiate the unclassified species, 39 morphological characteristics were investigated, in which 16 useful characteristics were selected for efficient classification. Finally, the 16 selected morphological useful traits efficiently differentiated all the Vicia species. In conclusion, a combination of barcoding loci together with morphological characteristics of this study efficiently discriminated all the Korean Vicia species.

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Section: Discussionmentioning

confidence: 99%

Identification of Vicia Species Native to South Korea Using Molecular and Morphological Characteristics

et al. 2021

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“…Among the infraspecific taxa of V. unijuga, var. ouensanensis, which was recorded in Korea basically by the characteristics of leaflet shapes (Ldveilld, 1913), could not be distinguished from wm unijuga because of the variation in its diagnostic character of the leaflet shapes (l,ce, 1969;Seok and Choi, 1997 (Lee, 1982). The leaflet shape of the species, however, showed a clinal variation with that of V. unijuga vat.…”

Section: Discussionmentioning

confidence: 88%

“…The leaflet shape of the species, however, showed a clinal variation with that of V. unijuga vat. angustifolia Makino (Seok, 1997), and the individuals of two taxa were grouped together in the principal components analysis based on morphological characters (Seok and Choi, 1997). V. hifolia has been treated as a distinct species (Ohashi, 1982;Ohwi, 1984) or merged inlo V. unijuga (Franchet and Savatier, 1879;Kitamura and Murata, 1961 RAPD analysis is also in accord with the DNA sequence data of the ITS regions.…”

Section: Discussionmentioning

confidence: 99%

“…Odae National Park in eastern Korea by the characteristics of narrow linear leatlets and whitish flowers (Lee, 1982). This species, however, was not clearly distinguished from V. unijuga because the leaflet shapes and flower colors of the species fell within the variations of those in the latter (Seok and Choi, 1997).…”

Section: Yn Lee Which Are All Classified In the Sectionmentioning

confidence: 96%

“…Among the infraspecific taxa recognized in V. unijuga (Seok, 1997;Seok and Lee (1969) in this study. Because V. unijuga shows the greatest variation in external morphology at Mt.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 97%

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Taxonomic relationships in East AsianVicia species with unijugate leaves based on random amplified polymorphic DNA markers

Seok

Choi

1998

J. Plant Biol.

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Random amplified polymorphic DNA(RAPD) markers were investigated to clarify, the taxonomic positions of Vicia iinear~folia and V. b~folia, and to assess the genomic diversity among the 9 populations of V. un~iuga, each of which represents a geographical variation or infraspecific taxa in southern Korea. These species are characterized by unijugate leaves in East Asia and have been controversial as to infra-or interspecific classification. The polymorphic markers among the populations examined were observed for fifteen decamer primers. The degree of band sharing was used to calculate genetic similarity between populations, and a phenogram using UPGMA cluster analysis was generated based on the Dice similarity coefficient. The taxa studied were divided into two main groups and the populations of V. unijuga were all grouped together in the phenogram. The genetic similarities of V. unijuga were very high among the populations and did not show distinctions between the infraspecific taxa, although the populations of Mt. Odae and adjacent areas in eastern Korea were differen! from others of the species. V. linearifolia fell within the range of the genomic variation among the populations of V. un~iuga, while V. bifolia was grouped with V. venosa var. cuspidata having muitijugate leaves rather than V. un~]uga. The result from studying RAPD markers suggested that V. linearijolia should be integrated into V. un~]uga and that species with unijugate leaves of V. bifolia and V. un~iuga are polyphyletic.

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