Taxonomic relationships in East AsianVicia species with unijugate leaves based on random amplified polymorphic DNA markers

Abstract

Random amplified polymorphic DNA(RAPD) markers were investigated to clarify, the taxonomic positions of Vicia iinear~folia and V. b~folia, and to assess the genomic diversity among the 9 populations of V. un~iuga, each of which represents a geographical variation or infraspecific taxa in southern Korea. These species are characterized by unijugate leaves in East Asia and have been controversial as to infra-or interspecific classification. The polymorphic markers among the populations examined were observed for fifteen decamer primers. The degree of band sharing was used to calculate genetic similarity between populations, and a phenogram using UPGMA cluster analysis was generated based on the Dice similarity coefficient. The taxa studied were divided into two main groups and the populations of V. unijuga were all grouped together in the phenogram. The genetic similarities of V. unijuga were very high among the populations and did not show distinctions between the infraspecific taxa, although the populations of Mt. Odae and adjacent areas in eastern Korea were differen! from others of the species. V. linearifolia fell within the range of the genomic variation among the populations of V. un~iuga, while V. bifolia was grouped with V. venosa var. cuspidata having muitijugate leaves rather than V. un~]uga. The result from studying RAPD markers suggested that V. linearijolia should be integrated into V. un~]uga and that species with unijugate leaves of V. bifolia and V. un~iuga are polyphyletic.

Phylogenetic significance of stylar features in genus Vicia (Leguminosae): an analysis with molecular phylogeny

“…In these species, Vicia bifolia, V. chosenensis, and V. fauriae were recently added as new members of the section (Endo and Ohashi 1996b). V. angustepinnata was treated as a synonym of V. hirticalycina (Kwon and Choi 2001), and V. linearifolia is morphologically similar to V. unijuga (Lee 1996;Seok and Choi 1998). These two species, V. angustepinnata and V. linearifolia, were treated as members of the section Vicilla in the present study.…”

Taxonomic entities of two Korean plant taxa: Vicia bifolia (Fabaceae) and Cyperus compressus (Cyperaceae)

“…The two taxa mentioned above differ by having long petioles over 0.5-1.5 cm in length, having no bracts or those that are very small, approximately 1 mm in length, and that easily fall off. Random amplified polymorphic DNA data and an internal transcribed spacer sequencing analysis by Seok and Choi (1998) also revealed that Japanese V. bifolia was grouped with V. venosa var. cuspidata Maxim.…”