Targeting and genotyping RGAs in a mappingpopulation of the AA genome of wild Arachis

Leal-Bertioli, S. C. de M.; Guimarães, P. M.; Bertioli, David J.

doi:10.12702/1984-7033.v07n02a10

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Two primer pairs that amplify specific NBS encoding regions, both sequence confirmed [ 23 ], and one dominant SCAR marker derived from a bacterial artificial chromosome clone (Ad25F09-1; [ 24 ]) identified as containing a NBS encoding region by filter hybridization with clone S1_A_36 (Genbank ref. AY157808 ; [ 21 ]) were used for genotyping.…”

Section: Methodsmentioning

confidence: 99%

Identification of candidate genome regions controlling disease resistance in Arachis

et al. 2009

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Background: Worldwide, diseases are important reducers of peanut (Arachis hypogaea) yield. Sources of resistance against many diseases are available in cultivated peanut genotypes, although often not in farmer preferred varieties. Wild species generally harbor greater levels of resistance and even apparent immunity, although the linkage of agronomically un-adapted wild alleles with wild disease resistance genes is inevitable. Marker-assisted selection has the potential to facilitate the combination of both cultivated and wild resistance loci with agronomically adapted alleles. However, in peanut there is an almost complete lack of knowledge of the regions of the Arachis genome that control disease resistance.

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Section: Methodsmentioning

confidence: 99%

Identification of candidate genome regions controlling disease resistance in Arachis

et al. 2009

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“…The common motifs within the NBS domain that are highly conserved across plant species have provided opportunities for isolating resistant gene analogues (RGAs) by using PCR based approach with degenerate primers in a large number of plant species such as soybean, potato, lettuce, cereals, sugar beet, rape, cotton, peanut and chestnut rose [12][13][14][15][16][17][18][19][20][21]. Therefore, to detect polymorphisms between the two phenotypes of MYMIV-reactions we have designed degenerate primers from the conserved, characterized motifs of the functional NBS domains of R gene encoded in silico translated proteins and their homologues (RGH) in the family Fabaceae [22,23] known as RGA primers.…”

Section: Introductionmentioning

confidence: 99%

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

et al. 2010

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Mungbean Yellow Mosaic India Virus (MYMIV) belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean (Vigna radiata), urdbean (Vigna mungo) and soybean (Glycine max). The disease is severe, critical, open spread and inflicts heavy yield losses annually. The objective of this study is to develop molecular markers linked to MYMIV-resistance to facilitate genotyping of urdbean and mungbean germplasms for MYMIV-reaction. Resistance-linked molecular markers were successfully developed from consensus motifs of other resistance (R) gene or R gene homologue sequences. Applying linked marker-assisted genotyping, plant breeders can carry out repeated genotyping throughout the growing season in absence of any disease incidence. Two MYMIV-resistance marker loci, YR4 and CYR1, were identified and of these two CYR1 is completely linked with MYMIV-resistant germplasms and co-segregating with MYMIV-resistant F₂, F₃ progenies of urdbean. The present study demonstrated that these two markers could be efficiently employed together in a multiplex-PCR-reaction for genotyping both V. mungo and V. radiata germplasms from field grown plants and also directly from the seed stock. This method of genotyping would save time and labour during the introgression of MYMIV-resistance through molecular breeding, as methods of phenotyping against begomoviruses are tedious, labour and time intensive.

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“…The wild relatives of peanut are a rich source of disease resistance genes, because they have high genetic diversity and have been selected during evolution in a range of environments and biotic stresses (Stalker 1997;Leal-Bertioli et al 2007). In this study, the AA genome A. stenosperma (accession V10309) was used because: it is resistant to M. arenaria (Proite et al 2007; is closely related to A. duranensis, the most probable A genome donor to cultivated peanut (Seijo et al 2004); forms fertile hybrids with apparently normal genetic behavior; and is a parent of a well characterized diploid mapping population for the A genome of Arachis (Moretzsohn et al 2005;Guimarães et al 2008;Bertioli et al 2009;Leal-Bertioli et al 2009).…”

Section: Introductionmentioning

confidence: 99%

A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria

Guimarães

Brasileiro

Proite

et al. 2010

Tropical Plant Biol.

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Peanut (Arachis hypogaea) is amongst the most important legume crops in the world. One of its main yield constraints is the root-knot nematode Meloidogyne arenaria. A number of wild Arachis species, including A. stenosperma, are resistant to nematodes, and are a potential source of new resistance alleles for cultivated peanut. Using in silico subtraction of ESTs and macroarray analysis, we identified genes differentially expressed in A. stenosperma roots during its resistance response to M. arenaria. The three most differentially expressed genes [Auxin Repressed Protein (AsARP), Cytokinin Oxidase (AsCKX) and Metallothionein Type 2 (AsMET2)] were further analyzed using northern-blot and showed distinct expression profiles in the resistant A. stenosperma and susceptible A. hypogaea, both after, and sometimes even before, challenge with nematodes. Of the three most differentially expressed genes, AsARP and AsCKX are potentially involved in plant hormonal balance, and AsMET2 may be related to the reactive oxygen reaction triggered by the hypersensitive response (HR).

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Targeting and genotyping RGAs in a mappingpopulation of the AA genome of wild Arachis

Abstract: -Peanut is an important legume crop. Although it has high levels of morphological diversity

Cited by 3 publications

References 22 publications

Identification of candidate genome regions controlling disease resistance in Arachis

Identification of candidate genome regions controlling disease resistance in Arachis

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria

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