A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria

Guimarães, P. M.; Brasileiro, Ana Cristina Miranda; Proite, Karina; Araújo, Ana Cláudia Guerra; Leal‐Bertioli, Soraya C. M.; Pic‐Taylor, Aline; Silva, Felipe Rodrigues da; Morgante, Carolina Vianna; Ribeiro, Simone da Graça; Bertioli, David J.

doi:10.1007/s12042-010-9056-z

Cited by 29 publications

(26 citation statements)

References 67 publications

(82 reference statements)

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“…However, throughout the assay, an increase in expression was observed, and 79% of the genes were upregulated at 9 DAI. These results agree with the expression profiles reported during root-knot nematode infection in Arachis (Guimarães et al 2010;Tirumalaraju et al 2011) and other plants, such as rice (Kyndt et al 2012), Arabidopsis (Jammes et al 2005;Barcala et al 2010), tomato (Portillo et al 2013) and cowpea (Das et al 2010). These studies reported gene repression or constant expression at early stages of nematode-plant interaction, followed by a trend towards an increasing number of upregulated genes in both compatible and incompatible interactions.…”

Section: Gene Expression Profilessupporting

confidence: 90%

“…Basic helix-loop-helix (bHLH) proteins constitute one of the largest TFs families in Arabidopsis (Heim et al 2003). In this study, the significant upregulation of an A. stenosperma homolog (AsbHLH) in infected roots corroborates previous macroarray data ( Guimarães et al 2010). Some of these bHLH TFs form clusters regulating specific jasmonic acid (JA)-dependent responses, which modulate processes including defence against pathogens (Fernandez-Calvo et al 2011).…”

Section: Transcriptional Regulationsupporting

confidence: 88%

“…Recently, it was shown in Vitis vinifera that resveratrol, in addition to its classical role as an antimicrobial phytoalexin, is an important regulator for initiation of HR-related cell death (Chang et al 2011). In the present study, a variant homolog of A. stenosperma RS gene AsRS1, previously analysed using macroarray by Guimarães et al (2010), named AsRS2, showed strong upregulation in the three time points analysed, coinciding with the occurrence of HR in A. stenosperma. Therefore, we suggest that AsRS2 is involved in the HR response of A. stenosperma to M. arenaria and is one of our premium candidates for overexpression in transgenic peanut to enhance nematode resistance.…”

Section: Pathogen Defence Responsesupporting

confidence: 53%

“…Twelve genes were chosen based on our previous work (Proite et al 2008;Guimarães et al 2010) and our wild Arachis transcripts database. The remaining genes were selected considering their role in root-knot resistance in plants (Jammes et al 2005;Tirumalaraju et al 2011;Kyndt et al 2012) and A. stenosperma homologous sequences identified using BLAST tools (Altschul et al 1990).…”

Section: Candidate Gene Selectionmentioning

confidence: 99%

“…In cultivated peanut infected with M. arenaria, Tirumalaraju et al (2011) showed several genes were differentially expressed in resistant and susceptible cultivars. Likewise, the analysis of expressed sequence tags (ESTs) derived from wild A. stenosperma revealed a number of differentially expressed genes between inoculated and control plants; these being related to hormonal balance, defence and alleviation of oxidative stress triggered by HR (Proite et al 2007;Guimarães et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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A survey of genes involved in Arachis stenosperma resistance to Meloidogyne arenaria race 1

Morgante

Brasileiro

Roberts³

et al. 2013

Functional Plant Biol.

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Abstract. Root-knot nematodes constitute a constraint for important crops, including peanut (Arachis hypogaea L.). Resistance to Meloidogyne arenaria has been identified in the peanut wild relative Arachis stenosperma Krapov. & W. C. Greg., in which the induction of feeding sites by the nematode was inhibited by an early hypersensitive response (HR). Here, the transcription expression profiles of 19 genes selected from Arachis species were analysed using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), during the early phases of an A. stenosperma-M. arenaria interaction. Sixteen genes were significantly differentially expressed in infected and non-infected roots, in at least one of the time points analysed: 3, 6, and 9 days after inoculation. These genes are involved in the HR and production of secondary metabolites related to pathogen defence. Seven genes encoding a resistance protein MG13, a helix-loop helix protein, an ubiquitin protein ligase, a patatin-like protein, a catalase, a DUF538 protein, and a resveratrol synthase, were differentially expressed in all time points analysed. Transcripts of two genes had their spatial and temporal distributions analysed by in situ hybridisation that validated qRT-PCR data. The identification of candidate resistance genes involved in wild peanut resistance to Meloidogyne can provide additional resources for peanut breeding and transgenic approaches.

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Section: Gene Expression Profilessupporting

confidence: 90%

Section: Transcriptional Regulationsupporting

confidence: 88%

Section: Pathogen Defence Responsesupporting

confidence: 53%

Section: Candidate Gene Selectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A survey of genes involved in Arachis stenosperma resistance to Meloidogyne arenaria race 1

Morgante

Brasileiro

Roberts³

et al. 2013

Functional Plant Biol.

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Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

Burow¹,

Leal‐Bertioli²,

Simpson³

et al. 2013

Translational Genomics for Crop Breeding

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Peanut is the second-most important legum e grown worldwide. Cultivated peanut is a disomic tetraploid, 2n-4 x -40, with limited genetic diversity due to a genetic bottleneck in formation o f the polyploid from ancestors A. duranensis and A. ipaensis. Consequently, resistance_to biotic stresses is limited in the cultigen; however, w ild species possess strong resistances. Transfer o f these re sistances is hindered by differences o f ploidy, but production o f synthetic amphidiploids, coupled with use o f m olecular markers, enables efficient gene transfer. Marker maps have been made from interspecific crosses, and SSR-based maps from cultivated parents have been developed recently. At least 410 resistance gene analogues have been identified. The first markers for biotic stress tolerance were for root-knot nematode resistance and introgressed from one A. cardenasii chromosome. These and improved markers have been used for marker-assisted backcrossing, contributing to release o f three cultivars. Additional QTLs have been identified since.Early and late leafspots cause significant yield losses worldwide, and resistance depends on multiple genes. U sing interspecific populations, five resistance QTLs for early leafspot were identified using greenhouse inoculations, and five QTLs for late leafspot were identified using detached leaf assays. U sing cultivated species populations, 28 QTLs were identified for LLS resistance; all but one w ere minor QTLs; the major QTL was donated by an interspecific introgression line parent. Rust often occurs alongside leafspots, and rust resistance was characterized as one major QTL, plus several smaller QTLs. Marker-assisted backcrossing o f this major QTL has been performed into different populations. QTLs for resistance to other biotic stresses have been identified, namely to groundnut rosette virus, Sclerotinia blight, afiatoxin contamination, aphids, and tomato spotted w ilt virus. Marker-assisted breeding is still in early stages, and develop ment o f more rapid and inexpensive markers from transcriptome and genom e sequencing is expected to accelerate progress.

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Validation of resistance to root‐knot nematode incorporated in peanut from the wild relative Arachis stenosperma

et al. 2021

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Peanut (Arachis hypogaea L.) root-knot nematode (PRKN) Meloidogyne arenaria is a very destructive pathogen to which most peanut cultivars are highly susceptible. Current peanut cultivars rely on a single locus for PRKN resistance incorporated from the wild relative A. cardenasii Krapov. & W.C. Greg., that could be overcome as a result of the emergence of new nematode populations. Thus, new sources of resistance are continually needed. A new and strong resistance has been found in the wild diploid relative, A. stenosperma Krapov. & W.C. Greg. Arachis stenospermaderived quantitative trait loci (QTL) were described on chromosomes A02 and A09 and reduced nematode development by up to 98.2%. In order to validate these resistance segments, this study screened for PRKN resistance in BC 2 F 1 lines and correlated with molecular genotypes. Here, six BC 2 F 1 s carrying chromosome introgressions in A02 and/or A09, showed strong resistance while one line was susceptible. Both phenotype and genotype data allowed us to validate and delineate the chromosomal segments in chromosomes A02 and A09 to ∼8.5Mbp and ∼6.5Mbp regions on the bottom of each, respectively. Within the QTL on A02 and top middle of both chromosomes A02 and A09 there are R-gene clusters, often implicated in pathogen resistance. We have provided validation of these key resistance QTL that can be used to inform breeding via marker selection and insights into the functional basis of resistance provided by the wild peanut relative A. stenosperma.Abbreviations: BC, backcrossed lines; DAI, days after inoculation; FDR, false discovery rate; J 2 , second-stage juveniles; J 3 /J 4 , third/forth-stage juveniles; MABC, marker-assisted backcrossing; PRKN, peanut root-knot nematode; QTL, quantitative trait loci; SNP, single nucleotide polymorphismThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria

Cited by 29 publications

References 67 publications

A survey of genes involved in Arachis stenosperma resistance to Meloidogyne arenaria race 1

A survey of genes involved in Arachis stenosperma resistance to Meloidogyne arenaria race 1

Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

Validation of resistance to root‐knot nematode incorporated in peanut from the wild relative Arachis stenosperma

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