Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

Abstract

Mungbean Yellow Mosaic India Virus (MYMIV) belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean (Vigna radiata), urdbean (Vigna mungo) and soybean (Glycine max). The disease is severe, critical, open spread and inflicts heavy yield losses annually. The objective of this study is to develop molecular markers linked to MYMIV-resistance to facilitate genotyping of urdbean and mungbean germplasms for MYMIV-reaction. Resistance-linked molecular markers were successfully developed from consensus motifs of other resistance (R) gene or R gene homologue sequences. Applying linked marker-assisted genotyping, plant breeders can carry out repeated genotyping throughout the growing season in absence of any disease incidence. Two MYMIV-resistance marker loci, YR4 and CYR1, were identified and of these two CYR1 is completely linked with MYMIV-resistant germplasms and co-segregating with MYMIV-resistant F₂, F₃ progenies of urdbean. The present study demonstrated that these two markers could be efficiently employed together in a multiplex-PCR-reaction for genotyping both V. mungo and V. radiata germplasms from field grown plants and also directly from the seed stock. This method of genotyping would save time and labour during the introgression of MYMIV-resistance through molecular breeding, as methods of phenotyping against begomoviruses are tedious, labour and time intensive.

Re-evaluation of the Mungbean [Vigna radiata (L.) Wilczek] Genotypes for Resistance to Mungbean Yellow Mosaic Virus (MYMV) under Screen-House Conditions

“…However, only some of the genotypes in the intermediate categories showed slight variable responses for MYMV disease incidence under screen-house conditions. Forced feeding method has also been used earlier by several workers (Basak et al, 2004;Kundagrami et al, 2009;Maiti et al, 2011) in order to confirm the nature of reaction of parents and segregating mapping populations against MYMV disease. The screening for MYMV disease incidence resulted in the identification of two resistant genotypes namely IPM 02-14 and PDM 139 and a highly susceptible genotype, LGG 450.…”

Tropical Food Legumes

“…A SCAR marker linked to MYMV resistance gene was identified with a distance of 6.8 cm (Souframanien & Gopalakrishna, 2006). Two marker loci, YR4 and CYR1 (CYR1 was completely linked with MYMIV resistance), were employed in multiplex PCR reaction to screen quickly and reliably urdbean germplasm and breeding lines for resistance to MYMV (Maiti, Basak, Kundagrami, Kundu, & Pal, 2011). A single dominant gene was shown to govern resistance in a cross that involved mungbean genotypes DPU 88-31 (resistant) Â AKU 9904 (susceptible).…”

Identification of mungbean lines with tolerance or resistance to yellow mosaic in fields in India where different begomovirus species and different Bemisia tabaci cryptic species predominate

“…RAPD, SCAR and ISSR markers for MYMV resistance have been reported, but not successfully used in breeding, probably due to poor reproducibility and low degree of linkage (Selvi et al 2006;Somta et al 2009;Souframanien and Gopalakrishna 2006). Two resistance markers for MYMIV were identified based on conserved R-gene sequences, and successfully applied for germplasm screening (Maiti et al 2011). A field screening in Pakistan identified three out of 146 recombinant in-bred lines (RILs) developed in Thailand as tolerant since they displayed milder disease symptoms despite high disease pressure (Akhtar et al 2009).…”