Targeted thermal therapy with genetically engineered magnetite magnetosomes@RGD: Photothermia is far more efficient than magnetic hyperthermia

Sangnier, Anouchka Plan; Prévéral, Sandra; Curcio, Alberto; Silva, Amanda; Lefèvre, Chistopher T.; Pignol, David; Lalatonne, Yoann; Wilhelm, Claire

doi:10.1016/j.jconrel.2018.04.036

Cited by 116 publications

(125 citation statements)

References 52 publications

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“…As expected, the SAR values obtained for the randomly oriented bacteria diminish, although they still compare well with most of the SAR values reported in the literature for iron oxide nanoparticles . We also have to take into account the possibility that SAR values of bacteria could diminish after internalization in cancer cells, as has been frequently reported for magnetic nanoparticles . Therefore, it is important to test the capability of the bacteria as hyperthermia agents in in vitro experiments.…”

Section: Resultsmentioning

confidence: 61%

“…The actual mass of magnetite incorporated by the cells after 24 h of incubation was of around 8–10 pg magnetite/cell, a very small percentage of what was initially added to the cell culture (≈50 µg mL −1 ). Unfortunately, with such low magnetite internalization we could not measure any SAR value with our hyperthermia systems in order to test if cell internalization had an impact on the heating efficiency of bacteria as it has been reported for isolated magnetosomes …”

Section: Resultsmentioning

confidence: 99%

“…and their elimination (drug delivery, mechanical nanoactuation, magnetic hyperthermia, photothermia, etc.) [9,[13][14][15][16] Despite all this research, we are still, unfortunately, far from a standardized clinical implementation of magnetic nanoparticles. There are several issues and limitations that are intrinsic to the nature of these nanoparticles, such as their lack of self-propelled mobility and environment sensing mechanisms, their tendency to become agglomerated inside the tumors, their low targeting efficiency when injected intravenously, the difficulties for removing them from the body after the treatment is finished, etc.…”

Section: Magnetic Hyperthermiamentioning

confidence: 99%

“…[38] We also have to take into account the possibility that SAR values of bacteria could diminish after internalization in cancer cells, as has been frequently reported for magnetic nanoparticles. [16,33] Therefore, it is important to test the capability of the bacteria as hyperthermia agents in in vitro experiments.…”

Section: Magnetic Hyperthermiamentioning

confidence: 99%

“…Unfortunately, with such low magnetite internalization we could not measure any SAR value with our hyperthermia systems in order to test if cell internalization had an impact on the heating efficiency of bacteria as it has been reported for isolated magnetosomes. [16] To determine the potential cytotoxic effect that the bacteria could cause in cancer cells, we checked cell viability after 24 h and 48 h of the bacteria uptake. This was done using flow cytometry and Hoechst/propidium iodide staining to discriminate between living and dead cells.…”

Section: Bacterial Interaction With Cancer Cells and In Vitro Hyperthmentioning

confidence: 99%

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Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents

Gandia

Gandarias

Rodrigo

et al. 2019

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Magnetotactic bacteria are aquatic microorganisms that internally biomineralize chains of magnetic nanoparticles (called magnetosomes) and use them as a compass. Here it is shown that magnetotactic bacteria of the strain Magnetospirillum gryphiswaldense present high potential as magnetic hyperthermia agents for cancer treatment. Their heating efficiency or specific absorption rate is determined using both calorimetric and AC magnetometry methods at different magnetic field amplitudes and frequencies. In addition, the effect of the alignment of the bacteria in the direction of the field during the hyperthermia experiments is also investigated. The experimental results demonstrate that the biological structure of the magnetosome chain of magnetotactic bacteria is perfect to enhance the hyperthermia efficiency. Furthermore, fluorescence and electron microscopy images show that these bacteria can be internalized by human lung carcinoma cells A549, and cytotoxicity studies reveal that they do not affect the viability or growth of the cancer cells. A preliminary in vitro hyperthermia study, working on clinical conditions, reveals that cancer cell proliferation is strongly affected by the hyperthermia treatment, making these bacteria promising candidates for biomedical applications.

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: Magnetic Hyperthermiamentioning

confidence: 99%

Section: Magnetic Hyperthermiamentioning

confidence: 99%

Section: Bacterial Interaction With Cancer Cells and In Vitro Hyperthmentioning

confidence: 99%

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Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents

Gandia

Gandarias

Rodrigo

et al. 2019

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The Size, Shape, and Composition Design of Iron Oxide Nanoparticles to Combine, MRI, Magnetic Hyperthermia, and Photothermia

Freis

Cotin

Perton

et al. 2021

Magnetic Nanoparticles in Human Health and Medicine

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Endosomal Confinement of Gold Nanospheres, Nanorods, and Nanoraspberries Governs Their Photothermal Identity and Is Beneficial for Cancer Cell Therapy

et al. 2020

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Gold nanoparticles can act as photothermal agents to generate local tumor heating and subsequent depletion upon laser exposure. Herein, photothermal heating of four gold nanoparticles and the resulting induced cancer cell death are systematically assessed, within extra‐ or intracellular localizations. Two state‐of‐the‐art gold nanorods are compared with small nanospheres (single‐core) and nanoraspberries (multicore). Heat generation is measured in water dispersion and in cancer cells, using lasers at wavelengths of 680, 808, and 1064 nm, covering the entire range used in photothermal therapy, defined as near infrared first (NIR‐I) and second (NIR‐II) windows, with NIR‐II offering more tissue penetration. When dispersed in water, gold nanospheres provide no significant heating, gold nanorods are efficient in NIR‐I, and only gold nanoraspberries are still heating in NIR‐II. However, in cells, due to endosomal confinement, all nanoparticles present an absorption red‐shift translating visible and NIR‐I absorbing nanoparticles into effective NIR‐I and NIR‐II nanoheaters, respectively. The gold nanorods then become competitive with the multicore nanoparticles (nanoraspberries) in NIR‐II. Similarly, once in cells, gold nanospheres can be envisaged for NIR‐I heating. Remarkably, nanoraspberries are efficient nanoheaters, whatever the laser applied, and the extra‐ versus intra‐cellular localization demonstrates treatment versatility.

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Targeted thermal therapy with genetically engineered magnetite magnetosomes@RGD: Photothermia is far more efficient than magnetic hyperthermia

Cited by 116 publications

References 52 publications

Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents

Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents

The Size, Shape, and Composition Design of Iron Oxide Nanoparticles to Combine, MRI, Magnetic Hyperthermia, and Photothermia

Endosomal Confinement of Gold Nanospheres, Nanorods, and Nanoraspberries Governs Their Photothermal Identity and Is Beneficial for Cancer Cell Therapy

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