Targeted expression of insecticidal hybrid SN19 gene in potato leads to enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata Say) and tomato leafminer (Tuta absoluta Meyrick)

Ahmed, Hussein Abdullah Ahmed; Onarici, Selma; Bakhsh, Allah; Akdoğan, Güray; Karakoç, Ömer Cem; Özcan, Sancar Fatih; Aydin, Gulsum; Aasım, Muhammad; Ünlü, Levent; Sancak, Cengiz; Naimov, Samir; Özcan, Sebahattin

doi:10.1007/s11816-017-0453-8

Cited by 16 publications

(13 citation statements)

References 34 publications

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“…In the present study, cry1Ac expression was also studied under the control of wound specific AoPRI promoter. Expression levels of cry1Ac were recorded more under 35S promoter as compared to AoPR1 that are contrary to the results earlier obtained Ahmed et al (2017) and Anayol et al (2016).…”

Section: Discussioncontrasting

confidence: 99%

“…These transgenic plants are becoming important components of integrated pest management worldwide (Kos et al 2009). According to the literature, use of 35S CaMV promoter has been the first choice of all researchers to express foreign protein genes constitutively in commercialized transgenic crops; however, the targeted expression of foreign gene proteins has immense significance in transgenic technology to address the concerns of unnecessary protein production, and to avoid extra metabolic burden on plants (Ahmed et al 2017;Bakhsh et al 2016). It has already been established that cry1 toxin shows efficacy against lepidopterans (Bravo et al 2007); however, there are very few reports about their efficacy against CPB (Yüceer and Kayım 2012).…”

Section: Introductionmentioning

confidence: 99%

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An effective pest management approach in potato to combat insect pests and herbicide

Amiri

Bakhsh

2019

3 Biotech

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Insect pests and weeds incur significant yield losses to potato crop worldwide. The increasing crop losses provide impetus for the development of pest management strategy that is equally effective against insect pests and weeds. In the present study, a molecular approach was used to develop transgenic potato lines (cv. Marabel) effective against Colorado potato beetle (Leptinotarsa decemlineata Say), potato tuber moth (Phthorimaea operculella Z.) and Basta ® application. Agrobacterium tumefaciens strain EHA105 harboring binary vector pTF101.1 containing cry1Ac gene under the control of 35S and AoPR1 promoters was used to infect leaf discs and internodal explants. Phosphinothrincin was used at optimal concentration (2 mg/l) for the screening of primary transformants. The standard molecular assays exhibited gene integration and expression in putative transgenics. Real-time data revealed up to ninefold high cry1Ac transcript levels, whereas cry protein amount was estimated to 0.4 ppm in primary transformants. The analysis of first tuber progeny showed proper integration cry1Ac and bar genes in subsequent progeny. The transgenic plants also showed tolerance to the application of Basta ®. The efficacy of cry1Ac was evaluated by allowing larvae of Colorado potato beetle (CPB) and potato tuber moth (PTM) to feed on transgenic plants. Results revealed appreciable mortality levels of different larval instars of CPB (20-100%) and PTM (50-100%). Overall, our results exhibit the potential of these transgenic lines to be used in a potato breeding program with the purpose to control insect pests and weeds.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An effective pest management approach in potato to combat insect pests and herbicide

Amiri

Bakhsh

2019

3 Biotech

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“…Most importantly, different strains of B. thuringienesis produce a variety of δ-endotoxins or crystal proteins with specific host range. The various Bt endotoxin gene(s) encoding resistance against lepidopteran and coleopteran pests has been incorporated in potatoes as evident from scientific literature available (Amiri and Bakhsh, 2019, Ahmed et al, 2017, Mohammed et al, 2016Mi et al, 2015;Veale et al, 2012;Kumar et al, 2010;Davidson et al, 2004;Chakrabarti et al, 2000;Perlak et al, 1993). There are also attempts to transfer genes of plant origin (proteinase inhibitors, amylose inhibitors and lectins) that encode resistance against these notorious insects (Table-1).…”

Section: Insect Resistant Potatoesmentioning

confidence: 99%

“…The use of light inducible promoter restricted cry1Ab expression to aerial parts of transgenic potatoes and resulted in enhanced resistance against PTM. Using SN19 gene, under the control of constitutive (35S) and wound inducible promoter (AoPR1), Ahmed et al (2017) were able to develop transgenic potato lines equally effective against CPB and PTM.…”

Section: Insect Resistant Potatoesmentioning

confidence: 99%

“…Both the adults and larva of CPB feed on potato leaves, thus reducing yield significantly and can even kill plants. CPB is notorious pest for development of resistance to insecticides over short periods of time (Kamenova et al, 2008;Ahmed et al, 2017). Similarly, different species of nematodes are also very important pests of potato and cause significant losses in potato (Ali et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Bakhsh¹,

Dangol²,

Naeem³

et al. 2020

THE JAPS

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Potato is one of the most important food crops in terms of annual production and food security worldwide. The crop is affected by several types of biotic stresses, e.g. insects, viruses, fungus, nematodes and weeds, which are the prominent limiting factors for its production. The conventional breeding methods in potato have been associated with limitations; none of the present day commercial cultivar has built-in resistance against biotic stresses. There is strong need for the development of new resistant potato varieties to cope against biotic stresses using non-classical approaches in combination with classical methods. The scientific literature suggests the contribution of modern biotechnological techniques for the development of transgenic potato lines resistant against insects and diseases. The present comprehensive review describes different genetic engineering approaches for the development of transgenic potatoes resistant to insects, weeds, nematodes, fungus and viruses by fellow researchers worldwide. It also gives an insight into modern technologies, e.g. RNAi and CRISPR-Cas9, which have emerged recently and can be implemented in the development of biotic stress resistant potato cultivars.

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Plant Recombinant Gene Technology for Pest Control in the Twenty-First Century: From Simple Transgenesis to CRISPR/Cas

Dubovskiy,

Grizanova,

Gerasimova

2024

Gene Editing in Plants

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Targeted expression of insecticidal hybrid SN19 gene in potato leads to enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata Say) and tomato leafminer (Tuta absoluta Meyrick)

Cited by 16 publications

References 34 publications

An effective pest management approach in potato to combat insect pests and herbicide

An effective pest management approach in potato to combat insect pests and herbicide

GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Plant Recombinant Gene Technology for Pest Control in the Twenty-First Century: From Simple Transgenesis to CRISPR/Cas

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