Tanshinone IIA ameliorates seawater exposure-induced lung injury by inhibiting aquaporins (AQP) 1 and AQP5 expression in lung

Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition

“…Antitumor activities, cardioprotective, and neuroprotective effects of Tan ⅡA have been reported in many earlier studies [13e15]. Notably, Tan ⅡA has also been proven to effectively mitigate seawater exposure-induced lung injuries such as lung edema and vascular leakage in rodents [16,17], suggesting that Tan ⅡA has a protective activity in pulmonary diseases. Fu et al [18] have found that Tan ⅡA blocks EMT in MCF-7 and HCC1973 breast cancer cell lines Q5 .…”

Tanshinone IIA therapeutically reduces LPS-induced acute lung injury by inhibiting inflammation and apoptosis in mice

“…We also showed that pretreatment with TIIA greatly decreased the production of pro-inflammatory cytokines in both LPS-treated mice and cultured macrophages, which most likely correlated with the inhibition of HIF-1α expression by TIIA [9] . Moreover, we further indicated that pretreatment with TIIA could significantly attenuate the severity of ALI in seawater-challenged rats through the depression of macrophage migration inhibitory factor [12] , aquaporins (AQP) 1 and AQP5 expression [11] and apoptosis [13] and up-regulation of Na + ,K + -ATPase activity [10] .…”

“…TIIA may exert a series of biochemical effects, such as anti-oxidant and antiinflammatory effects. Our previous work has demonstrated that TIIA was able to alleviate ALI induced by lipopolysaccharide (LPS) [8,9] and seawater exposure [10][11][12][13] , indicating that TIIA may be a potential agent to treat ALI.…”

Aquaporin Changes in Compound 48/80 Induced Inflammatory Sublaryngeal Edema in Rat

“…The primers for b-actin, AQP1, AQP3, AQP4, and AQP5 were as the following. 16,17 b-actin primers: forward primer 5 0 -ATCATCTTTGA GACCTTCAACA-3 0 , reverse primer 5 0 -CATCTCTTGCTC GAAGTCCA-3 0 , PCR product length 270bp; AQP1 primers: forward primer 5 0 -GGACAATGTGAAGGTGTCACTGGC-3 0 , reverse primer 5 0 -CCAGAAAATCCAGTGGTTTGAGAA-3 0 , PCR product length 499bp; AQP3 primers: forward primer 5 0 -GTTCCGTGGCTCAAGTGGTGC-3 0 , reverse primer 5 0 -GCCCATGGAGGTCCCAATGAC-3 0 , PCR product length 500bp; AQP4 primers: forward primer 5 0 -CCATTAACTG GGGTGGCTCAG-3 0 , reverse primer 5 0 -AACTGCAGGGCC AAAGGATCG-3 0 , PCR product length 500bp; AQP5 primer: forward primer 5 0 -CAGCACTCAAGTGGCCCTCGGCTC-3 0 , reverse primer 5 0 -AAAGATCGGGCTGGGTTCATGGAA-3 0 , PCR product length 478bp. Data were recorded and analyzed by using ABI Prism 7300SDS software.…”