Tailored Glycopolymers:  Controlling the Carbohydrate−Protein Interaction Based on Template Effect

Nagahori, Noriko; Nishimura, Shin‐Ichiro

doi:10.1021/bm0000438

Cited by 48 publications

(47 citation statements)

References 16 publications

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“…This includes: free-radical polymerization (FRP), 45,47,[49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64] living anionic polymerization, 65 ring-opening polymerization (ROP), 66,67 ringopening metathesis polymerization (ROMP), 44,68-74 and controlled/living free-radical polymerization (CLRP) which, encompasses nitroxide mediated controlled free-radical polymerization (NMP), 75-83 atom transfer radical polymerization (ATRP), [84][85][86][87][88][89][90][91][92][93] and reversible addition-fragmentation chain transfer (RAFT) polymerisation. 16,46,[94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110][111]<...>…”

Section: Glycopolymers From the Polymerization Of Glycomonomersmentioning

confidence: 99%

Synthesis of glycopolymers and their multivalent recognitions with lectins

2010

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Synthetic carbohydrate ligands -also widely known as glycopolymers -are known to undergo numerous recognition events when interacting with their corresponding lectins. Interactions are greatly enhanced due to the multivalent character displayed by the large number of repeating carbohydrate units along the polymers (pendant glycopolymers); therefore, resulting what is called the ''glycocluster effect''. Moreover, the strength and the availability of these multivalent recognitions can be tuned via the architecture of the glycopolymers. Hence, understanding the mechanistic interactions between the types of lectins (plant, animal, toxin and bacteria) with their synthetic ligands is crucial. This review focuses on the synthesis of pendant glycopolymers via various synthetic pathways (free radical polymerization, NMP, RAFT, ATRP, cyanoxyl mediated polymerization, ROP, ROMP and post-polymerization modification) and their interactions with their respectively lectins.

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Section: Glycopolymers From the Polymerization Of Glycomonomersmentioning

confidence: 99%

Synthesis of glycopolymers and their multivalent recognitions with lectins

2010

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“…30 On the whole, the glycopolymersubstituted substrate showed suitable properties for biosensing applications. Biosensing with a glycopolymer thin layer will be investigated in a later section.…”

Section: Spr Analyses Of Glycopolymer-protein Interactionsmentioning

confidence: 99%

Biological specific recognition of glycopolymer- modified interfaces by RAFT living radical polymerization

Toyoshima

Oura

Fukuda

et al. 2009

Polym J

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Glycopolymers with a-galactose (a-Gal) and a-mannose (a-Man) were synthesized by means of living radical polymerization with a reversible addition-fragment chain transfer reagent, and the thin-layer formation of glycopolymers was investigated in terms of protein recognition abilities. Thiol-terminated glycopolymers formed a thin layer of about 2.5 nm in thickness on a gold substrate, and the glycopolymer thin layer showed specific interaction with sugar recognition proteins (lectins and Shiga toxins (Stxs)). The interactions were highly specific, and the signal-to-noise ratio of protein recognition was greater than 16. Glycopolymer-substituted gold nanoparticles (GNPs) also showed biorecognition abilities and protein-specific aggregation. The protein recognition abilities of the GNPs were also analyzed. The glycopolymer-substituted GNPs were utilized for signal amplification of surface plasmon resonance (SPR) to detect protein-saccharide recognition. The glycopolymer with a-Gal showed a strong interaction with Stxs according to SPR measurements, suggesting a possible application of a-Gal-substituted GNPs in Stx-1 biosensing.

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“…For example, MIPs able to recognise specific compounds of cell surface, especially oligosaccharides or lectins, may be suitable for targeting the DDS to specific tissues or cells, increasing the residence time at the absorption site and providing an intimate contact to the absorptive tissue. Lectin-imprinted polymers have recently been prepared in aqueous solutions using the ability of this glycoprotein to reversibly bind certain saccharidic functional monomers [103]. The affinity of each lectin for specific mucosal surfaces of the gastrointestinal, respiratory, and urogenital tract may serve as a basis for the development of lectin-mediated drug delivery systems of poorly absorbable drugs by opening endocytic pathways [104].…”

Section: A View To the Futurementioning

confidence: 99%