TACI-ligand interactions are required for T cell activation and collagen-induced arthritis in mice

Wang, Hua; Marsters, Scot A.; Baker, Thad; Chan, Betty; Lee, Wyne P.; Fu, Lihua; Tumas, Daniel B.; Yan, Minhong; Dixit, Vishva M.; Ashkenazi, Avi; Grewal, Iqbal S.

doi:10.1038/89782

Cited by 200 publications

(114 citation statements)

References 31 publications

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“…Assays were performed by a previously reported method [21]. Briefly, antigen-induced T cell adhesion was quantified by using a colorimetric assay described for measuring intracellular succinate dehydrogenase content with MTT [3(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] [22].…”

Section: Methodsmentioning

confidence: 99%

B Cell-Activating Factor Is a Novel Diagnosis Parameter for Asthma

Kang¹,

Yoon²,

Ahn

et al. 2006

Int Arch Allergy Immunol

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Background: Asthma is a life-threatening immediate-type allergic disease. B cell-activating factor (BAFF) is a key regulator of B lymphocyte development and is required to generate and maintain the mature B cell pool. Objectives: To investigate the level of BAFF in the serum of asthma patients and the role of BAFF on T cells. Methods: The BAFF level was measured by enzyme-linked immunosorbent assay. Peripheral blood mononuclear cells (PBMC) from asthma patients were analyzed by flow cytometry. T8.1 cells were used to test the role of BAFF on T cell-antigen-presenting cell (APC) conjugate formation. Results: The BAFF level in patient serum was elevated relative to normal serum. Immunoglobulin E (IgE) concentration and the percentage of CD3+ T and CD19+ B cells vary according to the serum BAFF level. Patients with high BAFF and high IgE (group II) and those with high BAFF and low IgE (group III) show a high ratio of CD3+ T to CD19+ B cells, and the opposite is seen for patients with low BAFF and high IgE (group I) and those with low BAFF and low IgE (group IV). The addition of BAFF increased PBMC proliferation and T cell-APC conjugate formation. BAFF concentration in serum decreased after treatment with antiasthmatic drugs including glucocorticoids and immunosuppressants. Conclusion: These findings suggest that the serum BAFF level is high in both IgE-mediated asthma and non-IgE-mediated asthma and extend our knowledge about the fact that BAFF may play a stimulatory role on the proliferation of T cells. Thus, BAFF could be a parameter to monitor the severity of asthma symptoms.

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Section: Methodsmentioning

confidence: 99%

B Cell-Activating Factor Is a Novel Diagnosis Parameter for Asthma

Kang¹,

Yoon²,

Ahn

et al. 2006

Int Arch Allergy Immunol

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“…It has been shown that BLyS plays roles in the process of inflammation-associated lymphoproliferation and GC formation in the rheumatoid synovitis [22]. Serum BLyS levels were also reportedly elevated in collagen-induced arthritis (CIA) mouse model and AA rat model [29,33], with BLyS antagonism TACI-Ig having both preventive and therapeutic effects [37,38]. Zhang et al previously reported that elevated serum level of BLyS is closely correlated with profoundly increased anti-collagen type II autoantibody production in CIA mice [33].…”

Section: Discussionmentioning

confidence: 96%

Expression and effects of B-lymphocyte stimulator and its receptors in T cell-mediated autoimmune arthritis

Cao

Sun

Jia

et al. 2015

International Immunopharmacology

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“…(6,22) In an RA model of mice, TACI-Fc also reduces the tissue damage and prevents the progression of the disease. (23) A MAb, Lymphostat-B (Human Genome Sciences, Inc.), against human BLyS protein is being tested in a clinical trial for SLE.…”

Section: Discussionmentioning

confidence: 99%

Preparation and Characterization of a Monoclonal Antibody Against Human B Lymphocyte Stimulator

Sun

Li²,

Sun³

et al. 2006

Hybridoma

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B lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family. It is required for B cell development. Deregulation of BLyS was involved in the pathogenesis of B cell-related autoimmune diseases and multiple myeloma. To prepare monoclonal antibodies (MAbs) against BLyS, cDNA encoding soluble BLyS (sBLyS) was first amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with specific primers, and then inserted into a prokaryotic expression vector pET-30a. Right recombinant plasmid was expressed in Escherichia coli strain BL21(DE3), purified by nickel affinity chromatography. Isolated sBLys was used as an antigen to immunize mice. Splenocytes of one immunized mouse were fused with NS- 1. Hybridomas secreting antibodies against sBLyS were identified by ELISA. One positive clone was selected to produce antibody by injecting the hybridoma into the peritoneal cavity of mice. After collecting ascites, the antibody was purified by protein A affinity chromatography. Western blot and immunoflourescence demonstrated that the antibody could bind recombinant sBLyS and genuine membrane-bound BLyS (mBLyS) on U937. This MAb can be used as a detecting reagent to analyze the serum level of BLyS in patients with autoimmune diseases and the expression profile of mBLyS on multiple myeloma cells.

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TACI-ligand interactions are required for T cell activation and collagen-induced arthritis in mice

Cited by 200 publications

References 31 publications

B Cell-Activating Factor Is a Novel Diagnosis Parameter for Asthma

B Cell-Activating Factor Is a Novel Diagnosis Parameter for Asthma

Expression and effects of B-lymphocyte stimulator and its receptors in T cell-mediated autoimmune arthritis

Preparation and Characterization of a Monoclonal Antibody Against Human B Lymphocyte Stimulator

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