T Cells Activation and Interferon-γ/Nitric Oxide Production during Behçet Disease: A Study in Algerian Patients

Djaballah-Ider, Fatmazohra; Chaib, Samia; Belguendouz, Houda; Talbi, Djameleddine; Touil-Boukoffa, Chafia

doi:10.3109/09273948.2012.671882

Cited by 14 publications

(5 citation statements)

References 10 publications

(18 reference statements)

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“…Proinflammatory cytokines seem to be responsible for the enhanced inflammatory response in BD [ 14 , 15 ]. In our previous studies, we reported the overproduction of either Th1 (IL-12, IFN- γ ), Th2 (IL-4), or Treg (IL-10) cytokines during the different stages of the disease [ 16 – 19 ]. In addition, we observed an increase in inflammatory markers production (IL-8 and nitric oxide) during Behçet uveitis.…”

Section: Introductionmentioning

confidence: 99%

Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism

Belguendouz

Lahmar-Belguendouz

Messaoudene

et al. 2015

International Journal of Inflammation

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Aim and Methods. In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics. Cultures were performed with or without the control or patients plasma. Subsequent treatment contained anticytokines (IL-6, TGF-β), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4). Culture supernatants were harvested after 24 h of incubation. NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods. Results. Higher urea levels were found in patients' plasma compared to the control's (P < 0.05). NOS modulators induced inverted production profiles for NO and urea (P < 0.05). Their results differed depending on the clinical findings (P < 0.05). It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (P < 0.05). Conclusion. Our results suggest that arginases can compete with NOS2 for L-arginine during Behçet disease. Both enzymes are regulated by environmental cytokines and substrate availability. Furthermore, it seems that NOS/arginase balance is dependent on clinical expression.

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Section: Introductionmentioning

confidence: 99%

Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism

Belguendouz

Lahmar-Belguendouz

Messaoudene

et al. 2015

International Journal of Inflammation

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“…Pro-inflammatory cytokines seem to be responsible for the enhanced inflammatory response in BD [14,15]. In our previous studies, we reported the overproduction of either Th1 (IL-12, IFN-␥), Th2 (IL-4) or Treg (IL-10) during the different stages of the disease [16][17][18][19]. In addition, we observed an inflammatory markers production (IL-8 and nitric oxide) increase in during Behçet uveitis.…”

Section: Introductionmentioning

confidence: 60%

In vivo and in vitro IL-18 production during uveitis associated with Behçet disease: Effect of glucocorticoid therapy

Belguendouz

Messaoudene

Lahmar-Belguendouz

et al. 2015

Journal Français d'Ophtalmologie

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“…Djaballah-Ider et al in a study on 34 patients with BD showed that IFN-γ is involved in the inflammatory and pathogenic processes of BD by stimulating the production of No (25).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of IFN-Gamma and HSP70 Level in the Saliva of Behcet’s Disease Patients With Active Oral Lesions

Eslami¹,

Ghavidel²,

Khabbazi³

et al. 2019

Avicenna J Dent Res

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Introduction: Behcet's disease (BD) is a multi-systemic inflammatory disorder. Evaluating the production of cytokines such as interferon gamma and biomarkers such as heat shock protein-70 (HSP70)is an important way to study the pathogenesis and development of BD. This study aimed to compare the salivary level of interferon gamma and HSP70 between patients infected with BD and healthy individual. Methods: This case-control study was performed on 35 patients with Behcet's syndrome and 70 healthy individuals as the control group, who were selected from those referring to the Department of Oral Medicine of Tabriz University of Medical Sciences. The levels of interferon gamma and HSP70 were measured in the whole unstimulated saliva through enzyme-linked immunosorbent assay (ELIZA). In order to compare the quantitative variables between two groups, independent samples t-test or its nonparametric equivalent, Mann-Whitney U test, was used in SPSS software version 16.0. In this study, a P value less than 0.05 was considered statistically significant. Results: There was no significant difference between the study groups in terms of age and gender, as well as salivary interferon gamma and HSP70 levels. Interferon gamma level was 15.16 ± 3.38 pg/mg in the case group and 5.27 ± 1.21 pg/mg in the control group, and salivary HSP70 level was found to be 45.50 ± 17 ng/mL and 19.5 ± 5.2 ng/mL in the case and control groups, respectively. Conclusions: The results of this study showed that interferon gamma and HSP70 levels in patients with Behcet's syndrome are high and can be evaluated as an important tool for the treatment and evaluation of disease development in future studies.

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T Cells Activation and Interferon-γ/Nitric Oxide Production during Behçet Disease: A Study in Algerian Patients

Cited by 14 publications

References 10 publications

Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism

Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism

In vivo and in vitro IL-18 production during uveitis associated with Behçet disease: Effect of glucocorticoid therapy

Evaluation of IFN-Gamma and HSP70 Level in the Saliva of Behcet’s Disease Patients With Active Oral Lesions

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