Systemic responses of weaned rats to spermine against oxidative stress revealed by a metabolomic strategy

Liu, Guangmang; Fang, Tingting; Yan, Tao; Jia, Gang; Zhao, Hua; Chen, Xiaoling; Wu, Caimei; Wang, Jing

doi:10.1039/c4ra09975c

Cited by 18 publications

(20 citation statements)

References 48 publications

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“…The obtained results reveal that piglets supplied with spermine have lower serum TG level in comparison to the control group, which indicates that supplementation with spermine may affect lipid metabolism. However, such a result is inconsistent with the results of Liu et al (2014a), who reported that spermine significantly increased the TG levels in weaned rats under stressful conditions. In the Liu's experiment, rats received spermine supplementation and intraperitoneally diquat injection; on the other hand in the present study piglets were only supplemented with spermine (no stress was induced).…”

Section: Discussioncontrasting

confidence: 87%

“…Supplementation with an appropriate dose of spermine is correlated with the biochemical and physiological changes in the blood. Spermine affects glucose and protein metabolism by increasing glucose and triglyceride levels and decreasing blood urea nitrogen trend in rats (Liu et al, 2014a). However, no information is available on the effects of spermine supplementation and time of its duration on the changes in clinical chemistry in pigs.…”

Section: Introductionmentioning

confidence: 99%

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Effects of spermine supplementation on blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in piglets

Fang¹,

Jia²,

Zhao³

et al. 2019

J. Anim. Feed Sci.

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The effects of spermine supplementation and extended spermine supplementation on blood biochemical parameters, serum amino acid profile and ileum expression of amino acid transporters in piglets were examined in this study. Piglets were fed adequate nutrient intake supplemented or not with spermine (0.4 mmol/kg body weight) for 7 h or 3, 6 or 9 days. Spermine supplementation significantly increased aspartate aminotransferase activity and albumin content, but decreased triglyceride level in serum (P < 0.05). There was a significant increase in levels of arginine, histidine, isoleucine, leucine, threonine, valine, alanine, asparagine, serine, proline, taurine and tyrosine (P < 0.05) in the spermine-treated groups. Spermine significantly increased the mRNA levels of SLC1A1, SLC1A5, SLC7A1, SLC7A7, SLC7A9, SLC5A1, SLC6A19 and SLC15A1 in ileum (P < 0.05). The extended spermine supplementation exerted different effects on the blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in comparison to the 7-h group (P < 0.05). In summary, spermine supplementation can affect biochemical parameters and amino acid profile in serum and may enhance the ileum absorption of amino acids by increasing the expression of amino acid transporters in piglets.

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Section: Discussioncontrasting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Effects of spermine supplementation on blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in piglets

Fang¹,

Jia²,

Zhao³

et al. 2019

J. Anim. Feed Sci.

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“…40 Numerous factors, such as weaning, environmental factors and infection, can induce oxidative stress, resulting in serious economic losses during livestock production. 41,42 Natural antioxidants, such as COS, may be used to relieve oxidative stress in livestock production. 43 MDA is the most familiar breakdown product of lipid peroxidation and its level is frequently used as a direct marker of lipid oxidative damage caused by ROS.…”

Section: Discussionmentioning

confidence: 99%

New insights into the role of chitosan oligosaccharide in enhancing growth performance, antioxidant capacity, immunity and intestinal development of weaned pigs

Wan

Jiang

et al. 2017

RSC Adv.

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“…In the literature, spermine has been implicated in diverse biological and physiological events, such as in regulating replication, transcription, translation and post‐translational modification (Mandala, Mandal, Johansson, Orjalo, & Hee Park, ); controlling protein synthesis and cell membrane transporter (Igarashi & Kashiwagi, ); modulating ion channel activities (Pegg, ); interacting with intracellular messengers (Igarashi & Kashiwagi, ); changing intestinal morphology (Fang, Jia, et al., ); and promoting immune response (Pegg, ). Spermine can also change systemic metabolic processes of the body through affecting lipid metabolism, energy metabolism, amino acid metabolism, cell membrane metabolism and gut microbiota metabolism (Liu, Fang, Yan, Jia, Zhao, Chen, et al., , Liu, Fang, Yan, Jia, Zhao, Huang et al., , Liu et al., ). Supplementation with spermine in breast milk can accelerate the early presence of splenic B cells in suckling rats (Pérez‐Cano, González‐Castro, Castellote, Franch, & Castell, ), but the effect of spermine intake on the spleen and thymus of pigs remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Roles of spermine in modulating the antioxidant status and Nrf2 signalling molecules expression in the thymus and spleen of suckling piglets—new insight

Cao

Jia

et al. 2017

Animal Physiology Nutrition

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SummaryThis study aimed to investigate the effects of spermine and extended spermine administration on the antioxidant status and expression of NF-E2-related factor 2 (Nrf2) signalling molecules in the thymus and spleen in suckling piglets. One half of eighty 12-day-old suckling piglets obtained sufficient nutrient intake supplemented with spermine (0.4 mmol/kg body weight), and another half received restricted nutrient intake supplemented with physiological saline in equal doses once a day for 7 hr or 3, 6 or 9 days in pairs. Spermine supplementation and its extended duration significantly decreased malondialdehyde (MDA) and protein carbonyl (PC) contents (p < .05), but markedly improved antisuperoxide anion (ASA), antihydroxyl radical (AHR), catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities (p < .05) and glutathione (GSH) content (p < .05) in the thymus and spleen. Additionally, realtime PCR analysis showed that spermine administration and extended spermine intake reduced Kelch-like ECH-associated protein 1 (Keap1) gene transcription and enhanced SOD1, GPx1, CAT, glutathione reductase (GR) and Nrf2 mRNA levels of the thymus and spleen (p < .05), and also improved GST gene expression in the thymus (p < .05).Notably, the spermine-supplemented time for the optimal effects of suckling piglet was determined to be 6 days. Collectively, the current study suggested that spermine supplementation and extended spermine administration could protect the health of the thymus and spleen from early weaning by enhancing the antioxidant status and regulating the expression of antioxidant-related signalling molecules. K E Y W O R D Santioxidant status, signalling molecule, spermine, spleen, suckling piglet, thymus | INTRODUCTIONThe suckling period is known as a crucial phase of growth and development that mammals naturally experience. In commercial pig production, the suckling piglet is generally weaned early to obtain excellent economic profit. At this time, multiple biological stress responses are caused by early weaning, and weaning stress is highly prominent (Wang, Zeng, et al., 2016). Weaning stress can contribute to numerous biological processes, including enhanced disease susceptibility and metabolic disorders (Yin et al., 2013(Yin et al., , 2014, impaired intestinal histomorphology and functions Zhu, Zhao, Chen, & Xu, 2012), reduced feed intake and suppressedgrowth (Campbell, Crenshaw, & Polo, 2013). Furthermore, weaning stress also exerts affirmative effects on generating excessive reactive oxygen species (ROS) in a normal amount of the body (Yin et al., 2014), which may further lead to oxidative stress and changes in many antioxidant enzymes in the antioxidant defence systems, such as superoxide dismutase, catalase and glutathione peroxidase (Han, Shuvaev, & Muzykantov, 2011). Suitable therapies and nutritional support (e.g., L-arginine, N-carbamylglutamate and putrescine) can provide beneficial effects...

show abstract

Systemic responses of weaned rats to spermine against oxidative stress revealed by a metabolomic strategy

Cited by 18 publications

References 48 publications

Effects of spermine supplementation on blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in piglets

Effects of spermine supplementation on blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in piglets

New insights into the role of chitosan oligosaccharide in enhancing growth performance, antioxidant capacity, immunity and intestinal development of weaned pigs

Roles of spermine in modulating the antioxidant status and Nrf2 signalling molecules expression in the thymus and spleen of suckling piglets—new insight

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