Effects of spermine supplementation on blood biochemical parameters, amino acid profile and ileum expression of amino acid transporters in piglets

Fang, Tingting; Jia, Gang; Zhao, Heling; Chen, X.; Wu, Caimei; Xue, Bine; Cai, Jingyi; Tian, Gang; Wang, J.; Liu, G.

doi:10.22358/jafs/114433/2019

Cited by 1 publication

(1 citation statement)

References 29 publications

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“…Although Put changed the SCL36A1 and SLC1A1 gene, it did not affect the AA levels in the serum of weanling pigs. As Fang et al. (2019) described, supplementation of spermine, which could be converted from Put, enhanced the AA transporters mRNA abundances in the ileum of piglets during d 3 to 9 after weaning at 12 d old.…”

Section: Discussionmentioning

confidence: 60%

Serum biochemical parameters and amino acids metabolism are altered in piglets by early-weaning and proline and putrescine supplementations

Wang

Xiao

et al. 2021

Animal Nutrition

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The study was to investigate the effect of early-weaning stress and proline (Pro) and putrescine (Put) supplementations on serum biochemical parameters and amino acids (AA) metabolism in suckling and post-weaning pigs. Blood and small intestinal mucosa were harvested from suckling piglets at 1, 7, 14, and 21 d of age and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age, as well as from piglets received oral administration of Pro and Put from 1 to 14 d old. In suckling piglets, the serum glucose, albumin and total cholesterol levels were increased ( P < 0.05) with increasing age, whereas the serum globulin, urea nitrogen (BUN), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels were lowered ( P < 0.05). The concentrations of most serum AA and the AA transporters related gene expressions were highest in 7-d-old piglets ( P < 0.05), whereas the phosphorylation status of the mammalian target of the rapamycin (mTOR) signaling pathway in the small intestine increased in piglets from 1 to 21 d old ( P < 0.05). Weaning at 14 d old increased ( P < 0.05) the BUN and triglycerides levels in serum, as well as jejunal solute carrier family 7 member 6 ( SLC7A6 ), ileal SLC36A1 and SLC1A1 mRNA abundances at d 1 or 3 post-weaning. Weaning also inhibited ( P < 0.05) the phosphorylation levels of mTOR and its downstream ribosomal protein S6 kinase 1 (S6K1) and 4E-binding protein-1 (4EBP1) in the small intestine of weanling pigs. Oral administration of Put and Pro decreased ( P < 0.05) serum ALP levels and increased ( P < 0.05) intestinal SLC36A1 and SLC1A1 mRNA abundances and mTOR pathway phosphorylation levels in post-weaning pigs. Pro but not Put treatment enhanced ( P < 0.05) serum Pro, arginine (Arg) and glutamine (Gln) concentrations of weaning-pigs. These findings indicated that early-weaning dramatically altered the biochemical blood metabolites, AA profile and intestinal mTOR pathway activity, and Pro and Put supplementations improved the AA metabolism and transportation as well as activated the intestinal mTOR pathway in weanling-pigs. Our study has an important implication for the broad application of Pro and Put in the weaning transition of piglets.

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Section: Discussionmentioning

confidence: 60%