Systemic Profiles of microRNAs, Redox Balance, and Inflammation in Lung Cancer Patients: Influence of COPD

Abstract

Lung cancer (LC) risk increases in patients with chronic respiratory diseases (COPD). MicroRNAs and redox imbalance are involved in lung tumorigenesis in COPD patients. Whether systemic alterations of those events may also take place in LC patients remains unknown. Our objectives were to assess the plasma levels of microRNAs, redox balance, and cytokines in LC patients with/without COPD. MicroRNAs (RT-PCR) involved in LC, oxidized DNA, MDA-protein adducts, GSH, TEAC, VEGF, and TGF-beta (ELISA) were quantified in plasma samples from non-LC controls (n = 45), LC-only patients (n = 32), and LC-COPD patients (n = 91). In LC-COPD patients compared to controls and LC-only, MDA-protein adduct levels increased, while those of GSH decreased, and two patterns of plasma microRNA were detected. In both LC patient groups, miR-451 expression was downregulated, while those of microRNA-let7c were upregulated, and levels of TEAC and TGF-beta increased compared to the controls. Correlations were found between clinical and biological variables. A differential expression profile of microRNAs was detected in patients with LC. Moreover, in LC patients with COPD, plasma oxidative stress levels increased, whereas those of GSH declined. Systemic oxidative and antioxidant markers are differentially expressed in LC patients with respiratory diseases, thus implying its contribution to the pathogenesis of tumorigenesis in these patients.

Chronic Obstructive Pulmonary Disease: The Present and Future

“…Monitoring markers of oxidative stress and miR genotyping for precision-based chemotherapy [10] Iron-deficient COPD IV iron replacement Monitoring hepcidin as a marker for non-anaemic iron deficiency [23] Antimicrobial-resistant COPD Targeted antibiotics based on culture sensitivities Use of colour charts to determine commencement of antibiotics [14] Key-LABA: long-acting β-agonists; LAMA: long-acting muscarinic antagonists; ICS: inhaled corticosteroids; HRQoL: health-related quality of life; CFTR: cystic fibrosis transmembrane receptor; miR: microRNA; VTE: venous thromboembolism; NIV: noninvasive ventilation; OSA/ORRF: obstructive sleep apnoea/obesity-related respiratory failure; COPD: chronic obstructive pulmonary disease; IV: intravenous.…”

“…The authors identify an evidence gap in the expression of microRNA in cancer cells with and without established COPD, which a study by Qin et al successfully fills [ 10 ]. Qin et al isolated serologic microRNA and oxidative stress markers from patients with and without lung cancer, some of whom had COPD.…”

Respiratory and Peripheral Muscle Weakness and Body Composition Abnormalities in Non-Cystic Fibrosis Bronchiectasis Patients: Gender Differences

“…Extrapulmonary manifestations are common among patients with chronic respiratory diseases, including those with bronchiectasis [ 5 , 6 , 7 , 8 ]. The analysis of skeletal muscle dysfunction and sarcopenia of patients with chronic obstructive pulmonary disease (COPD) have been a matter of research in multiple previous investigations [ 9 , 10 , 11 , 12 , 13 , 14 ].…”