Abstract:Intraperitoneal administration of nanocurcumin can be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.
“…After this stage, the Bcl-2 prohibits the apoptosis that triggers the cellular proliferation. 18 Our RT-PCR analyses showed that in MRSA/CNP/ HAMLET group, caspase 3, Bcl-2, and p43 expressions were increased. Thus, it could be concluded that curcumin nanoparticles in presence of HAMLET could enhance the cellular proliferation by upregulating the caspase 3, Bcl-2, and p43 expressions.…”
Section: Discussionmentioning
confidence: 66%
“…Antioxidant activities including superoxide dismutase (SOD), total nitric oxide synthase (tNOS), malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), glutathione peroxidase (GPO), glutathione reductase (GSHRd), glutathione S-transferase (GST) analyses, isolation of DNA from tissue, and cDNA hydrolysis with formic acid measurement of 8-hydroxy-2′deoxyguanosine (8-OH Gua) were performed based on methods described by others. 18 Histological Preparation and Quantitative Morphometric Studies. The tissue samples were taken on 7, 14, and 21 days after surgery from periphery of the wound along with normal skin and fixed in 10% buffered formalin, dehydrated and embedded in paraffin wax, sectioned at 5 µm, and stained with hematoxylin and eosin (H&E) and Masson's trichrome stains.…”
Accurately orchestrated course of events normally observed in healing are not followed in diabetic wounds, and bacterial colonization/infection further messes up the process. Novel therapeutic options for treatment of infections caused by multidrug-resistant Staphylococcus aureus are urgently needed. HAMLET (human α-lactalbumin made lethal to tumor cells) has been reported to be able to sensitize bacterial pathogens to traditional antimicrobial agents. The aim was to assess the wound healing activity of curcumin nanoparticles in diabetic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) sensitized with HAMLET. Fifty male rats were randomized into 5 groups of 10 animals each. In CONTROL group, 0.1-mL sterile saline 0.9% solution was added to the wounds with no infection. In MRSA group, the wounds were infected with MRSA and only treated with 0.1-mL sterile saline 0.9% solution. In MRSA/HAMLET group, infected wounds were treated with HAMLET (100 µg). In MRSA/CNP group, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL curcumin nanoparticles. In MRSA/CNP/HAMLET group, animals with infected wounds were treated with topical application of 0.1 mL solution of curcumin nanoparticles (1 mg/mL) and HAMLET (100 µg). All test formulations were applied for 10 days, twice a day, starting from first treatment. Microbiological examination; planimetric, biochemical, histological, and quantitative morphometric studies; immunohistochemical staining for angiogenesis; determination of hydroxyproline levels; and reverse transcription polymerase chain reaction for caspase 3, Bcl-2, and p53 showed that there was significant difference between animals in MRSA/CNP/HAMLET group compared with other groups ( P < .05). Curcumin nanoparticles improved diabetic wounds infected with MRSA sensitized with HAMLET and had the potential to offer more attention to this safer agent for topical use in infected diabetic wounds.
“…After this stage, the Bcl-2 prohibits the apoptosis that triggers the cellular proliferation. 18 Our RT-PCR analyses showed that in MRSA/CNP/ HAMLET group, caspase 3, Bcl-2, and p43 expressions were increased. Thus, it could be concluded that curcumin nanoparticles in presence of HAMLET could enhance the cellular proliferation by upregulating the caspase 3, Bcl-2, and p43 expressions.…”
Section: Discussionmentioning
confidence: 66%
“…Antioxidant activities including superoxide dismutase (SOD), total nitric oxide synthase (tNOS), malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), glutathione peroxidase (GPO), glutathione reductase (GSHRd), glutathione S-transferase (GST) analyses, isolation of DNA from tissue, and cDNA hydrolysis with formic acid measurement of 8-hydroxy-2′deoxyguanosine (8-OH Gua) were performed based on methods described by others. 18 Histological Preparation and Quantitative Morphometric Studies. The tissue samples were taken on 7, 14, and 21 days after surgery from periphery of the wound along with normal skin and fixed in 10% buffered formalin, dehydrated and embedded in paraffin wax, sectioned at 5 µm, and stained with hematoxylin and eosin (H&E) and Masson's trichrome stains.…”
Accurately orchestrated course of events normally observed in healing are not followed in diabetic wounds, and bacterial colonization/infection further messes up the process. Novel therapeutic options for treatment of infections caused by multidrug-resistant Staphylococcus aureus are urgently needed. HAMLET (human α-lactalbumin made lethal to tumor cells) has been reported to be able to sensitize bacterial pathogens to traditional antimicrobial agents. The aim was to assess the wound healing activity of curcumin nanoparticles in diabetic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) sensitized with HAMLET. Fifty male rats were randomized into 5 groups of 10 animals each. In CONTROL group, 0.1-mL sterile saline 0.9% solution was added to the wounds with no infection. In MRSA group, the wounds were infected with MRSA and only treated with 0.1-mL sterile saline 0.9% solution. In MRSA/HAMLET group, infected wounds were treated with HAMLET (100 µg). In MRSA/CNP group, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL curcumin nanoparticles. In MRSA/CNP/HAMLET group, animals with infected wounds were treated with topical application of 0.1 mL solution of curcumin nanoparticles (1 mg/mL) and HAMLET (100 µg). All test formulations were applied for 10 days, twice a day, starting from first treatment. Microbiological examination; planimetric, biochemical, histological, and quantitative morphometric studies; immunohistochemical staining for angiogenesis; determination of hydroxyproline levels; and reverse transcription polymerase chain reaction for caspase 3, Bcl-2, and p53 showed that there was significant difference between animals in MRSA/CNP/HAMLET group compared with other groups ( P < .05). Curcumin nanoparticles improved diabetic wounds infected with MRSA sensitized with HAMLET and had the potential to offer more attention to this safer agent for topical use in infected diabetic wounds.
“…100 mg/kg curcumin 2. 1 mg/kg nano curcumin Intraperitoneal Group SSG: laparotomy only Group I: 3 h ischemia only Group I/R: 3 h ischemia and 3 h reperfusion Group I/C: 3 h ischemia only and 100 mg/kg curcumin Group I/R/C: 3 h ischemia, 3 h reperfusion and 100 mg/kg curcumin Group I/NC: 3 h ischemia only and 1 mg/kg nano curcumin Group I/R/NC: 3 h ischemia, 3 h reperfusion and 1 mg/kg nano curcumin -Nanocurcumin-treated animals showed significantly improved development of ischemia and reperfusion tissue injury compared with other groups -I/R/NC group showed significantly higher superoxide dismutase values, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase than other groups (p < 0.05) -I/R/NC group showed a significantly lower value of nitric oxide synthase, malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine levels compared with other Groups -No difference in all biochemical parameters in other groups [69] CBA female mice immunised with an extract from the ovaries of outbred albino mice to induce immune disease of the ovaries (model of autoimmune disease in women) Curcumin at 100 µg/g (four times a week) Intragastric -The percentage of oocytes in metaphases I and II: Immunised mice: the numbers of oocytes with dissolved germinal vesicle (metaphase I) and the formed polar body (metaphase II) decreased significantly Curcumin-treated mice: the level of oocytes in metaphases I and II increased significantly compared with the immunised group -The number of apoptotic and necrotic cells in the thymus, spleen and lymph node:…”
Section: Effect Of Curcumin On Ovarian Diseasesmentioning
confidence: 79%
“…Interestingly, in another rat model of ovarian ischemia–reperfusion injury, 1 mg/kg nano curcumin improved the oxidative status parameters but not 100 mg/kg. These results included significantly higher values of superoxide dismutase, total glutathione, GSH, glutathione reductase and glutathione S-transferase and substantially lower values of nitric oxide synthase, MDA, myeloperoxidase and 8-hydroxy-2 deoxyguanine levels [ 69 ]. The findings from these two studies showed that nanocurcumin at very low concentrations (1 mg/kg) produced significant improvements compared to the native curcumin (100 mg/kg and 200 mg/kg curcumin), which might be attributed to its high bioavailability and longer half-life.…”
Section: Effect Of Curcumin On Ovarian Diseasesmentioning
Curcumin is one of the main polyphenolic compounds in the turmeric rhizome. It possesses antioxidant, anti-inflammatory, anti-cancer, anti-arthritis, anti-asthmatic, anti-microbial, anti-viral and anti-fungal properties. This review aims to provide an overview of the potential health benefits of curcumin to treat female reproductive disorders, including polycystic ovary syndrome (PCOS), ovarian failure and endometriosis. Comprehensive information on curcumin was retrieved from electronic databases, which were MEDLINE via EBSCOhost, Scopus and Google Scholar. The available evidence showed that curcumin reduced the high level of androgen in PCOS. Studies in rodents suggest that curcumin resulted in the disappearance of cysts and the appearance of healthy follicles and corpora lutea. Furthermore, animal studies showed curcumin improved the overall function of the ovary in ovarian diseases and reversed the disturbance in oxidative stress parameters. Meanwhile, in vitro and in vivo studies reported the positive effects of curcumin in alleviating endometriosis through anti-inflammatory, anti-proliferative, anti-angiogenic and pro-apoptotic mechanisms. Thus, curcumin possesses various effects on PCOS, ovarian diseases and endometriosis. Some studies found considerable therapeutic effects, whereas others found no effect. However, none of the investigations found curcumin to be harmful. Curcumin clinical trials in endometriosis and ovarian illness are still scarce; thus, future studies need to be conducted to confirm the safety and efficacy of curcumin before it could be offered as a complementary therapy agent.
“…In damaged tissue, increase in the levels of p53 and caspase is observed to trigger apoptotic activity of the immune cells that results in the exclusion of the immune cells. Following this phase, the Bcl-2 inhibits the apoptotic activity that induces the proliferation of cells [ 34 ]. In the present study, analyses of RT-PCR indicated that in INFCTD-HMLT-CNM animals, expressions of caspase 3, Bcl-2, and p43 were amplified.…”
Objective. The aim of the present study was to investigate the effect of cinnamon nanoparticles (CNPs) on healing of wounds infected with methicillin-resistant Staphylococcus aurous with human alpha-lactalbumin made lethal to tumor cells sensitization in diabetic rats. Methods. We included fifty diabetic male rats and divided them into 5 groups. There were 10 rats in each group as follows: CONTROL group: we did not infect the CONTROL group. The wound was only covered with sterile saline 0.9% solution (0.1 mL). INFCTD group: in this group, the wounds were infected with MRSA and covered with sterile saline 0.9% solution (0.1 mL). INFCTD-HMLT group: in this group, the wounds were infected with MRSA and HAMLET (100 μg). INFCTD-CNM group: in this group, the wounds were infected with MRSA and 0.1 mL CNPs (1 mg/mL) were applied topically to wounds. INFCTD-HMLT-CNM group: in this group, the wounds were infected with MRSA, HAMLET (100 μg), and 0.1 mL CNPs (1 mg/mL). Results. Bacteriology, wound area reduction measurements, biochemistry, histomorphometrical studies, hydroxyproline levels, and reverse transcription polymerase chain reaction for caspase-3, Bcl-2, and p53 showed significant difference between rats in the INFCTD-HMT-CNM group in comparison with other groups (
P
<
0.05
). Conclusions. Accelerated healing of diabetic wounds infected with MRSA showed that local application of cinnamon nanoparticles along with HAMLET sensitization on S. aureus-infected wound could be taken into consideration.
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