The aim of this study was to assess the effect of locally administered prostaglandin E1 on peripheral nerve regeneration and functional recovery. Sixty male healthy white Wistar rats were divided into four experimental groups (n = 15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using silicone graft (SIL/PE) filled with 10 μL prostaglandin E1. In silicone graft group (SIL), the graft was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and regenerated nerve fibers were studied 4, 8 and 12 weeks after surgery. Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in SIL/PE than SIL group (p < 0.05). In immunohistochemistry, location of reactions to S-100 in SIL/PE was clearly more positive than that in SIL group. When loaded in a silicone graft, prostaglandin E1 improved functional recovery and morphometric indices of sciatic nerve. Local application of prostaglandin E1 improved functional recovery and morphometric indices of sciatic nerve.
Osteoarthritis is the most common type of arthritis and the major cause of chronic disability in musculoskeletal mobility in the elderly populations worldwide. In this study, the scaffold free autologous platelet rich plasma (PRP) in an experimental animal model of osteoarthritis (OA) by direct intra articular injection was used.Seventeen New Zealand white adult rabbits of both sexes were used. Osteoarthritis was induced by anterior cruciate ligament transection of knee joints. Eight weeks following operation, a single dose of (0.5 ml) Platelet Rich plasma (PRP) was delivered into the injured knee by direct intra articular injection. The control group received no treatment. The knees were examined on 12 and 16 weeks past from the initial operation. The healing status was investigated histopathologically.Pathologic assessment confirmed development of OA changes after 8 weeks which was advanced at 12 weeks in the control group. PRP treated Rabbits showed lower degree of cartilage degeneration, less osteophyte formation, and minimal subchondral sclerosis at 12 weeks following surgery compared to the control group. The PRP group had near to normal joint structure at 16 week postoperative interval. This study showed that the PRP could potentially be used for the treatment of osteoarthritis.
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