Systematic separation and purification of 18 antioxidants from Pueraria lobata flower using HSCCC target-guided by DPPH–HPLC experiment

Separation and purification of α-glucosidase inhibitors from Polygonatum odoratum by stepwise high-speed counter-current chromatography combined with Sephadex LH-20 chromatography target-guided by ultrafiltration–HPLC screening

“…Petroleum ether-ethyl acetate-methanol-water solvent system, providing a broad range of polarities by modifying volume ratios of four solvents, has been testified as be useful in purification of components in ethyl acetate fraction [29].…”

Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

“…DPPH-HPLC is a simple and rapid screening method that aids in the rapid selection of target components in complex extracts and has been widely used in many studies. [25][26][27] HSCCC is a unique separation technique based on the liquid-liquid partition chromatography without any solid support matrix. Therefore, it prevents the loss of sample observed in the conventional adsorption chromatography.…”

Separation of polyphenols from leaves of Malus hupehensis (Pamp.) Rehder by off-line two-dimensional High Speed Counter-Current Chromatography combined with recycling elution mode

“…Typically, it is extremely difficult to separate these compounds through just altering volume ratio of solvent system (Shi et al, 2012). One possible solution is to introduce complexation agents such as copper (Liu, Luo, & Kong, 2011) or silver ion (Wen et al, 2009) into solvent system.…”

“…Retention time (R t ) of a solute in HSCCC is directly decided by its distribution coefficient (K). Thus, it is common that compounds are co-eluted in one peak when implementing HSCCC in the separation of natural products (Shi et al, 2012). Longer HSCCC column might provide improved separation efficiency when there is little difference in K values.…”

“…Consequently, severe peak overlapping usually could be observed for complicated sample when implementing recycling HSCCC (R-HSCCC) separation (Shi et al, 2012). As we know, the crude extract of natural products is extremely complicated, which could probably contain from dozens to hundreds of compounds (Ma, Liang, Jiang, Wang, & Luo, 2012).…”