Comprehensive evaluation of the antioxidant capacity of <i>Perilla frutescens</i> leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Kwon, Shin Hwa; Wang, Zhiqiang; Hwang, Seung Hwan; Kang, Young‐Hee; Lee, Jae Yong; Lim, Soon Sung

doi:10.1080/10942912.2017.1318289

Cited by 29 publications

(39 citation statements)

References 53 publications

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“…These results indicated that corilagin (E) possessed the best DPPH• scavenging rate (71.8 ± 0.5%) and •OH inhibition rate (75.9 ± 0.3%), and protocatechuic acid (A) had the strongest chelation activity of ferrous ion (36.4 ± 0.7%). These results were consistent with the results reported before [19,20]. Meanwhile, proanthocyanidins C1 (F) exhibited the weakest capacity of scavenging DPPH radical and chelating ferrous ion, and quercetin (C) had a minimum •OH inhibition rate.…”

Section: Resultssupporting

confidence: 92%

HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects

Pan

et al. 2019

Molecules

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Longan (Dimocarpus longan Lour.) pericarp, the main by-product of aril and pulp processing, is abundant in phenolic compounds and worthy of further utilization. The present work firstly reported HPLC analysis and in vitro antioxidant evaluation of longan (cv. Shixia) pericarp-derived phenolics (LPPs), the purified longan pericarp extract (LPE), as well as their cytotoxic effect on lung cancer cell line, A549. The results indicated that the purified LPE had significant amounts of phenolics, with content of 57.8 ± 0.6 mg of gallic acid equivalents per gram of dry longan pericarp (mg GAE·g−1 DLP), which consisted of six phenolic compounds (A–F), including protocatechuic acid (A), isoscopoletin (B), quercetin (C), ellagic acid (D), corilagin (E), and proanthocyanidins C1 (F). Antioxidant assays showed that LPPs (10 μM) and LPE (1.0 mg·mL−1) had certain antioxidant activities, in which corilagin (E) possessed the best DPPH radical scavenging rate 71.8 ± 0.5% and •OH inhibition rate 75.9 ± 0.3%, and protocatechuic acid (A) exhibited the strongest Fe2+ chelating ability 36.4 ± 0.7%. In vitro cytotoxic tests suggested that LPPs had different effect on A549 cell line, in which corilagin (E) exhibited potent cytotoxicity with an IC50 value of 28.8 ± 1.2 μM. These findings were further confirmed by cell staining experiments.

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Section: Resultssupporting

confidence: 92%

HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects

Pan

et al. 2019

Molecules

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“…Therefore, it is important to characterize the extracts by a variety of antioxidant assays. [20] To the best of our knowledge, the antioxidant potentialities of MG extract have seldom been studied; Couladis et al [21] showed, by a method based on the peroxidation of arachidonic acid, that the methanol extract of MG was similar in activity to α-tocopherol. In the present work, four antioxidant assays were carried out.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…[23] Quenching of the ABTS •+ and DPPH • radicals The ABTS •+ and DPPH • assays, based on single-electron transfer mechanisms, are simple and common methods for evaluating antioxidant activities. [20] The IC 50 values indicate that the ethanol extract of MG is potent in neutralizing ABTS •+ (IC 50 , 30.5 ± 0.9 µg/mL) and DPPH • (IC 50 , 65.8 ± 2.4 µg/mL) radicals ( Table 1). These assays indicate a hydrogen and/or electron donation capacity of the MG polar extract, an important factor to protect biomolecules from ROS in susceptible biological and food systems.…”

Section: Inhibition Of Linoleic Acid Peroxidationmentioning

confidence: 99%

Phenolic profile and biological activities ofMicromeria graeca(L.) Benth. ex Rchb.

Brahmi

Guendouze

Hauchard

et al. 2017

International Journal of Food Properties

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The medicinal potential of the ethanol extract of Micromeria graeca (L.) Benth. ex Rchb., harvested in Algeria (MG extract), was evaluated by assessing in vitro its antioxidant, antibacterial, and antityrosinase properties. The total phenolic (= 430 ± 30 mg gallic acid equivalent/100 g of dry weight) and flavonoid (= 190 ± 10 mg quercetin equivalent/100 g of dry weight) contents were evaluated by the Folin-Ciocalteu and the aluminium chloride methods, respectively. Silica gel thin-layer chromatography revealed the presence of chlorogenic, caffeic, and rosmarinic acids and diosmin; the presence of these compounds was confirmed by reverse phase high-performance liquid chromatography. The IC 50 values indicate that the ethanol extract of M. graeca is highly potent in neutralizing the 2,2ʹ-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS •+ ; IC 50 = 30.5 ± 0.9 µg/mL) and the 2,2ʹ-diphenyl-1-picrylhydrazyl radicals (DPPH • ; IC 50 = 65.8 ± 2.4 µg/mL). The MG extract was also very active on a lipid peroxidation model (IC 50 = 23.4 ± 1.9 µg/mL). The superoxide quenching index values (electrochemically measured) were 332 ± 37 (AI 30) and 638 ± 53 µg/mL (AI 50). The studied extract showed a moderate tyrosinase inhibitory activity (IC 50 = 302 ± 62 µg/mL) and a low antibacterial effect while the combination of this extract with antibiotics restored the activities of cefotaxime and streptomycin on resistant Staphylococcus aureus and Pseudomonas aeruginosa.

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“…The antioxidant capacities of SOB and DSOB were assessed using electron transfer assays (DPPH and ABTS) and ROS scavenging assays (HO•, NO, and ONOO − ). The DPPH, ABTS, HO•, and NO scavenging assays were performed as reported by Kwon et al [ 19 ]. The ONOO − scavenging assay was carried out as reported by Hazra et al [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Defatted Seeds of Oenothera biennis as a Potential Functional Food Ingredient for Diabetes

Wang

Zuo

et al. 2021

Foods

Self Cite

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The defatted seeds of Oenothera biennis (DSOB) are a by-product of evening primrose oil production that are currently not effectively used. In this study, α-glucosidase inhibition, aldose reductase inhibition, antioxidant capacity, polyphenol composition, and nutritional value (carbohydrates, proteins, minerals, fat, organic acid, and tocopherols) of DSOB were evaluated using the seeds of Oenothera biennis (SOB) as a reference. DSOB was an excellent inhibitor of α-glucosidase (IC50 = 3.31 μg/mL) and aldose reductase (IC50 = 2.56 μg/mL). DSOB also showed considerable antioxidant capacities (scavenging of 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, nitric oxide, peroxynitrite, and hydroxyl radicals). DSOB was a reservoir of polyphenols, and 25 compounds in DSOB were temporarily identified by liquid chromatography coupled with electrospray ionization–quadrupole time of flight–mass spectrometry analysis. Moreover, the carbohydrate, protein, and mineral content of DSOB were increased compared to that of SOB. DSOB contained large amounts of fiber and low levels of sugars, and was rich in calcium and iron. These results imply that DSOB may be a potential functional food ingredient for diabetes, providing excellent economic and environmental benefits.

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Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Cited by 29 publications

References 53 publications

HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects

HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects

Phenolic profile and biological activities ofMicromeria graeca(L.) Benth. ex Rchb.

Defatted Seeds of Oenothera biennis as a Potential Functional Food Ingredient for Diabetes

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