Systematic degradation mechanism and pathways analysis of the immobilized bacteria: Permeability and biodegradation, kinetic and molecular simulation

Fu, Xinge; Wang, Huajun; Bai, Yu; Xue, Junmin; Gao, Yu; Hu, Shugang; Wu, Tongtong; Sun, Jingkuan

doi:10.1016/j.ese.2020.100028

Cited by 39 publications

(21 citation statements)

References 30 publications

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“…This is due to their ability to produce enzymes (metabolic components) that can absorb and neutralize the toxic nature of the waste [32][33][34]. Sponges are also able biodegrade aliphatic and aromatic hydrocarbon components, presumably because of the role of symbiotic bacteria or because of the metabolic components produced [35,36]. The biodegradation of PAHs and bio-adsorption of heavy metals have led to the marine sponge being identified with significant benefits in reducing pollutants and maintaining the quality of the marine environment [37,38].…”

Section: Introductionmentioning

confidence: 99%

Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

et al. 2021

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Every petroleum-processing plant produces sewage sludge containing several types of polycyclic aromatic hydrocarbons (PAHs). The degradation of PAHs via physical, biological, and chemical methods is not yet efficient. Among biological methods, the use of marine sponge symbiont bacteria is considered an alternative and promising approach in the degradation of and reduction in PAHs. This study aimed to explore the potential performance of a consortium of sponge symbiont bacteria in degrading anthracene and pyrene. Three bacterial species (Bacillus pumilus strain GLB197, Pseudomonas stutzeri strain SLG510A3-8, and Acinetobacter calcoaceticus strain SLCDA 976) were mixed to form the consortium. The interaction between the bacterial consortium suspension and PAH components was measured at 5 day intervals for 25 days. The biodegradation performance of bacteria on PAH samples was determined on the basis of five biodegradation parameters. The analysis results showed a decrease in the concentration of anthracene (21.89%) and pyrene (7.71%), equivalent to a ratio of 3:1, followed by a decrease in the abundance of anthracene (60.30%) and pyrene (27.52%), equivalent to a ratio of 2:1. The level of pyrene degradation was lower than that of the anthracene due to fact that pyrene is more toxic and has a more stable molecular structure, which hinders its metabolism by bacterial cells. The products from the biodegradation of the two PAHs are alcohols, aldehydes, carboxylic acids, and a small proportion of aromatic hydrocarbon components.

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Section: Introductionmentioning

confidence: 99%

Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

et al. 2021

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“…One H2O molecule comes out, and the pyrene structure changes to a penantroic carboxylic acid product. This process continues following the oxidation reaction process and the breakdown of the second and third rings until the final product is reached, namely a benzene carboxylic acid molecule in the form of cinnamate and a pinacol product [27,31,39,88].…”

Section: Discussionmentioning

confidence: 99%

“…These two types of bacteria have striking differences, not only on the source of isolates but also on different types, strains or characters, Gram groups, including different morphological and microscopic isolates. Thus it is suspected that there are differences in the degradation ability of PAH components, especially pyrene [39]. Comparison of growth rates between Bl and Sb bacteria on selective media with an incubation period of up to 30 days can be seen based on the optical density of the growth of the two types of bacteria measured at λmaks 600 nm [3,9], according to Figure 5.…”

Section: Microscopic and Morphological Analysismentioning

confidence: 99%

“…Deactivation of toxic aromatic elements using natural materials, such as microorganisms as biomaterials with the function of degrading aromatic components through the mechanism of destruction of the PAH aromatic ring structure by enzymatic-behaving substances produced by microorganisms, especially bacteria in response to habitats that are not conducive to life [12,[35][36][37][38] Bacteria can be found in almost all water, soil and air environments, including the sea. Bacteria in the marine can form independent communities, generally having specific characteristics and characteristics depending on their habitat [1,[39][40][41]. Bacterial communities living in marine areas are exposed to wastes such as heavy metals, PAHs, pesticides, and biphenyl polychloride [2,42,43].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Strength of Biodegradation of Pyrene between Symbiont Bacteria of Marine Sponges and Bacteria Isolated from Seawater

Marzuki¹,

Nisaa²,

Asaf³

et al. 2022

Preprint

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PAHs contaminants have toxic, carcinogenic, and even mutagenic properties. Screening bacteria from different sources capable of carrying out the biodegradation of PAHs is important for mapping and mobilization purposes and applying them to polluted hydrocarbon environments. The study aimed to compare the biodegradation power of two types of bacteria isolated from different sources against PAHs. The method applied is the interaction between bacterial suspension and pyrene contaminated waste for 30 days. Biodegradation products in organic compounds were analyzed using GC-MS and FTIR. The analysis results found several indications of the performance of bacterial biodegradation, namely: the aggressiveness of biodegradation of Bl bacteria against pyrene was relatively more dominant than Sb bacteria. Percentage of total bacterial biodegradation product type Sb (39.00 %), while the product of bacterial degradation type Bl (38.29 %); the biodegradation products of the test bacteria (Bl and Sb) were relatively similar to pyrene, in the form of alcohol and carboxylic acid organic compounds. It was concluded that there was no significant difference in biodegradation performance between Bl and Sb bacteria on pyrene. Both types of bacterial isolates from different sources can carry out the function of biodegradation of Pyrene.

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“…It is due to their ability to produce enzymes (metabolic components) that can absorb and neutralize the toxic nature of the waste [32][33][34]. Sponges also have the function of biodegradation of aliphatic and aromatic hydrocarbon components, presumably because of the role of symbiotic bacteria they have or because of the metabolic components produced [35,36]. The function of biodegradation of PAHs and bio-adsorption of heavy metals has earned the marine sponge the nickname of biota with dual functions and significant benefits in reducing pollutants and maintaining the quality of the marine environment [37,38].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium Against Anthracene and Pyrene

Marzuki¹,

Asaf²,

Paena³

et al. 2021

Preprint

View full text Add to dashboard Cite

Every petroleum processing industry produces sewage sludge containing several types of poly-cyclic aromatic hydrocarbon (PAHs) components. The degradation of PAH components by physical, biological and chemical methods is not efficient. The use of marine sponge symbiont bacteria is considered an alternative method in the degradation and reduction of PAHs com-pared to the previous method. This study aims to explore the potential and performance of a consortium of sponge symbiont bacteria in degrading anthracene and pyrene. There are three types of bacteria (Bacillus pumilus strain GLB197, Pseudomonas stutzeri strain SLG510A3-8, Acineto-bacter calcoaceticus strain SLCDA 976) were mixed to form a consortium. The interaction between the bacterial consortium suspension and PAH components was measured at 5-day intervals for 25 days. The biodegradation performance of bacteria on PAHs samples was determined based on five biodegradation parameters. The analysis results showed a decrease in the concentration of anthracene (21.89%) and pyrene (7.71%), equivalent to a ratio of 3: 1. The data was followed by a decrease in the abundance of anthracene (60.30%) and pyrene (27.52%), an equivalent ratio of 2: 1. The level of degradation of the pyrene component is lower than that of the anthracene compo-nent, presumably due to the higher toxicity of pyrene and the more stable molecular structure, making it difficult for bacterial cells to destroy it. The biodegradation products are organic compounds of alcohol, aldehyde, carboxylic acids and a small proportion of aromatic hydrocarbon components. Keywords: performance; biodegradation; bacterial consortium; marine sponge; PAHs

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Systematic degradation mechanism and pathways analysis of the immobilized bacteria: Permeability and biodegradation, kinetic and molecular simulation

Cited by 39 publications

References 30 publications

Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

Comparison of the Strength of Biodegradation of Pyrene between Symbiont Bacteria of Marine Sponges and Bacteria Isolated from Seawater

Comparison of Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium Against Anthracene and Pyrene

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