Systematic characterization and proposed pathway of tetracycline degradation in solid waste treatment by Hermetia illucens with intestinal microbiota

Cai, Minmin; Ma, Shiteng; Hu, Ruiqi; Tomberlin, Jeffery K.; Yu, Chan; Huang, Yongping; Zhan, Shuai; Li, Wu; Zheng, Longyu; Zhou, Yu; Zhang, Jibin

doi:10.1016/j.envpol.2018.06.105

Cited by 91 publications

(55 citation statements)

References 41 publications

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“…In Indonesia, the black soldier fly was used for biowaste treatment which resulted in low direct GHG emissions compared to composting, while global warming potential was reduced (Mertenat et al, 2019). The black soldier fly is also able to degrade residual antibiotics, such as tetracycline in manure, which poses risks to ecosystems and public health (Cai et al, 2018) and pesticides (Lalander et al, 2016).…”

Section: Environmentmentioning

confidence: 99%

Insects as food and feed, a new emerging agricultural sector: a review

Huis

2020

JIFF

295

148

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During the last five years the scientific knowledge on insects as food and feed has been growing exponentially. At the same time, the industrial sector is increasingly engaged in rearing, processing and marketing of edible insects. Considerable attention is given to the black soldier fly as it can convert organic waste streams and transform it into several feed, food and industrial products. The farming of insects has an environmental impact which is lower than that of livestock species. The profitability of industrial production of insects as feed depends very much on the availability and applicability of cheap non-utilised side-streams. Microbial communities and their relationship with insects deserve full attention as it may help in the conversion of organic side streams of low economic value. Nutrition and health benefits for animals and humans need further exploration, also considering that insects have the largest anti-microbial peptide reservoir of all animals. Plant health can also be promoted by using chitin-containing leftover substrates as fertiliser. As insects have only recently been considered as food or feed, legislation trails developments. Therefore, politicians need to be assured that rearing and processing techniques are such that insect products are guaranteed free of chemical and microbial contaminants. Consumers are becoming more and more aware that insects as food are a viable option. Insects need to be processed into ingredients, that can be applied for safe and appetising products. The insect sector is maturing fast, but still faces many challenges, which can only be met when all stakeholders closely cooperate.

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Section: Environmentmentioning

confidence: 99%

Insects as food and feed, a new emerging agricultural sector: a review

Huis

2020

JIFF

295

148

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“…Abiotic conditions included food, heavy metals and antibiotics. These conditions will affect the conversion of BSF on waste, so many studies have focused on these conditions [8, 9, 28, 29]. As a result, a series of reference genes are recommended for gene expression studies in H .…”

Section: Introductionmentioning

confidence: 99%

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens)

2019

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Hermetia illucens is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of H . illucens reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including ATP6V1A , RPL8 , EF1 , Tubulin , TBP , GAPDH , Actin and RP49 ) was investigated under both biotic (developmental stages, tissues and sex) and abiotic (heavy metals, food, antibiotics) conditions. Gene expression data were analysed by geNorm, NormFinder, BestKeeper, and ΔCt programs. A set of specific reference genes was recommended for each experimental condition using the results of RefFinder synthesis analysis. This study offers a solid foundation for further studies of the molecular biology of H . illucens .

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“…Tetracycline (TC) was also a representative antibiotic [44]. BSF could degrade 97% tetracycline within 12 days [45]. However, effects of sulfonamide on BSF and sulfonamide degradation by BSF remained unclear.…”

Section: Introductionmentioning

confidence: 99%

Effect of sulfonamide pollution on the growth of manure management candidate Hermetia illucens

et al. 2019

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Antibiotics are commonly used in livestock and poultry farming. Residual antibiotics in manure may lead to antibiotic pollution of soil, surface water, and groundwater through land application and run-off rainfall. The black soldier fly (BSF) Hermetia illucens is a good candidate for manure management. We evaluated the effect of sulfonamide pollution on the growth of H . illucens . Four treatments were considered with a sulfonamide content in the feed of 0 (control group), 0.1, 1, and 10 mg/kg. The control larvae were fed without sulfonamide. Survival and development status of the individuals were recorded daily. The weights of the fifth instar larvae, prepupae, and pupae were checked. Antioxidant enzyme activity was determined with the fifth instar larvae. The results showed that a low (0.1 and 1 mg/kg) concentration of sulfonamides had no effects on larval survival, pupation, and eclosion of BSFs. A high sulfonamide concentration of 10 mg/kg had a significant effect on the survival of larvae and pupae and on the body weight of larvae, prepupae and pupae. Peak of the cumulated pupation rate and eclosion rate in the sulfonamide treatment of 10 mg/kg was very low. Pupation and eclosion in this group peaked later than that of the control and low sulfonamide concentration treatment groups (0.1 mg/kg and 1 mg/kg). Larvae from the sulfonamides group showed lower antioxidase activities than that of the control. In sulfonamide groups, the activity of peroxidase and superoxide dismutase was reduced in a concentration-dependent manner. Sulfamonomethoxine, sulfamethoxazole, and sulfamethazine were not detected in the harvested prepupae. Only sulfadiazine was discovered in the sulfonamide treatments of 1 and 10 mg/kg. In conclusion, BSFs can tolerate certain concentrations of sulfonamide contamination.

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Systematic characterization and proposed pathway of tetracycline degradation in solid waste treatment by Hermetia illucens with intestinal microbiota

Cited by 91 publications

References 41 publications

Insects as food and feed, a new emerging agricultural sector: a review

Insects as food and feed, a new emerging agricultural sector: a review

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens)

Effect of sulfonamide pollution on the growth of manure management candidate Hermetia illucens

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