Pomegranate (Punica granatum L.) is a perennial fruit crop grown since ancient times that has been planted worldwide and is known for its functional metabolites, particularly punicalagins. We have sequenced and assembled the pomegranate genome with 328 Mb anchored into nine pseudo-chromosomes and annotated 29 229 gene models. A Myrtales lineage-specific whole-genome duplication event was detected that occurred in the common ancestor before the divergence of pomegranate and Eucalyptus. Repetitive sequences accounted for 46.1% of the assembled genome. We found that the integument development gene INNER NO OUTER (INO) was under positive selection and potentially contributed to the development of the fleshy outer layer of the seed coat, an edible part of pomegranate fruit. The genes encoding the enzymes for synthesis and degradation of lignin, hemicelluloses and cellulose were also differentially expressed between soft- and hard-seeded varieties, reflecting differences in their accumulation in cultivars differing in seed hardness. Candidate genes for punicalagin biosynthesis were identified and their expression patterns indicated that gallic acid synthesis in tissues could follow different biochemical pathways. The genome sequence of pomegranate provides a valuable resource for the dissection of many biological and biochemical traits and also provides important insights for the acceleration of breeding. Elucidation of the biochemical pathway(s) involved in punicalagin biosynthesis could assist breeding efforts to increase production of this bioactive compound.
Several steady state measurement techniques are used to measure the film cooling effectiveness on a flat plate. Pressure sensitive paint (PSP), temperature sensitive paint (TSP), and infrared (IR) thermography are used to measure the film cooling effectiveness. To compare these measurement techniques, a single row of cylindrical holes, with a compound angle, are used. Seven holes (D = 4 mm) are equally spaced 12 mm apart, and the hole length-to-diameter ratio is 9.92. The axial angle (θ) of the holes is 30°, and the compound angle (β) is 45°. In addition to evaluating the various measurement techniques the effect of the coolant blowing ratio is considered; effectiveness measurements are taken for blowing ratios, M, of 0.4, 0.6, 1.2, and 1.8. The effect of mainstream turbulence intensity is considered with the addition of a turbulence grid to the low speed wind tunnel. Of the three steady state measurement techniques considered in this study, PSP demonstrates the most promise for the measurement of the film cooling effectiveness. Because PSP is a mass transfer technique, film effectiveness measurements can be readily obtained near the film cooling holes. Although the heat transfer techniques of TSP and IR thermography are more desirable than traditional thermocouples or liquid crystal thermography, the applicability of measurements near the holes is questionable due to conduction problems associated with steady state heat transfer techniques.
The expanded outer seed coat and the rigid inner seed coat of pomegranate seeds, both affect the sensory qualities of the fruit and its acceptability to consumers. Pomegranate seeds are also an appealing model for the study of seed coat differentiation and development. We conducted nontarget metabolic profiling to detect metabolites that contribute to the morphological differentiation of the seed coats along with transcriptomic profiling to unravel the genetic mechanisms underlying this process. Comparisons of metabolites in the lignin biosynthetic pathway accumulating in seed coat layers at different developmental stages revealed that monolignols, including coniferyl alcohol and sinapyl alcohol, greatly accumulated in inner seed coats and monolignol glucosides greatly accumulated in outer seed coats. Strong expression of genes involved in monolignol biosynthesis and transport might explain the spatial patterns of biosynthesis and accumulation of these metabolites. Hemicellulose constituents and flavonoids in particular accumulated in the inner seed coat, and candidate genes that might be involved in their accumulation were also identified. Genes encoding transcription factors regulating monolignol, cellulose, and hemicellulose metabolism were chosen by coexpression analysis. These results provide insights into metabolic factors influencing seed coat differentiation and a reference for studying seed coat developmental biology and pomegranate genetic improvement.
This paper is focused on the effect of film-hole configurations on platform film cooling. The platform is cooled by purge flow from a simulated stator-rotor seal combined with discrete-hole film cooling within the blade passage. The cylindrical holes and laidback fan-shaped holes are assessed in terms of film-cooling effectiveness and total pressure loss. Lined up with the freestream streamwise direction, the film holes are arranged on the platform with two different layouts. In one layout, the film-cooling holes are divided into two rows and more concentrated on the pressure side of the passage. In the other layout, the film-cooling holes are divided into four rows and loosely distributed on the platform. Four film-cooling hole configurations are investigated totally. Testing was done in a five-blade cascade with medium high Mach number condition (0.27 and 0.44 at the inlet and the exit, respectively). The detailed film-cooling effectiveness distributions on the platform were obtained using pressure sensitive paint technique. Results show that the combined cooling scheme (slot purge flow cooling combined with discrete-hole film cooling) is able to provide full film coverage on the platform. The shaped holes present higher film-cooling effectiveness and wider film coverage than the cylindrical holes, particularly at higher blowing ratios. The hole layout affects the local film-cooling effectiveness. The shaped holes also show the advantage over the cylindrical holes with lower total pressure loss.
Hermetia illucens
is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of
H
.
illucens
reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including
ATP6V1A
,
RPL8
,
EF1
,
Tubulin
,
TBP
,
GAPDH
,
Actin
and
RP49
) was investigated under both biotic (developmental stages, tissues and sex) and abiotic (heavy metals, food, antibiotics) conditions. Gene expression data were analysed by geNorm, NormFinder, BestKeeper, and ΔCt programs. A set of specific reference genes was recommended for each experimental condition using the results of RefFinder synthesis analysis. This study offers a solid foundation for further studies of the molecular biology of
H
.
illucens
.
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