Synthetic poly(amino acid) hydrogels with incorporated cell-adhesion peptides for tissue engineering

Abstract

Preparation of soft poly(amino acid) hydrogels containing biomimetic cell-adhesive peptides was investigated. Covalently crosslinked gels were formed by radical co-polymerization of methacryloylated macromonomer poly[N(5)-(2-hydroxyethyl)-L-glutamine-stat-L-alanine-stat-methacryloyllysine] with 2-hydroxyethyl methacrylate (HEMA) as minor co-monomer. Hydrogels carrying biomimetic peptides were prepared by using methacryloylated peptides, such as methacryloyl-GGGRGDSG-OH and methacryloyl-GGGYIGSR-OH, as additional monomers in the polymerization mixture. Mechanical stability and swelling in water of the hydrogels obtained for different solid:water and polypeptide:HEMA ratios were evaluated. The microporosity of gels (5-20 microm), dependent on the polyHEMA phase separation in water, was followed by low-vacuum SEM. The effect of biomimetic modification of hydrogels with RGDS and YIGSR peptides on the seeding efficiency of porcine mesenchymal stem cells (MSCs) was studied in vitro. While unmodified hydrogels showed very low cell adhesion, due to their highly hydrophilic nature, the incorporation of adhesive peptides significantly improved the adhesion and viability of seeded cells.

Biomimetic hydrogels as scaffolds for tissue-engineering applications

“…9.2c ) with ECMmimetic enzyme-sensitive degradation ( Table 9.2 ). For example, GPQGIAGQ (GIA), GPQGIWGQ, APGL and LGPA have been used for preparing matrix metalloproteinase (MMP)-sensitive hydrogels (Miller et al , 2010, Fosang et al , 1996, Bott et al , 2010, Lutolf et al , 2003c, YKNRD and VRN have been used for preparing plasmin-sensitive hydrogels (Jo et al , 2010, Halstenberg et al , 2002, and AAPVRGGG and AAAAAAA have been used for preparing elastase-sensitive hydrogels (Patel et al , 2005, West and Hubbell, 1999, Mann et al , 2001a, Patrick and Ulijin, 2010 Zhu et al, 2006, Zhu et al, 2009, Zhu and Marchant, 2008, Liu et al, 2009, Polizzotti et al, 2008, Liu et al, 2010, Zhou et al, 2009, Comisar et al, 2006, Studenovska et al, 2010, Herten et al, 2009 KQAGDV Fibronectin Integrin Peyton et al, 2006, Mann et al, 2001a, Mann and West, 2002 REDV Integrin α 4 β 1 Park et al, 2010, Massia and Hubbell, 1992, Girotti et al, 2004EILDV Masters et al, 2004 PHSRN Integrin α 5 β 1 Schmidt andKao, 2007, Benoit and LIGRKK Heparin Drake et al, 1993, Hansen et al, 1995SPPRRARV Woods et al, 1993WQPPRARI Woods et al, 1993…”

Designing degradable hydrogels for orthogonal control of cell microenvironments

“…314–317 The RGD ( Arg-Gly-Asp) sequence is found in a number of extracellular proteins, including fibrinogen, fibronectin, vitronectin, and laminin, and binds several integrins with varying strength. 318, 319 For spatiotemporally controlled presentation of the RGD motif in hydrogels, DeForest and Anseth employed SPAAC and thiol–ene click chemistries (Fig.…”

Entrapment and in vitro release of delta-sleep inducing peptide from polymer hydrogels based on modified polyvinyl alcohol

“…These include collagen [4], silk [5], polyhydroxybutirate [6], and synthetic polymers, such as polyesters of lactic and glycolic acids [7][8][9][10], polyamino acids [11], PVA [12,13], or their compos ites, for example, PVA alginate [14], PVA chitosan [15,16]. Polymer matrices could be prepared as * To whom correspondence should be addressed.…”