Designing degradable hydrogels for orthogonal control of cell microenvironments

Kharkar, Prathamesh M.; Kiick, Kristi L.; Kloxin, April M.

doi:10.1039/c3cs60040h

Cited by 594 publications

(538 citation statements)

References 377 publications

(585 reference statements)

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“…Ideally, the rate of degradation of the hydrogel would be optimized to match the degree of new tissue formation without any significant loss in mechanical strength. Incorporation of degradable moieties such as degradable polymer backbones, side groups or cross-link chains can be used to tailor degradation rate of the hydrogel [103]. The release of MMPs is common particularly among fibroblastic cells and is vital for cell translocation [104].…”

Section: Cell-mediated Remodelling Of Hydrogelsmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

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The development of hydrogel-based biomaterials represents a promising approach to generating new strategies for tissue engineering and regenerative medicine. In order to develop more sophisticated cell-seeded hydrogel constructs, it is important to understand how cells mechanically interact with hydrogels. In this paper, we review the mechanisms by which cells remodel hydrogels, the influence that the hydrogel mechanical and structural properties have on cell behaviour and the role of mechanical stimulation in cell-seeded hydrogels. Cell-mediated remodelling of hydrogels is directed by several cellular processes, including adhesion, migration, contraction, degradation and extracellular matrix deposition. Variations in hydrogel stiffness, density, composition, orientation and viscoelastic characteristics all affect cell activity and phenotype. The application of mechanical force on cells encapsulated in hydrogels can also instigate changes in cell behaviour. By improving our understanding of cell-material mechano-interactions in hydrogels, this should enable a new generation of regenerative medical therapies to be developed.

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Section: Cell-mediated Remodelling Of Hydrogelsmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

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“…Furthermore, substrate degradability may be desirable for both probing cell behavior and for in vivo use of engineered substrates. 24,112,113 A significant challenge remains engineering reversibility into these kinds of systems as opposed to one-directional changes. 114 Other factors such as dimensionality, 23,115 mechanical load, and shear flow are also potent regulators of cell behavior.…”

Section: Dynamic and Degradable Environmentsmentioning

confidence: 99%

“…ECM proteins and synthetic peptides enable more precise study of specific cell-ECM interactions. 5 Degradable 24 and dynamically tunable 25 platforms elucidate how cells react to changes in their microenvironments. Techniques such as 3D printing 26 and nanopatterning 27 allow for investigating processes on tissue and subcellular scales, respectively.…”

Section: Introductionmentioning

confidence: 99%

Capturing extracellular matrix properties in vitro: Microengineering materials to decipher cell and tissue level processes

Abdeen

Lee

Kilian

2016

Exp Biol Med (Maywood)

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Rapid advances in biology have led to the establishment of new fields with tremendous translational potential including regenerative medicine and immunoengineering. One commonality to these fields is the need to extract cells for manipulation in vitro; however, results obtained in laboratory cell culture will often differ widely from observations made in vivo. To more closely emulate native cell biology in the laboratory, designer engineered environments have proved a successful methodology to decipher the properties of the extracellular matrix that govern cellular decision making. Here, we present an overview of matrix properties that affect cell behavior, strategies for recapitulating important parameters in vitro, and examples of how these properties can affect cell and tissue level processes, with emphasis on leveraging these tools for immunoengineering.

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“…[1][2][3] Colloidal gels derived from reversibly associating microparticles are of potential value in these applications, for example as building blocks for scaffolds which can support tissue growth or as microcarriers for cell delivery. [4][5][6][7] In addition, reversibly-assembling colloidal gels might act as substrates on which to grow specific cells and then recover the resulting expanded population of cells following culture without the need to use trypsin during passaging, as the use of trypsin may affect cell phenotype preservation and gene expression.…”

Section: Introductionmentioning

confidence: 99%