Biomimetic hydrogels as scaffolds for tissue-engineering applications

Zhu, Junmin; Hu, Junli; Marchant, Roger

doi:10.1533/9780857098887.2.238

Cited by 8 publications

(13 citation statements)

References 201 publications

(144 reference statements)

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“…They are responsible for cell attachment, migration, adhesion, and differentiation [[65], [66], [67]]. A number of ligands, including LGTIPG, PDGSR, LRE, LRCDN, IKLLI, KQAGDV, and REDV, were extracted from ECM glycoproteins to promote cell adhesion [62,64,68,69].…”

Section: Functionalities Of Self-assembling Peptides Hydrogelsmentioning

confidence: 99%

Self-assemble peptide biomaterials and their biomedical applications

Chen

Zou

2019

Bioactive Materials

186

145

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Inspired by self-assembling peptides found in native proteins, deliberately designed engineered peptides have shown outstanding biocompatibility, biodegradability, and extracellular matrix-mimicking microenvironments. Assembly of the peptides can be triggered by external stimuli, such as electrolytes, temperature, and pH. The formation of nanostructures and subsequent nanocomposite materials often occur under physiological conditions. The respective properties of side chains in each amino acids provide numerous sites for chemical modification and conjugation choices of the peptides, enabling various resulting supramolecular nanostructures and hydrogels with adjustable mechanical and physicochemical properties. Moreover, additional functionalities can be easily induced into the hydrogels, including shear-thinning, bioactivity, self-healing, and shape memory. It further broaden the scope of application of self-assemble peptide materials. This review outlines designs of self-assembly peptide (β-sheet, α-helix, collagen-like peptides, elastin-like polypeptides, and peptide amphiphiles) with potential additional functionalities and their biomedical applications in bioprinting, tissue engineering, and drug delivery.

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Section: Functionalities Of Self-assembling Peptides Hydrogelsmentioning

confidence: 99%

Self-assemble peptide biomaterials and their biomedical applications

Chen

Zou

2019

Bioactive Materials

186

145

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“…Yen et al have also reported that keratinocytes were non-aggregative in their study, which inhibited the formation of epithelial aggregates, thereby decreasing the cellular interactions with the DP cells [ 16 ]. Short durations of culture are unable to sufficiently allow differentiation and re-programming of the keratinocytes into hair follicular fate by the DP.Gelatin, the degradation product of collagen, is one of the many natural biopolymers which mimic natural extracellular microenvironments and has been shown to enhance cellular responses and tissue regeneration [ 17 , 18 ]. Gelatin contains many arginine-glycine-aspartic acid (RGD) sequences that are important for cell attachment and act as target sequences of matrix metalloproteinase (MMP) useful for cell re-modelling [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating PEGDA and GelMA Microgel Models for Sustained 3D Heterotypic Dermal Papilla and Keratinocyte Co-Cultures

Tan

Nguyen²,

Common³

et al. 2021

IJMS

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Hair follicle morphogenesis is heavily dependent on reciprocal, sequential, and epithelial-mesenchymal interaction (EMI) between epidermal stem cells and the specialized cells of the underlying mesenchyme, which aggregate to form the dermal condensate (DC) and will later become the dermal papilla (DP). Similar models were developed with a co-culture of keratinocytes and DP cells. Previous studies have demonstrated that co-culture with keratinocytes maintains the in vivo characteristics of the DP. However, it is often challenging to develop three-dimensional (3D) DP and keratinocyte co-culture models for long term in vitro studies, due to the poor intercellular adherence between keratinocytes. Keratinocytes exhibit exfoliative behavior, and the integrity of the DP and keratinocyte co-cultured spheroids cannot be maintained over prolonged culture. Short durations of culture are unable to sufficiently allow the differentiation and re-programming of the keratinocytes into hair follicular fate by the DP. In this study, we explored a microgel array approach fabricated with two different hydrogel systems. Using poly (ethylene glycol) diacrylate (PEGDA) and gelatin methacrylate (GelMA), we compare their effects on maintaining the integrity of the cultures and their expression of important genes responsible for hair follicle morphogenesis, namely Wnt10A, Wnt10B, and Shh, over prolonged duration. We discovered that low attachment surfaces such as PEGDA result in the exfoliation of keratinocytes and were not suitable for long-term culture. GelMA, on the hand, was able to sustain the integrity of co-cultures and showed higher expression of the morphogens overtime.

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“…In vivo, the ECM provides mechanical support for cells . In addition to cell–cell interactions, the matrix–cell interactions play fundamental roles in regulating cellular functions, including adhesion, morphogenesis, migration, proliferation, differentiation, and gene expression. , …”

Section: Introductionmentioning

confidence: 99%

“…13 In addition to cell−cell interactions, the matrix−cell interactions play fundamental roles in regulating cellular functions, including adhesion, morphogenesis, migration, proliferation, differentiation, and gene expression. 14,15 The binding ligands within ECM provide anchorages for cell adhesion and connect the cytoskeletons of cells to the surrounding environment so that cells can sense the mechanical and biochemical changes and other signaling processes. In order to improve cell viability and function, it is necessary to integrate bioactive ligands to interact with cells when designing and constructing hydrogel networks.…”

Section: Introductionmentioning

confidence: 99%

Injectable Polypeptide Hydrogel as Biomimetic Scaffolds with Tunable Bioactivity and Controllable Cell Adhesion

Zhang

Xiao

et al. 2017

Biomacromolecules

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Injectable hydrogels have been widely investigated for applications in biomedical fields, for instance, as biomimetic scaffolds mimicking the extracellular matrix (ECM). In addition to as scaffolds for mechanical support and transferring of nutrients, the dynamic bioactivity of ECM is another critical factor that affects cell behavior. In this work, a novel injectable poly(l-glutamic acid)-based hydrogel decorated with RGD was fabricated. The presentation of RGD significantly enhanced the cell-matrix interaction and promoted cell adhesion and proliferation. Moreover, the cell-adhesive RGD was conjugated to the network via a disulfide bond, so that the density of RGD and the bioactivity of hydrogel can be well controlled by tuning the RGD content through treating with glutathione. As a result, the cell behaviors on the hydrogel can be tuned on demand. The injectable hydrogel with controllable bioactivity may provide an interesting strategy to develop a scaffold mimicking ECM that can regulate cell adhesion dynamically.

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Biomimetic hydrogels as scaffolds for tissue-engineering applications

Cited by 8 publications

References 201 publications

Self-assemble peptide biomaterials and their biomedical applications

Self-assemble peptide biomaterials and their biomedical applications

Investigating PEGDA and GelMA Microgel Models for Sustained 3D Heterotypic Dermal Papilla and Keratinocyte Co-Cultures

Injectable Polypeptide Hydrogel as Biomimetic Scaffolds with Tunable Bioactivity and Controllable Cell Adhesion

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